Promoter Motifs (+) and (-) strands
Hello,
I have been researching on putative motif on a promoter region. PLACE (http://www.dna.affrc.go.jp/PLACE/index.html) signal scan gave me results like this.
(+) = Current Strand
(-) = Opposite Strand
1 TGTGCCCACCACAGCACACCTATCGTTATCATCAGCGTGGACTAAGAACA
(-)GATAMOTIF S000039
(-)GATAMOTIF S000039
51 GAACAATTTCTCATCTTTTGTTTCCTGAGAAGTTTAGACCACCAAGATAT
(+)CAATBOX1 S000028
(+)GATAMOTIF S000039
101 ATGCTGATGATGCTGTAGATAGCTAGCTAGTTTTGCAATTACTTATGGGT
(+)GATAMOTIF S000039
(+)CAATBOX1 S000028
(-)SEF3MOTIF S000115
151 TGCATCACAATCAGGGTCTGTTTAGTTCCCAAACAAAATTTTTCACGCTG
(+)CAATBOX1 S000028
(-)-300ELEMENT S000122
201 TTACATAGGATGTTTGGACACATGCATAGAGTACTAAATGTAGAAAAAAA
(+)EBOXBNNAPA S000144
(-)EBOXBNNAPA S000144
(+)RYREPEAT1 S000100
(+)RYREPEAT2 S000105
251 ACAATTAAACATTTCGCCTTGAAATTACGAGACAAATCTTTTAAGCCTAA
(+)CAATBOX1 S000028
(+)POLASIG2 S000081
My gene is in (+) strand and motifs will be mapped. So should I consider motifs (-) strand too? Do (-) strand motifs affect a gene in (+) strand?
1 answer
Yes you should consider motifs on the minus strand to be as important as those on the plus strand. There is a large body of research on bidirectional promoters.
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mark.ziemann
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