rps blast database 'formatrpsdb' problem
Hi..
After installing BLAST+ package from ncbi, when I try to build a database for rps blast analysis using:
formatrpsdb -t COG_first -i Cog.pn -o T -f 11 -n COG -S 100.0
it gives following error:
[NULL_Caption] FATAL ERROR: COG0001.smpInput
PssmWithParameters.pssm.intermediateData.freqRatios
Unable to match element in intermediateData. line 24227
[NULL_Caption] ERROR: no data found in file 'COG0001.smp'
Although I check all files needed to run the program are available.
Please suggest a solution.
Thank you
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User 6777 •
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1 answer
Instead of formatrpsdb use makeprofiledb:
makeprofiledb -in Cog.pn -title Cog -out Cog -dbtype 'rps'
Where Cog.pn is a list of the smp files you want to include and -title and -out is how you want your new database to be named.
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allison.e.mann •
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