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194 results • Page
1 of 4
Sort: Views
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Views
Votes
Replies
6
votes
4
replies
13k
views
Trimmomatic: What is the difference between paired and unpaired output files in paired-end mode
trimmomatic
paired-end
updated 4 days ago by
Ruqaiya
• 0 • written 6.4 years ago by
dllopezr
▴ 130
9
votes
23
replies
9.8k
views
Tool:
BioLabDonkey - new Mac program for molecular biologists
software
biolabdonkey
4 days ago by
vytarasov
▴ 180
9
votes
7
replies
7.2k
views
Align paired and unpaired reads simultaneously using Bowtie2?
alignment
updated 4 days ago by
Ruqaiya
• 0 • written 5.7 years ago by
xiaozhongzhiping
▴ 20
2
votes
4
replies
5.6k
views
44% Successfully Assigned Fragments with featureCounts after 85% uniquely mapped reads with STAR
rna-seq
alignment
RNA-Seq
featureCounts
STAR
updated 2 days ago by
Thind amarinder
▴ 340 • written 4.9 years ago by
garbuzov
▴ 70
4
votes
12
replies
4.9k
views
10 follow
How to analyze Infinium Mouse Methylation BeadChip array data?
Methylation
Experience
Illumina
Mouse
updated 6 days ago by
Tawny
▴ 180 • written 3.0 years ago by
julia_geh
▴ 20
9
votes
13
replies
4.8k
views
Custom Reference panel creation for data imputation from .vcf files
imputation
reference panel
updated 5 days ago by
analyst
▴ 50 • written 6.4 years ago by
David_emir
▴ 490
0
votes
4
replies
3.6k
views
SNPEff database building error
SNP
updated 1 day ago by
Fungal genetics
• 0 • written 7.1 years ago by
ntyagi654
• 0
4
votes
4
replies
2.9k
views
CNVkit for somatic copy number detection
cnv
cnvkit
exome
WES
updated 1 day ago by
Anitha
• 0 • written 5.1 years ago by
stephaniem
• 0
2
votes
14
replies
2.9k
views
6 follow
CNVKIT - unable to produce scatter and diagram pdfs
cnvkit
updated 1 day ago by
Anitha
• 0 • written 4.8 years ago by
ww22runner
▴ 60
4
votes
3
replies
2.9k
views
How to solve the error 'ERROR::MATE_NOT_FOUND:Found xxx unpaired mates' when run SamToFastq
samtofasq
picard
validatesamfile
updated 6 days ago by
Pierre Lindenbaum
161k • written 6 days ago by
Lila M
★ 1.2k
6
votes
5
replies
2.3k
views
Tophat multiple or unique mapping criteria
RNA-Seq
updated 3 days ago by
Ruqaiya
• 0 • written 6.7 years ago by
maple964
• 0
0
votes
4
replies
2.3k
views
MGLTools does not work in windows 11
windows-11
Autodock
windows
MGLTools
updated 23 hours ago by
Ram
43k • written 13 months ago by
mohyeddine.taleb
• 0
0
votes
6
replies
2.1k
views
Copy number Variation analysis using conumee
R
software error
sequencing
genome
updated 6 days ago by
GenoMax
142k • written 3.2 years ago by
gs000095
▴ 10
1
vote
6
replies
1.8k
views
How can you *consistently* download BioProject IDs from NCBI's BioSample database using Entrez Direct?
entrez direct
ncbi
XML format
updated 13 minutes ago by
Ash
• 0 • written 3.9 years ago by
millere
• 0
5
votes
5
replies
1.7k
views
How to calculate coverage of Nanopore long read data?
sequencing
coverage
15 hours ago by
Arton
• 0
1
vote
2
replies
1.7k
views
Seurat CellCycleScoring – confused about the proper order of operations when using SCTransform
CellCycleScoring
SCTransform
Seurat
updated 6 days ago by
Li
• 0 • written 2.3 years ago by
GPM
▴ 10
1
vote
2
replies
1.3k
views
Extracting named fasta sequences according to list with Biopython
Biopython
FASTA
updated 1 day ago by
Rubayetul
• 0 • written 2.6 years ago by
lachiemck
• 0
1
vote
5
replies
726
views
Converting IGV to Figure using Illustrator (or other tools)
Illustrator
IGV
updated 2 hours ago by
Craft
• 0 • written 6 months ago by
Daniel
▴ 30
0
votes
3
replies
688
views
Pluritest for pluripotency broken
pluripotent
pluritest
cells
stem
updated 6 days ago by
DGTool
• 0 • written 21 months ago by
yassine
• 0
2
votes
9
replies
678
views
Filtering Multi-sample VCF file for all except one Genotype
variant
SNP
VCF
updated 12 hours ago by
Pierre Lindenbaum
161k • written 8 days ago by
schmince
• 0
0
votes
9
replies
567
views
Applying the metacell2 algorithm using python
python
single-cell
scanpy
metacell2
updated 5 hours ago by
Wayne
★ 2.0k • written 9 days ago by
JACKY
▴ 140
0
votes
3
replies
556
views
JASPAR2024_getMatrixSet error
JASPAR2024
getMatrixSet
updated 6 days ago by
Ram
43k • written 12 weeks ago by
maplewj
▴ 10
2
votes
5
replies
552
views
Tissue-specific DEG analysis with DEseq2
DEseq2
RNA-seq
DEG
R
updated 3 days ago by
arctic
▴ 40 • written 12 days ago by
M.
▴ 30
1
vote
7
replies
526
views
Question regarding WGCNA
WGCNA
Network-construction
6 days ago by
deepak
• 0
0
votes
11
replies
520
views
In IGV is this a heterogeneous mutation or false call?
mutations
IGV
heterogeneous
3 days ago by
Tuck898
• 0
0
votes
1
reply
513
views
Using samtools with GCS (google cloud storage) on a docker container seems to give "Protocol Not Supported" error
samtools
gcs
updated 2 days ago by
aw7
▴ 300 • written 25 days ago by
abhishekghadge
• 0
1
vote
4
replies
497
views
cellranger error message
multiplexing
cellranger
updated 5 days ago by
Max
• 0 • written 3 months ago by
Alivia
▴ 10
3
votes
6
replies
479
views
install package: package ‘gmwm’ is not available for this version of R
r
updated 2 days ago by
Pine
▴ 20 • written 10 days ago by
snajafy
• 0
2
votes
5
replies
475
views
featureCounts output summary assigned value higher than uniquely mapped reads from HISAT2
RNA-seq
featureCounts
HISAT
updated 23 hours ago by
Ram
43k • written 7 days ago by
Prawesh
• 0
4
votes
7
replies
462
views
RNA seq analysis
DESeq
RNA-seq
11 hours ago by
Jacek
▴ 10
1
vote
1
reply
448
views
ComBat_Seq stuck adjusting the data
Batch-Effect
RNAseq
ComBat-Seq
updated 6 days ago by
Jaïr
• 0 • written 4 months ago by
NorbertK
▴ 10
1
vote
2
replies
438
views
Pruning Phylogenetic Trees and Bootstrap Values
phylogenetics
bootstrap
6 days ago by
Zeng Hao
▴ 40
0
votes
8
replies
438
views
Different output for read length
samtools
BAM
6 days ago by
marco.barr
▴ 110
3
votes
3
replies
435
views
absolute path for symbolic links in Snakefile
Snakemake
updated 5 days ago by
Jesse
▴ 770 • written 26 days ago by
yifangt86
▴ 60
0
votes
2
replies
419
views
News:
FINAL CALL: 8th Berlin Summer School in NGS Data Analysis - Only a few last places available
DNA-seq
variant-calling
RNA-seq
illumina
transcriptomics
2 days ago by
David Langenberger
11k
1
vote
3
replies
419
views
Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read reverse strand" (flag 32/0x20)
PE
BAM
SAM
flag
paired-end
2 days ago by
kalavattam
▴ 190
0
votes
7
replies
409
views
RNAseq one control two conditions, shared and exclusive genes
conditions
RNAseq
multiple
Rstudio
updated 2 days ago by
Mohamed Abderrahmane
▴ 20 • written 16 days ago by
matteo.levorato
• 0
1
vote
4
replies
404
views
Can I readmap short reads to rDNA references?
rDNA
assembly
updated 5 days ago by
GenoMax
142k • written 28 days ago by
aniigodwinn
• 0
0
votes
6
replies
399
views
Is it Possible to "Merge" data from Replicates within a VCF SNP File
snp
vcf
genomics
plink
updated 1 day ago by
Ram
43k • written 4 days ago by
ajbarrett98
• 0
2
votes
5
replies
397
views
Importing a fastq file
Fastq
updated 5 days ago by
size_t
▴ 120 • written 6 days ago by
oumo
• 0
4
votes
7
replies
389
views
How does gene length effect the number of reads mapped
RNA-seq
CPM
sequencing
1 day ago by
Chen
• 0
0
votes
4
replies
381
views
Adata.raw.X in LIANA, something wrong with conversion from Seurat to adata in python.
k
2 days ago by
beginner123
• 0
1
vote
9
replies
380
views
UMI-Tools knee-method has great influence on the results of white list
single-cell
whitelist
UMI
RNA
UMI-Tools
updated 5 days ago by
i.sudbery
19k • written 8 days ago by
Assa Yeroslaviz
★ 1.8k
6
votes
7
replies
367
views
Longest transcript variant per gene
transcript
longest
variant
orthofinder
3 days ago by
sansan_96
▴ 90
1
vote
7
replies
364
views
Overlapping Ranges within Granges object
Genomicranges
IRanges
GRanges
16 hours ago by
ntsopoul
▴ 60
0
votes
1
reply
362
views
Snakemake fails to find conda in PBS
snakemake
updated 6 days ago by
tim.booth
▴ 60 • written 4 weeks ago by
yixinzeng
• 0
2
votes
6
replies
360
views
BWA alignment
Samtools
bam
updated 14 hours ago by
a.alnawfal.1992
▴ 260 • written 2 days ago by
Vahid
• 0
2
votes
8
replies
348
views
joint callset and vcf sorting, unknown TAG issue
sort
bcftools
GLNexus
merge
VCF
updated 3 days ago by
Pierre Lindenbaum
161k • written 3 days ago by
Matteo Ungaro
▴ 100
1
vote
2
replies
347
views
How to remove multiple batch effects from RNA-seq data before limma differential gene expression analysis?
ComBat-seq
limma
RNA-seq
removeBatchEffect
batch-effect
updated 2 days ago by
Ram
43k • written 3 days ago by
t.fortunato.asquini
• 0
0
votes
3
replies
344
views
Error in checkFullRank(modelMatrix) : the model matrix is not full rank, so the model cannot be fit as specified.
DESeq2
2 days ago by
mropri
▴ 150
194 results • Page
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Recent Votes
Answer: featureCounts output summary assigned value higher than uniquely mapped reads fr
Answer: Doubt about the process of annotation, detection, identification and classificat
Answer: Filtering VCF files based on VAF giving incorrect results
Problems with breakdancer (sv caller) output.
Problems with breakdancer (sv caller) output.
Comment: Add stats to boxplot in R
Answer: Genotyping sites with N in reference genome
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Recent Replies
Answer: How can you *consistently* download BioProject IDs from NCBI's BioSample databas
by
Ash
• 0
I know this was asked several years ago, but finding this post helped me solve my own problem and in the process I solved this one too. …
Answer: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i
by
swbarnes2
14k
Would Parkinson's be expected to have an effect on blood cells? Neurons, sure, but blood cells?
Comment: Doubt about the process of annotation, detection, identification and classificat
by
MarcosCosta
• 0
Thank you very much, you've helped me a lot
Comment: Filtering VCF files based on VAF giving incorrect results
by
Arton
• 0
Thank you. I can't try a completely new tool but I will keep your command for future use. Do you think my commands were correct? For exampl…
Comment: Add stats to boxplot in R
by
Ghada
• 0
Thanks. Thats work
Comment: Genotyping sites with N in reference genome
by
Zhenyu Zhang
★ 1.2k
Whether N -> A/T/G/C represents a variant is heavily dependent on the tool itself, and I know most tools don't. Since you don't see it in y…
Comment: calculating genomic coverage/ base overlap in R
by
marco.barr
▴ 110
The code seems correct at first glance... How did you generate the data in the Excel file? Have you checked the exon annotations? You could…
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
GenoMax
142k
Did you remeber to unzip/untar the `nt` preformatted files that you downloaded?
Comment: Genotyping sites with N in reference genome
by
shpak.max
▴ 50
Just to clarify, I have set additional sites in the reference genome to 'N', which means (based on your statement) that these will not be g…
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
Pierre Lindenbaum
161k
see https://www.biostars.org/p/9496674/ <!-- when i runed blastn(version 2.15) and nt database(latest version). I used aspera to download…
Answer: Genotyping sites with N in reference genome
by
Pierre Lindenbaum
161k
> Does UnifiedGenotyper skip/ignore sites where the reference genotype is N yes. The variant is in YOUR data, not in the reference where '…
Comment: Can 5' and 3' scRNAseq be processed with the same pipeline?
by
yura.grabovska
▴ 70
Do you have samples that should in theory have a good degree over overlap in terms of cell types across the two sequencing types? I would …
Comment: Wrong ZYGOSITY by ensembl vep on DeepVariant vcf file
by
asalimih
▴ 60
I found out that vep is only checking the GT field therefore assigning HOM to the Zygosity. Therefore the problem should be from deepvarian…
Comment: Add stats to boxplot in R
by
Lélé
▴ 10
To remove "Kruskall Wallis" you can try adding the argument label="p" or label = "p.signif" which displays the p-value or *** like this: …
Answer: Sequencing Depth (Read Depth) Calculations
by
GenoMax
142k
You could also use `pandepth` ([**LINK**][1]) or `mosdepth` ([**LINK**][2]) as well. These will allow you to be flexible about windowed cov…
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