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Calculate saturation on nucleotide MSA

Hi would like to check for possible saturation of nucleotide substitution types on several nucleotide MSA.

I know that Xia et al published an index and this is implemented in DAMBE5

However, as far as I get I should submit my MSA one by one in DAMBE, and this would be infeasible for me. Do you know any other methods I could use and iterate in Linux?

phylogeny alignment

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