Error loading blast table (outfmt 6 option) in Megan 5
Hi,
I am trying to load the following blast table in Megan5:
01contig00001_1_491_+ gi|495059223|ref|WP_007784057.1| 29.94 157 100 4 9 157 4 158 2e-11 67.4 1144311
01contig00001_567_4372_+ gi|495059216|ref|WP_007784050.1| 56.09 624 262 7 348 967 5 620 0.0 711 1144311
When I import this table together with gi mapping file (http://ab.inf.uni-tuebingen.de/data/software/megan5/download/welcome.html), it only appears a "tree" with not assigned taxa.
As you can see in the picture that I uploaded (http://postimg.org/image/6ege4uzjx/), the sequences were identified, a taxon name was attributed to each one. However, I don't understand why in the main window they are named as not assigned...
Could somebody help me? I already read the manual, but it is not tottaly clear to me.
Thank you in advance.
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SEED assignment (MEGAN5)
written by fhsantanna
Is this your actual data or just a minimal example? Did you set the min support parameter? With default paramters 2 sequences is probably not enough to satisfy the min support rule and the assignments get rejected.
Yes. After I realized it. Thank you very much Leile for the feedback.
One more question, where do I find the Gi map file for the SEED analysis?
Thanks!