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675 results • Page
2 of 14
Sort: Views
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Views
Votes
Replies
3
votes
6
replies
2.1k
views
7 follow
DNA methylation preprocessing
SWAN
DNA
methylation
Funnorm
updated 29 days ago by
Ram
44k • written 2.4 years ago by
SYOSY
▴ 10
2
votes
6
replies
2.0k
views
How can you *consistently* download BioProject IDs from NCBI's BioSample database using Entrez Direct?
XML
ncbi
entrez-direct
updated 10 days ago by
Ram
44k • written 3.9 years ago by
millere
• 0
6
votes
6
replies
1.9k
views
agilent human all exon sureselct v7 bed file
exon
human
bed
v7
agilent
all
sureselect
updated 20 days ago by
MiladAD
▴ 10 • written 2.0 years ago by
soheil
• 0
2
votes
2
replies
1.8k
views
Parametric or non-parametric tests for qPCR significance testing between treatments?
qPCR
statistical test
parametric
non-parametric
updated 22 days ago by
e.r.zakiev
▴ 210 • written 3.9 years ago by
n.anuragsharma
▴ 40
0
votes
3
replies
1.8k
views
Running STRUCTURE from command line
STRUCTURE
updated 4 days ago by
Ram
44k • written 3.0 years ago by
giulia.trauzzi
▴ 10
6
votes
5
replies
1.8k
views
How to calculate coverage of Nanopore long read data?
sequencing
coverage
11 days ago by
Arton
▴ 10
1
vote
2
replies
1.7k
views
Seurat CellCycleScoring – confused about the proper order of operations when using SCTransform
CellCycleScoring
SCTransform
Seurat
updated 17 days ago by
Li
• 0 • written 2.3 years ago by
GPM
▴ 10
4
votes
15
replies
1.7k
views
how to test for differential expression in samples where a global increase in gene expression is expected
edgeR
DESeq
Differential-Expression
RNA-Seq
updated 22 days ago by
Gordon Smyth
★ 7.2k • written 5 months ago by
raplayer
▴ 10
0
votes
9
replies
1.5k
views
kinannote software installation problems
kinannote
updated 17 days ago by
Emanoelle
• 0 • written 5.8 years ago by
Elizabeth
▴ 30
1
vote
6
replies
1.5k
views
CellRanger output more cells than specified using --force-cells? Why?
scRNA-seq
cellranger
updated 7 days ago by
scideas
▴ 30 • written 8 months ago by
rohitsatyam102
▴ 870
2
votes
7
replies
1.4k
views
6 follow
Differential Expression using Isoseq-supplemented reference transcriptome
RNA-Seq
Salmon
Isoseq
updated 27 days ago by
Gordon Smyth
★ 7.2k • written 9 months ago by
Calum
▴ 10
1
vote
2
replies
1.3k
views
Extracting named fasta sequences according to list with Biopython
Biopython
FASTA
updated 12 days ago by
Rubayetul
• 0 • written 2.6 years ago by
lachiemck
• 0
8
votes
16
replies
1.3k
views
How to convert plink files to Hapmap Format
GWAS
Plink
updated 29 days ago by
bk11
★ 2.5k • written 11 weeks ago by
Sofia
• 0
4
votes
6
replies
1.2k
views
Soft-clipping read ends based on read group
ancient
molecule
at
bam
DNA
RG
ends
clipping
damage
updated 17 days ago by
chenl
▴ 10 • written 2.6 years ago by
Martyna
• 0
1
vote
2
replies
1.2k
views
News:
The GDC Legacy Archive is retiring soon.
genomic-data-commons
tcga
gdc
21 days ago by
Zhenyu Zhang
★ 1.2k
2
votes
4
replies
1.2k
views
Is it possible to run CibersortX with a very small number of samples?
cibersortx
2 days ago by
Aspire
▴ 330
1
vote
4
replies
1.2k
views
Filtering qscore on dorado
dorado
filtering
QC
nanopore
Guppy
23 days ago by
eebloom
▴ 80
3
votes
0
replies
1.1k
views
Tool:
Sars-Cov-2 Genome & ACE 2 Exploration
sequence
alignment
SNP
genome
20 days ago by
Ibrahim Tanyalcin
★ 1.2k
2
votes
10
replies
1.1k
views
HGVS Nomenclature of Multiple Indels found in Cis
Nomenclature
HGVS
updated 5 days ago by
Ram
44k • written 5 months ago by
LauferVA
4.2k
13
votes
14
replies
1.1k
views
High Malat-1 expression in single cell data
single-cell
updated 18 days ago by
t.montserrat.ayuso
▴ 40 • written 26 days ago by
carolofharvest
▴ 40
2
votes
11
replies
1.1k
views
Questions about a bug when transferring cram file to bam file
sequence
samtools
bcftools
updated 27 days ago by
jkbonfield
★ 1.2k • written 5 weeks ago by
me
• 0
7
votes
16
replies
1.0k
views
How many reads for WGS Sequencing?
WGS
Bacterial-Genomics
27 days ago by
Ruqaiya
• 0
2
votes
5
replies
967
views
Retrieval of Active site information programmatically
Catalytic
Python
Active
PDB
site
Site
updated 26 days ago by
Wayne
★ 2.0k • written 2.1 years ago by
arinjoy
• 0
4
votes
11
replies
951
views
Create a new bed file with all pairwise combinations between two other bed files, based on bp distance
SNPs
BED
eqtl
bedtools
4 days ago by
J
▴ 10
1
vote
10
replies
922
views
error to run rnammer
phylogenomics
rRNA_extraction
rnammer
updated 20 days ago by
antonio.spl
• 0 • written 4 months ago by
microorganism_001
▴ 30
1
vote
3
replies
910
views
Which 1000 genomes 30x files should I use for imputation?
1000genomes
imputation
SNP
updated 3 days ago by
David-walson
• 0 • written 16 months ago by
Apprentice
▴ 160
2
votes
3
replies
894
views
Issues with Mixture file when using CIBERSORTx
Deconvolution
CIBERSORTx
updated 21 days ago by
vjanve
• 0 • written 7 months ago by
mateomejias
• 0
3
votes
8
replies
876
views
Random Access remote BAM files
htslib
BAM
updated 3 days ago by
a.penatauber
• 0 • written 3 months ago by
Lucas R.F.
▴ 20
10
votes
14
replies
860
views
6 follow
Bacterial plasmid analysis
bacteria
plasmid
wgs
hybridassembly
sequencing
updated 18 days ago by
GenoMax
142k • written 24 days ago by
nicole.kavanagh
• 0
4
votes
6
replies
840
views
7 follow
Heatmap and rna-seq
RNA-Seq
Heatmap
updated 29 days ago by
dsull
★ 6.1k • written 4 weeks ago by
qudrat.nii
▴ 10
1
vote
5
replies
827
views
Converting IGV to Figure using Illustrator (or other tools)
Illustrator
IGV
written 6 months ago by
Daniel
▴ 30
1
vote
16
replies
826
views
Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NULL > pointer.
blast
ncbi
updated 7 days ago by
GenoMax
142k • written 10 days ago by
hophuquy0944
• 0
7
votes
13
replies
826
views
7 follow
Forum:
Ideal PC configurations and operating system for bioinformatics laboratory
PC
updated 2 days ago by
i.sudbery
19k • written 4 weeks ago by
Estevão
▴ 10
1
vote
3
replies
820
views
About runing Pilon on individual chromosomes when polish draft genome assembly
Pilon
chromosome
polish
genome
draft
updated 9 days ago by
sansan_96
▴ 90 • written 2.8 years ago by
boymin2020
▴ 80
0
votes
1
reply
813
views
cnetplot category names are too long
clusterprofiler
RNA-Seq
enrichplot
cnetplot
updated 23 days ago by
Ram
44k • written 2.7 years ago by
carov
• 0
2
votes
11
replies
806
views
DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts in whole blood: is it normal to always get NS?
rstudio
DEG
limma
9 days ago by
egascon
• 0
0
votes
1
reply
795
views
Can I run cellassign on samples independently if there is batch effect present?
RNA-Seq
R
cellassign
batch-effect
updated 27 days ago by
Francesco
▴ 10 • written 3.4 years ago by
gt
▴ 30
0
votes
3
replies
794
views
Why cd-hit-est not work when sequence identity threshold<0.95?
genome
cluster
sequence
updated 26 days ago by
weidonglu
• 0 • written 22 months ago by
JZX
• 0
2
votes
9
replies
794
views
Filtering Multi-sample VCF file for all except one Genotype
variant
SNP
VCF
updated 11 days ago by
Pierre Lindenbaum
162k • written 18 days ago by
schmince
• 0
1
vote
6
replies
782
views
anRichment is missing
WGCNA
anRichment
updated 19 days ago by
GenoMax
142k • written 6 months ago by
michael.flower.14
▴ 180
0
votes
2
replies
764
views
homer not configured properly
Homer
makeTagDirectory
updated 23 days ago by
clairechung112
• 0 • written 2.0 years ago by
amahdi779
• 0
0
votes
1
reply
758
views
Job:
Seeking Bioinformatics/Drug Design Opportunities - PhD in Pharmacology with Experience in RNA-seq, CADD & Deep Learning Molecular Generation
search
job
updated 20 days ago by
GenoMax
142k • written 21 days ago by
tulip
• 0
3
votes
8
replies
757
views
Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA2 - where to go from here?
nf-core
16S
amplicon
dada2
ampliseq
updated 19 days ago by
Chris Dean
▴ 410 • written 23 days ago by
sovrappensiero
▴ 100
1
vote
1
reply
741
views
Herald:
The Biostar Herald for Monday, November 01, 2021
herald
written 2.6 years ago by
Biostar
2.8k
1
vote
2
replies
734
views
Checking chromosome builds for genotyping data
GWAS
Liftover
PLINK
updated 7 days ago by
Muhammad
• 0 • written 2.6 years ago by
mari.johnson
• 0
2
votes
4
replies
733
views
Count all variants from vcf file
variants
vcf
Count
updated 19 days ago by
Pierre Lindenbaum
162k • written 21 months ago by
t.ali
• 0
0
votes
1
reply
732
views
after selecting specific GO terms to plot, cneplot is not showing any color for genes, GO terms and only showing lines without nodes.
cneplot
updated 25 days ago by
Ram
44k • written 25 days ago by
Dr Huma Naz
• 0
0
votes
3
replies
713
views
Pluritest for pluripotency broken
pluripotent
pluritest
cells
stem
updated 17 days ago by
DGTool
▴ 20 • written 22 months ago by
yassine
• 0
5
votes
5
replies
704
views
Tutorial:
how to combine multiple RNAseq count files into a single dataframe in R and unix
Unix
RNAseq
R
updated 22 days ago by
Mbofire
• 0 • written 4 weeks ago by
Ming Tommy Tang
★ 3.9k
0
votes
9
replies
701
views
Applying the metacell2 algorithm using python
python
single-cell
scanpy
metacell2
updated 10 days ago by
Wayne
★ 2.0k • written 19 days ago by
JACKY
▴ 140
675 results • Page
2 of 14
Recent Votes
Low DESeq2 Sensivity on scRNA Pseudobulk Samples
DESEQ2: How can I convert ensembl gene id's to HGNC gene names.
Problem with Ensembl version identifiers after running DESeq2
Issues with adding gene name and entrez ID to DESeq2 result having Ensembl ID.
A: Looking for differential gene expression between treatment, within a specific cl
How to compare cell-type ratios in sc-RNA seq data?
How to calculate cell type frequency between two groups in single cell data
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Recent Replies
Comment: Merging multiple samples in Seurat
by
Picasa
▴ 640
did you split by sample or donor ?
Comment: Harmony integration group.by.var parameter
by
Picasa
▴ 640
For downstream analysis, after integration, my plan is to annotate each cluster/cell type and then perform a DGE analysis of KO versus WT f…
Comment: How to calculate cell type frequency between two groups in single cell data
by
Bastien Hervé
5.3k
That is correct
Answer: Setting a threshold on gene expression for subsetting (scRNA-seq)
by
ATpoint
82k
That depends on the data and the marker. Generally I would plot violins per cluster for the marker(s) and see whether one robustly overexpr…
Comment: Error when i use EVidenceModeler to do the genome annotation
by
peanut
• 0
thank you so much ,i get it
Comment: Number of non-ATCG nucleotides replaced by Salmon
by
Tonya S.
▴ 10
Oops, yes, that must be where they are coming from. For some reason, I was thinking the genome was just soft-masked. How embarrassing! Than…
Answer: Number of non-ATCG nucleotides replaced by Salmon
by
Rob
6.6k
If there are no other signs that anything is awry, I probably wouldn't worry about this. Is it possible that these non-canonical nucleotid…
Comment: Introducing NovaDemux, a Sequence Demultiplexer that Increases Yield and Salvage
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I examined nine 10B runs and five 25B runs (we just started getting 25B flowcells a few months ago). I did not pay close attention but it …
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by
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142k
> I don't know how widespread these issues are outside of JGI. Very few small/medium sequencing centers likely have NovaSeq X. This is the…
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by
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20k
Supporting figures, taken from some slides I prepared for internal use. HDist 0, 1, and 2 correspond to 0, 1, and 2 mismatches allowed in …
Comment: ComplexHeatmap - How to change fontsize of rowAnnotation
by
hannes.bongartz
• 0
This works. Thank you so so much!
Comment: Harmony integration group.by.var parameter
by
jared.andrews07
★ 17k
>I am not sure, but using "Sample_ID" might remove the differences between the conditions right ? More than likely, it'd at least impact t…
Comment: Harmony integration group.by.var parameter
by
Picasa
▴ 640
Thanks jared.andrews07 for your answer. So, you are suggesting to use only "Donor" in the integration? ```r RunHarmony(seu_obj, group.by.…
Comment: Single cell analysis: Unable to subset cells in seurat object using desired nFea
by
sc_analysis
• 0
I am not sure what should be the cut off. Looking at the vlnplot before subsetting i thought most of the cells are falling under 7500 nfeat…
Comment: How to calculate cell type frequency between two groups in single cell data
by
Sara
▴ 30
Thank you for your comment and sorry if this question might be so basic. How can I normalize the number of cells? If I am not wrong the i…
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