Error loading blast table (outfmt 6 option) in Megan 5

Question

[fhsantanna]

Hi,

I am trying to load the following blast table in Megan5:

01contig00001_1_491_+    gi|495059223|ref|WP_007784057.1|    29.94    157    100    4    9    157    4    158    2e-11    67.4    1144311
01contig00001_567_4372_+    gi|495059216|ref|WP_007784050.1|    56.09    624    262    7    348    967    5    620    0.0     711    1144311

When I import this table together with gi mapping file (http://ab.inf.uni-tuebingen.de/data/software/megan5/download/welcome.html), it only appears a "tree" with not assigned taxa.

As you can see in the picture that I uploaded (http://postimg.org/image/6ege4uzjx/), the sequences were identified, a taxon name was attributed to each one. However, I don't understand why in the main window they are named as not assigned...

Could somebody help me? I already read the manual, but it is not tottaly clear to me.

Thank you in advance.

[lelle]

Is this your actual data or just a minimal example? Did you set the min support parameter? With default paramters 2 sequences is probably not enough to satisfy the min support rule and the assignments get rejected.

[fhsantanna]

Yes. After I realized it. Thank you very much Leile for the feedback.

One more question, where do I find the Gi map file for the SEED analysis?

Thanks!