I got it. Thx a lot:p
Hi,
I've completed alignment with Tophat2, and got some output files, including deletions.bed, junctions.bed and insertions.bed. My problem is how to tell what info it is in each file. I've checked the instruction of UCSC BED file, but I still cannot make it clear. My deletions.bed file content is like:
track name=deletions description="TopHat deletions"
AGTP01133720.1 1571 1573 - 1
AGTP01133720.1 3360 3362 - 1
AGTP01133720.1 14859 14860 - 1
AGTP01133720.1 15434 15435 - 1
AGTP01133720.1 19755 19756 - 1
AGTP01133720.1 20696 20697 - 2
AGTP01133720.1 22781 22782 - 1
AGTP01133720.1 23171 23172 - 1
So, what is it in the 1st column? Any explanation will be appreciated.
1 answer
Hi leo,
I don't know if I understand correctly your question but, in the first column you have the chromosome (scaffold, or contig), where the deletion takes place.
I guess I have a more basic question than this: What are the deletions? Like, what in the genome do they represent? Are they reads that mapped but the read had a deletion relative to the reference?
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