Hi all, I am using samtools collate to convert my bam files to paired end fastq files. here is the command that I am using …

hello everyone, I am analyzing some small RNA sequenced with an UMI and I use STAR to map them. I want to conserve the multimappers …

<p>Hi everyone, </p> <p>I don't know which column change when we fixmate the reads using PICARD? could you please tell me what is fixmate? (I …

<p>Hello, </p> <p>I want to align the small RNA seq from illumina. is BWA a good mapper for small RNA? </p> <p>Thanks Sara</p>

<p>Hi, </p> <p>could you please tell me where i can find the adaptor sequence of illumina small RNA (miRNA) kit? is it universal, I means …

<p>Hi everyone, </p> <p>I did a heatmap using library(gplots) in R. I have a very nice plot but I don’t know how many clusters are …

<p>I was reading that about MAQ and I found out that it does an alignment step. My understanding of MAQ is:</p> <p>reads --&gt; MAQ --&gt; …

<p>hey, </p> <p>is there any bed file format for Brachypodium_distachyon.Brachy1.0.cdna.all.fa?</p> <p>Thanks sara</p>

<p>Hi,</p> <p>I want to convert map-view file (output of Maq) to bed file.</p> <p>is there any option in Maq I should consider?</p> <p>Thanks in advance …

<p>Hi, </p> <p>I did the mapping of small miRNA seq with Maq. How I can annotate my miRNA? I want to know if the mapped …