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SNP mining from ESTs

Can any one tell me procedure or a flow chart showing mining of SNPs from EST data set through SNPdigger, SEAN, autoSNP, or any software.

Thanking in advance

est-snp snp

1 answer

It's a good idea that mining of SNPs from EST data set.

As far as I can see, EST is just a sequencing read range from 400bp~1.6kb , why should we use the tools you list, just align it to refer genome, and find the diff, which will be the snp you want.

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