SNP mining from ESTs

Question

[dinesh]

Can any one tell me procedure or a flow chart showing mining of SNPs from EST data set through SNPdigger, SEAN, autoSNP, or any software.

Thanking in advance

Answer 1

[jimmy_zeng]

It's a good idea that mining of SNPs from EST data set.

As far as I can see, EST is just a sequencing read range from 400bp~1.6kb , why should we use the tools you list, just align it to refer genome, and find the diff, which will be the snp you want.