Hi, I am studying gene expression studies with 30 samples with trinity.
*I successfully completed abundance_estimation using:
--samples_file --est_method RSEM **--output_dir (in brief here).
Then I got the following output folders with RSEM results- Abl_1, Abl_2, Abl_3, Bn_1, Bn_2, Bn_3,... map,etc in my current working directory.
*Now I want to Build Transcript and Gene Expression Matrices by RSEM ~/abundance_estimates_to_matrix.pl --est_method RSEM --gene_trans_map** .... then what more options?
I want to use option to count my already created RSEM output as input here (--basedire may be), and I am bit confused about what more options I need to add in this command. The instruction in wiki is confusing to me for this section. Thank you for your time.
It should be great if I have any assistance in my build up command using trinity package...
This could help