How to use maximum identity > 95%, length > 200 bp as search parameters for tblastn standalone version
Hello guys,
Could someone please suggest to me how to use following search parameters for tblastn search (stand alone version)
Search parameters : maximum identity > 95%, length > 200 bp and Evalue < 10e-10.(borrowed idea from http://www.biomedcentral.com/1471-2164/12/76; Digital gene expression section)
I am well conversant with blast commands such as:
tblastn -db blastdb/nippest -query mydata.fasta -evalue 10e-10 >out.nippest - outfmt 11
Following the attached paper I wish to add maximum identity >95% and length 200 bp as part of search parameters but I can't figure out how to go about. I have checked all NCBI BLAST documentations but I can't find the solution.
Thanks
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Seems duplicate of your own previous question
Yes, it is... I wrote the first question by mistake on the comment section of another question I had asked earlier on (gene duplication) by mistake and couldn't find a way to delete it....
That's why I posted it as new post. But I have figured out how to delete it.Thanks.