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707 results • Page
3 of 15
Sort: replies
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Views
Votes
Replies
0
votes
6
replies
2.1k
views
Copy number Variation analysis using conumee
R
software error
sequencing
genome
updated 12 days ago by
GenoMax
142k • written 3.2 years ago by
gs000095
▴ 10
4
votes
5
replies
467
views
Figures are disappeared from html report of SnpEff
HTML
SnpEff
22 days ago by
analyst
▴ 50
3
votes
5
replies
423
views
Converting CRAM to FastQ
GATK
samtools
cram
SamToFastq
fastq
18 days ago by
Maverick
▴ 10
2
votes
5
replies
439
views
How to trim transcripts using information from NCBI contamination screen report
RNAseq
assembly
transcriptome
contamination
25 days ago by
Lada
▴ 30
3
votes
5
replies
383
views
Super ehancers
enhancers
updated 1 hour ago by
jared.andrews07
★ 17k • written 19 days ago by
Oburah
• 0
1
vote
5
replies
384
views
Reconstruction of locus, order contigs
contigs
cluster
alignment
locus
updated 4 days ago by
dthorbur
★ 2.0k • written 4 days ago by
BATMAN
• 0
4
votes
5
replies
3.4k
views
How to compare compare two Sam files to check mapping
alignment
updated 13 days ago by
DavidStreid
▴ 90 • written 6.4 years ago by
madhu.9124
▴ 60
0
votes
5
replies
353
views
Too many unpaired forward reads found by Trimmomatic
Trimmomatic
8 days ago by
SilhouetteQ
• 0
8
votes
5
replies
6.7k
views
ISCN annotation for SV/CN VCF files
SV
written 8 months ago by
a.beggs
▴ 60
0
votes
5
replies
456
views
Is it possible to get a list of representative genomes from a past RefSeq release?
representative
ncbi
asembly
refseq
updated 26 days ago by
GenoMax
142k • written 27 days ago by
Bertalan_Takacs
▴ 90
2
votes
5
replies
448
views
Importing a fastq file
Fastq
updated 11 days ago by
size_t
▴ 120 • written 12 days ago by
oumo
• 0
1
vote
5
replies
663
views
Cell barcode whitelists for DNBelab C Series High-throughput Single-cell RNA Series Library Preparation Set?
cell-barcode
MGI
DNBelab
single-cell
updated 28 days ago by
atowns21
• 0 • written 4 months ago by
benjamin.pyenson
• 0
3
votes
5
replies
365
views
Per base sequence content error in RNAseq analysis
RNA-seq
analysis
updated 29 days ago by
GenoMax
142k • written 29 days ago by
ahmad.sajad4541
• 0
6
votes
5
replies
2.3k
views
Tophat multiple or unique mapping criteria
RNA-Seq
updated 9 days ago by
Ruqaiya
• 0 • written 6.7 years ago by
maple964
• 0
6
votes
5
replies
1.7k
views
How to calculate coverage of Nanopore long read data?
sequencing
coverage
6 days ago by
Arton
▴ 10
2
votes
5
replies
556
views
Fetch table from clinvar database according to a list of rsid
python
clinvar
perl
23 days ago by
ashaneev07
▴ 20
5
votes
5
replies
354
views
Error in dimnames(x) <- dn : length of 'dimnames' [2] not equal to array extent
EGSEA
13 days ago by
Chris
▴ 280
1
vote
5
replies
512
views
How should I make kallisto indexes?
kallisto
updated 22 days ago by
dsull
★ 6.0k • written 5 weeks ago by
bioinfo
▴ 150
3
votes
5
replies
653
views
SLURM submission problem: Indexing the Reference Genome is not done in STAR
align
RNA-Seq
linux
STAR
29 days ago by
n_navy
• 0
1
vote
5
replies
814
views
Converting IGV to Figure using Illustrator (or other tools)
Illustrator
IGV
written 6 months ago by
Daniel
▴ 30
3
votes
5
replies
415
views
Practical Haplotype Graph v2 not finding correct paths
Pangenome
PHG
graph
6 hours ago by
beantkapoor16
▴ 10
1
vote
5
replies
414
views
Introduce SNPs on FASTA
simulation
snps
haplotypes
pharmacogenetics
18 days ago by
Riccardo
▴ 10
0
votes
5
replies
317
views
Percentage coverage of reference genome by de novo genome
SAMtools
BWA
alignment
ddRAD
updated 13 days ago by
Joe
21k • written 13 days ago by
Lemonhope
• 0
5
votes
5
replies
369
views
Problem with data downloaded from Short Reads Archive (SRA)
SRA
DADA2
metabarcoding
updated 19 days ago by
atharvakarkare14
▴ 40 • written 19 days ago by
Begonia_pavonina
▴ 150
0
votes
5
replies
223
views
Why most genes have high padj values
RNA-seq
DEG
updated 1 day ago by
Ram
44k • written 1 day ago by
mnx0723
• 0
1
vote
5
replies
488
views
HCL database download
HCL
updated 13 days ago by
Ram
44k • written 17 days ago by
sooni
▴ 20
4
votes
5
replies
4.4k
views
Tool:
GRIDSS: the Genomic Rearrangement IDentification Software Suite
Assembly
Structural-Variation
Variant-Calling
updated 4 days ago by
dario.garvan
▴ 520 • written 7.2 years ago by
d-cameron
★ 2.9k
0
votes
5
replies
461
views
6 follow
What purposes can TPM values be used for?
RNA-seq
TPM
Normalization
DESeq2
updated 18 days ago by
b.contreras.moreira
▴ 200 • written 19 days ago by
JH
• 0
0
votes
5
replies
364
views
A question about reference genome for creating the consensus sequence
fasta
fa
cram
genome
13 days ago by
me
• 0
0
votes
5
replies
441
views
Should I remove unlocalized scaffols in reference genome before alignment?
qualimap
alignment
minimap2
GRCh38
assembly
27 days ago by
njornet
▴ 20
0
votes
5
replies
319
views
calculating genomic coverage/ base overlap in R
genomics
updated 3 days ago by
1769mkc
★ 1.2k • written 6 days ago by
Xbox_27
• 0
1
vote
5
replies
415
views
Comparative Analysis of Algorithms for Implementing the FM-Index: Correctness, Complexity, and Use Cases
fmindex
bwt
updated 13 days ago by
Michael
54k • written 14 days ago by
qwertyuiop26
• 0
0
votes
5
replies
412
views
Easy way to find out which allele is minor allele from bed file?
plink
updated 17 days ago by
chrchang523
10k • written 18 days ago by
curious
▴ 750
2
votes
5
replies
963
views
Retrieval of Active site information programmatically
Catalytic
Python
Active
PDB
site
Site
updated 22 days ago by
Wayne
★ 2.0k • written 2.1 years ago by
arinjoy
• 0
2
votes
5
replies
292
views
What does the 'E%' represent in BUSCO results?
BUSCO
updated 7 days ago by
Philipp Bayer
8.5k • written 7 days ago by
林明德
• 0
1
vote
5
replies
284
views
Annotating file using bcftools
annotation
plink
bcftools
5 days ago by
kl
▴ 10
3
votes
5
replies
359
views
Generating mpileup file using samtools
mpileup
samtools
updated 18 days ago by
Joe
21k • written 18 days ago by
Ruqaiya
• 0
2
votes
5
replies
2.5k
views
identifying transgene insertion site in WGS
WGS
insertion site
trangene
de-novo
soap
updated 13 days ago by
Cameron.walker9900
• 0 • written 5.1 years ago by
Assa Yeroslaviz
★ 1.8k
2
votes
5
replies
524
views
featureCounts output summary assigned value higher than uniquely mapped reads from HISAT2
RNA-seq
featureCounts
HISAT
updated 6 days ago by
Ram
44k • written 13 days ago by
Prawesh
• 0
1
vote
5
replies
530
views
DYH17 Structure Prediction
BLAST
Protein-Structure-Prediction
28 days ago by
anasjamshed
▴ 120
2
votes
5
replies
561
views
Marking duplicates using UMIs
Deduplication
UMI
updated 29 days ago by
i.sudbery
19k • written 4 weeks ago by
Lipika
• 0
1
vote
5
replies
337
views
Can I perform a correlation test with 3 biological replicates per condition?
RNA-Seq
deseq2
updated 4 days ago by
Ram
44k • written 5 days ago by
manuelmourato25
• 0
1
vote
5
replies
387
views
install.packages("imudata") - package ‘imudata’ is not available for this version of R
r
updated 15 days ago by
Ram
44k • written 15 days ago by
snajafy
• 0
0
votes
5
replies
300
views
bbmerge (bbmap) ~ error with insert size file output
bbtools
bbmerge
bbmap
updated 5 days ago by
GenoMax
142k • written 6 days ago by
chrisk
• 0
0
votes
5
replies
364
views
How to convert normalized BigWig file to count matrix?
count-matrix
BigWig
8 days ago by
feather-W
• 0
0
votes
5
replies
474
views
different FeatureCounts output for the same data
fpkm
Counts
Rsubread
rna-seq
updated 24 days ago by
Istvan Albert
100k • written 27 days ago by
sehriban.buyukkilic
▴ 10
1
vote
5
replies
241
views
Circos Plot to Visualize Chromatin Accessibility Changes from ATAC-seq Data
circos
atacseq
updated 6 days ago by
Pierre Lindenbaum
161k • written 6 days ago by
biology_inform
▴ 50
0
votes
5
replies
345
views
how to treat the replicates while performing WGCNA
clustering
WGCNA
replicates
22 hours ago by
mavy
▴ 10
2
votes
5
replies
609
views
Tissue-specific DEG analysis with DEseq2
DEseq2
RNA-seq
DEG
R
updated 9 days ago by
arctic
▴ 40 • written 18 days ago by
M.
▴ 30
0
votes
5
replies
448
views
Telescope issue
Telescope
RNA-seq
updated 20 days ago by
GenoMax
142k • written 21 days ago by
eleven11
• 0
707 results • Page
3 of 15
Recent Votes
Answer: Question about samtools view flags (paired reads vs. properly paired reads)
Answer: Functional enrichment analysis for unique gene IDs
Comment: Super ehancers
Comment: Why does assigning genes with biomart give me different values than using a tran
Comment: Multiplexing for pooled CRISPR screen sequencing
Comment: How can we convert a vcf to fasta, so that I can blast some genes against that w
Answer: Differential Expression with limma: Contrast and Design Matrix, combinatorial ap
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Comment: Where are the illumina adapters on Trimmomatic take from?
by
bioinfo
▴ 150
Thank you for the explanation. Is it more appropriate then to use the sequence from the illumina documentation or the one from the github?
Comment: Question about samtools view flags (paired reads vs. properly paired reads)
by
mrk
• 0
Thanks. What determines what constitutes a good distance? I have some targeted panel data and looking at flagstat, almost all reads are pai…
Comment: Where are the illumina adapters on Trimmomatic take from?
by
GenoMax
142k
Illumina indexed adapters share a core sequence at the beginning which is what is indicated in your link. Trimming programs will remove seq…
Comment: Allele count of 2 for homoplasmic MT variants in VCF
by
Ram
44k
Please stop using `bioinformatics` as a tag unless your post is about the field of bioinformatics itself.
Comment: Multiplexing for pooled CRISPR screen sequencing
by
GenoMax
142k
> If we switch to pair-end sequencing with dual-index barcode You can use dual-indexing with single end reads. You don't need to do paired…
Comment: Super ehancers
by
jared.andrews07
★ 17k
That is not valid GFF format, so that is probably going to be problematic. See the [GFF spec](https://github.com/The-Sequence-Ontology/Spec…
Comment: Error in CIBERSORTx ($ operator is invalid for atomic vectors)
by
finch
• 0
Hey! I had this issue and I think I can help- could you take a screenshot of the .txt file opened in the notepad?
Answer: Is it possible to run CibersortX with a very small number of samples?
by
finch
• 0
Hi! I had a very similar issue and it's likely not at all related to your number of samples but to the format of your mixture file! Ciberso…
Comment: How can we convert a vcf to fasta, so that I can blast some genes against that w
by
Ram
44k
You cannot *convert* a VCF to a FASTA as they have vastly different information content. Please explain your process - what exactly do you …
Comment: Comparing Analysis Strategies for scRNA-seq Data: Separate vs. Merged Analysis o
by
Shukai
• 0
Thank you Ram for the comments! Noted for future posts.
Comment: Where to find old version of GATK best practice
by
Zhenyu Zhang
★ 1.2k
I am looking for diagrams, and step-by-step instructions of how to build my pipelines. I think the github is only helpful if you are a wdl…
Comment: Where to find old version of GATK best practice
by
Zhenyu Zhang
★ 1.2k
oh, this is helpful. Thanks.
Comment: Comparing Analysis Strategies for scRNA-seq Data: Separate vs. Merged Analysis o
by
Ram
44k
1. You can edit your post and add relevant details. There is no need to add separate comments with relevant details especially when it's no…
Answer: Functional enrichment analysis for unique gene IDs
by
geneontologyhelp
▴ 410
If you are looking at producing GO annotations, we currently only recommend InterProScan, and basically only for novel organisms or where t…
Answer: Question about samtools view flags (paired reads vs. properly paired reads)
by
Pierre Lindenbaum
161k
paired reads = the experiment was a paired end assay: each fragment was sequenced on 5' and 3', two FASTQ files are generated by the seque…
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