how to get TF binding affinity from ENCODE narrowPeak or broadPeak files?
I just learn to understanding ENCODE ChiP-Seq data for Transcription Factor binding. I know the narrowPeak format consists BED6+4 columns which is described in https://genome.ucsc.edu/FAQ/FAQformat.html#format12. My question is:
- Does the region between chromStart and chromEnd mean the peak region (binding region)?
- Does the signalValue mean the binding affinity?
- Is it good to compare this value across cell lines to see the difference?
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