Wanting to make a Venn diagram of ATACseq peak files, unsure of where to start
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4 weeks ago
Ronin • 0

I've got some ATACseq peak files that I've generated (both from Homer and MACS2, in case one is preferred). I am wanting to generate a Venn diagram to compare/contrast these peaks. How might I be able to do this? I was hoping to then put this input file into an interactive app like from here:

https://asntech.shinyapps.io/intervene/

The example dataset used on the above website is:

mESC    pro-B   Myotube Th-cell
NM_001081209    NM_008922   NM_133810   NM_001109661
NM_133833   NM_008922   NM_028133   NM_028331
NM_015818   NM_145996   NM_010866   NM_016754
NM_015818   NM_019790   NM_026830   NM_007598
NM_018872   NM_033563   NM_028040   NM_008856
NM_001005423    NM_027456   NM_001163360    NM_001081345
NM_008342   NM_027966   NM_178665   NM_010735
NM_025386   NM_008972   NM_022318   NM_001162366
NM_010262   NM_010566   NM_015740   NM_024214
NM_023755   NM_177646   NM_175418   NM_021782

For reference, my own peak files look something like this (from Homer):

#PeakID chr start   end strand  score   focus ratio/other   Total Tags  Background Tags Fold Change vs. Background  p-value
Merged-VHII01000031.1-133075-1  VHII01000031.1  133025  133125  +   463 0   1499.5  215.9   6.94    0.00E+00
Merged-CM020919.1-145512-1  CM020919.1  145462  145562  +   285.5   0   53  8.3 6.37    4.01E-25
Merged-CM020928.1-3483966-1 CM020928.1  3483916 3484016 +   188.4   0   422 22.5    18.74   0.00E+00
Merged-CM020931.1-7099184-1 CM020931.1  7099134 7099234 +   163.7   0   380 69  5.5 9.51E-149
Merged-VHII01000031.1-132864-2  VHII01000031.1  132797  132932  +   162.6   0   1711.5  248.2   6.89    0.00E+00
Merged-CM020911.1-23060979-1    CM020911.1  23060929    23061029    +   157.7   0   398.5   45.5    8.75    4.93E-224
Merged-CM020922.1-34104023-1    CM020922.1  34103973    34104073    +   150.7   0   40  2.4 16.34   4.03E-34
Merged-CM020920.1-21165501-1    CM020920.1  21165451    21165551    +   131.7   0   186 25  7.45    1.80E-94
Merged-CM020925.1-35916431-1    CM020925.1  35916381    35916481    +   129.3   0   267 64.6    4.13    7.97E-79
Merged-CM020921.1-28176661-2    CM020921.1  28176578    28176745    +   124.4   0   689.5   72  9.58    0.00E+00

This is just a merged file of one type of tissue (I have three total).

Thanks in advance for any insights you might be able to provide.

ATAC-seq • 140 views
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Something more global and useful based on positions: https://bedtools.readthedocs.io/en/latest/content/tools/jaccard.html

That might be useful.

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