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Assembly-free metagenomics tools

We would like to set up a bioinformatic method to analyse high throughput metagenomics data avoiding the assembly step. Our objective is to get taxonomy and functional information of metagenomics samples.

I open this thread for people to post this kind of tools.

Thanks,

Although some papers have been published, seems this strategy is under development:

Luo, Haiwei, and Mary Ann Moran. "Assembly‐free metagenomic analysis reveals new metabolic capabilities in surface ocean bacterioplankton."Environmental microbiology reports 5.5 (2013): 686-696.

Sunagawa, Shinichi, et al. "Metagenomic species profiling using universal phylogenetic marker genes." Nature methods 10.12 (2013): 1196-1199.

metagenomics

3 answers

MG-RAST does everything for you. Keep in mind though that taxonomic and functional assignments from short reads come with low statistical support..

You should also look "Kraken"

AmporaNet and AmphoraVizu are simple and great online tools for analysis of assembly-free shotgun sequenced metagenomic data.

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