Vector screening of EST sequence for cotton
I am working on analysis of EST sequences for cotton, extracted form dbEST, NCBI. For removal of vector contamination i am using VecScreen. I have subjected approximate 10,000 EST sequences to VecScreen. But every time VecScreen produces same result i.e. "No significant similarity found".
Is It ok.
or i should try any other vector contamination removal tool.
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Ram
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meghasihag •
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meghasihag •
1 answer
try to grep a portion of your vector or do a simple BLAT and see, if it is capturing any thing similar to vector. If the hits are spurious you could be sure of no vector in your EST data.
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Prakki Rama
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Prakki Rama
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