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assigning function to putative/hypothetical proteins

Hi All,

What is the best and current bioinformatics approach to assign functions to putative/hypothetical proteins?

Thanks

sequence alignment blast rna-seq
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updated by Prakki Rama Prakki Rama • written by robjohn7000 robjohn7000

1 answer

  1. You can always choose BLAST to assign function to your unknown sequence. (also can try programs reciprocal blast for better conviction, inparanoid, RSD if query is proteins)
  2. Sometimes, based on domains also, the functions can be assigned. (can try interproscan)

A tool called BLAST2GO has some easy to use features.

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updated by Ram Ram • written by Prakki Rama Prakki Rama

+1 for interproscan

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updated by Ram Ram • written by Asaf Asaf

Many thanks for your suggestions Prakki

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updated by Ram Ram • written by robjohn7000 robjohn7000

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