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Eukaryotic genome annotation steps

Hi,

I have some whole genome shot gun contigs. Now I want to annotate those contigs. So my question is what are exacty protocol to annotate a eukarotic genome. Should I clean the contigs by removing the non coding RNA sequences, transposable lemnets etc. and then go for gene prediction programs or I can directly go gene prediction programs

sequencing genome
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updated by Ram Ram • written by prp291 prp291

1 answer

"A beginner's guide to eukaryotic genome annotation"

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updated by Ram Ram • written by Zev.Kronenberg Zev.Kronenberg

I know this paper but they haven't mentioned aboout transposable element prediction or non coding RNA sequence removal. Is this not a necessary step?

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written by prp291 prp291

Look at the section in the paper: STEP 1

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updated by Ram Ram • written by Zev.Kronenberg Zev.Kronenberg

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