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Illumina® HT 12 v4 BeadChip arrays- reasonable replication, comparison of samples across slides
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5.8 years ago
yzatod • 0

Hello,

We would like to carry out an experiment on a cell line treated with cytoprotective agents. Given state of technology and analysis algorithms (normalization, differential gene expression analysis) what is a reasonable level of biological replication? I noticed that many paper cite 3 arrays per condition, which suprised me, I would expect higher numbers. But I would not object to lower numbers if it would be seen as quite standard :).

-- I am aware that biological variability is key factor.

Also Illumina offer 2 slides by 12 chips. Is it reasonable to make cross-slide comparisons?

thanks for guidance

yza

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