Deleted:Allele specific PCR amplification wrong or may work?

Question

[beshka194]

for allele specific PCR I designed the forward primers for amplification of normal allele and for mutant allele and reverse allele but what I did was I designed the primers in such a manner that the forward primer began like from the beginning of the reverse strand and the reverse primer began somewhere in the middle of the sequence and not at the end of the sequence. My question is will this cause a problem in amplification? since the sequence amplified was also small