Hey friends,
I have a micro array experiment, I am going to pre-process it using the MAS 5.0 algorithm and align to build a matrix in the 'R' then Probe sets that were found 'absent' on ≥ 10 % of microarray chip to will be omitted from further analysis (i don't know how do this also). I installed R....
Right now after hours searching, I got fully puzzled, please don't leave me alone in this step
Thanks
2 answers
Well, if you are going to do this in R and you have not used R before, you'll likely need to do a little learning online. Then, you can pick up with Bioconductor and perhaps start with this page for affymetrix oligo arrays.
http://bioconductor.org/help/workflows/arrays/
If or when you get stuck, you can write back with more specific questions.
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written
by
Sean Davis
Here .. Try this tutorial. They explain how to use mas5 to remove absent probes.
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written
by
poisonAlien
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