I am planning a 10X single cell RNAseq library preparation and sequencing in Nova seq. For human genome to look at standard differential expression and …

Understanding how NGS works usually requires translating technology jargon and stitching together tidbits from presentations or discussions with colleagues. This handbook is designed to assist …

**Guides for sequencing** [Designing a sequencing project][1] [Recommended coverage by library preparation application][2] [Comparison of instrument read lengths and read outputs][3] **Guides for preparing your …

Genohub is in the process of developing an evolving coverage and read depth guide: https://genohub.com/recommended-sequencing-coverage-by-application/ based on references in the field. We'd like to ask …

Unfortunately, we estimate ~80% of those doing DGE use only 3 replicates. At least 6 replicates should be used to account for variability, bad replicates …

~ 11% of NCBI's Gene Expression Omnibus (GEO) projects between 2012 and 2013 contain at least >= 100 reads / million reads mapping to mycoplasma's …

Day 1 of the Sequencing, Finishing and Analysis Meeting kicked off with a great series of lectures. We've highlighted some of the talks for those …

Demand for the analysis of next generation sequencing data is growing at a fast rate. To help accommodate this demand, [Genohub.com][1] now allows researchers to …

<p>Interesting paper examines transcriptome reassembly and determines it is sensitive to RNA fragmentation technique. Concludes that Zn mediated fragmentation provides a better transcript ID compared …

AGBT 2014 started off strong with talks on single cell sequencing, next gen diagnostics in cancer medicine and GRCh38. A twitter/blog embargo threw a wrench …