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116,738 results • Page
1 of 2335
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0
votes
1
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13
views
Raw counts using stringtie
stringtie
RNA-seq
analysis
counts
raw
16 minutes ago by
ahmad.sajad4541
• 0
0
votes
2
replies
42
views
Differential Expression Analysis using DESeq2 in R
Bioinformatics
analysis
R
deseq2
25 minutes ago by
Erina
• 0
0
votes
0
replies
8
views
Modify plot R mtDNA indel
plot
mtDNA
R
30 minutes ago by
marco.barr
▴ 90
0
votes
0
replies
11
views
Re-Import modified clusterProfiler's GSEA result from .csv for graphical output-generation (i.e. cnetplot, heatplot)
enrichplot
graphical_output
GSEA
clusterProfiler
40 minutes ago by
NorbertK
• 0
2
votes
2
replies
74
views
Analysis of intronic reads included scRNA-seq data
cell
single
updated 58 minutes ago by
t.montserrat.ayuso
▴ 10 • written 4 hours ago by
carolofharvest
▴ 30
7
votes
7
replies
327
views
High Malat-1 expression in single cell data
cell
analysis
single
updated 29 minutes ago by
dsull
★ 5.9k • written 4 days ago by
carolofharvest
▴ 30
0
votes
0
replies
17
views
WGCNA Module-Trait Heatmap
module_trait_heatmap
wgcna
heatmap
1 hour ago by
mja
• 0
0
votes
1
reply
42
views
Downloading older version of a tool
Alignment
tools
updated 1 hour ago by
GenoMax
142k • written 2 hours ago by
Ruqaiya
• 0
0
votes
0
replies
28
views
Generating illumina interops SAV like plots for multiqc reports on HPC
visualization
illumina
ngs
sequencing
3 hours ago by
rustykb
▴ 20
1
vote
1
reply
59
views
DSEQ2 analysis
DSEQ2
logfoldchane
updated 4 hours ago by
ATpoint
82k • written 4 hours ago by
adi.gershon1
• 0
795
votes
167
replies
142k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 3 months ago by
Biostar
2.7k • written 7.4 years ago by
Istvan Albert
100k
3
votes
5
replies
104
views
Generating mpileup file using samtools
mpileup
samtools
updated 1 hour ago by
Joe
21k • written 5 hours ago by
Ruqaiya
• 0
0
votes
5
replies
211
views
6 follow
What purposes can TPM values be used for?
RNA-seq
TPM
Normalization
DESeq2
updated 5 hours ago by
b.contreras.moreira
▴ 180 • written 23 hours ago by
JH
• 0
0
votes
3
replies
159
views
Does comparing two different groups to a common third group introduce bias in the analysis?
DESEQ
RNAseq
updated 6 hours ago by
ATpoint
82k • written 16 hours ago by
Qi
• 0
0
votes
0
replies
45
views
News:
Cellosaurus release 49 is available
cell
biocuration
line
database
news
knowledgebase
7 hours ago by
Amos Bairoch
▴ 120
0
votes
1
reply
73
views
Help with choosing a model species for Augustus for a de novo assembled genome.
augustus
annotation
assembly
genome
updated 1 hour ago by
dthorbur
★ 1.9k • written 7 hours ago by
Vijith
▴ 30
0
votes
0
replies
64
views
SIngle cell analysis
Seurat
scRNA-seq
Harmony
DoubletFinder
Merge
9 hours ago by
Jeyong
• 0
0
votes
2
replies
647
views
homer not configured properly
Homer
makeTagDirectory
updated 11 hours ago by
clairechung112
• 0 • written 23 months ago by
amahdi779
• 0
1
vote
3
replies
161
views
Bedtools merge minimum overlap?
bedtools
genomics
updated 11 hours ago by
Alex Reynolds
35k • written 18 hours ago by
SJP
• 0
0
votes
0
replies
60
views
Empty table plot using plotGseaTable()
FGSEA
16 hours ago by
Chris
▴ 260
0
votes
1
reply
106
views
VG : No reference-sense paths available in the graph; falling back to generic paths.
vg
updated 17 hours ago by
anovak
▴ 120 • written 21 hours ago by
Hang
• 0
1
vote
3
replies
153
views
DiffBind: no peaks in DBA
MACS2
DiffBind
ChIP-seq
updated 18 hours ago by
jared.andrews07
★ 16k • written 21 hours ago by
yvonneh
▴ 10
1
vote
2
replies
130
views
Converting CRAM to FastQ
GATK
samtools
cram
SamToFastq
fastq
updated 18 hours ago by
GenoMax
142k • written 18 hours ago by
Maverick
▴ 10
3
votes
8
replies
6.5k
views
7 follow
GATK genomicsDBimport intervals for WGS
gatk
genomicsdbimport
updated 19 hours ago by
Sd
• 0 • written 5.1 years ago by
Nicolas Rosewick
11k
0
votes
2
replies
116
views
Base Quality Score Recalibration with different reference genome
SNPs
GATK
BQSR
RNA-seq
19 hours ago by
dtnondorf
• 0
0
votes
1
reply
140
views
Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA2 - where to go from here?
nf-core
16S
amplicon
dada2
ampliseq
updated 11 hours ago by
Chris Dean
▴ 390 • written 20 hours ago by
sovrappensiero
▴ 90
1
vote
2
replies
148
views
RNA-seq data for deep learning classification
rna-seq
2 hours ago by
Yuju
• 0
2
votes
6
replies
258
views
How to access TCGA samples that were treated with a specific drug?
TCGA
RNA-seq
13 hours ago by
Qroid
▴ 40
0
votes
1
reply
464
views
Loss of 'var' using concatenation of AnnData objects
Scanpy
AnnData
updated 22 hours ago by
Hugo
• 0 • written 4 months ago by
Rachel
• 0
0
votes
1
reply
101
views
Provean help
variant
Provean
updated 19 hours ago by
Mensur Dlakic
★ 27k • written 22 hours ago by
Arun Sai Kumar
• 0
1
vote
1
reply
120
views
Download eQTL data of one specific gene for all tissues from GTEx
eQTL
GTEx
updated 22 hours ago by
bk11
★ 2.4k • written 1 day ago by
Jeol
▴ 20
1
vote
1
reply
128
views
Super ehancers
enhancers
updated 23 hours ago by
jared.andrews07
★ 16k • written 1 day ago by
Oburah
• 0
0
votes
5
replies
182
views
How does gene length effect the number of reads mapped
RNA-seq
CPM
sequencing
updated 1 hour ago by
i.sudbery
19k • written 1 day ago by
Chen
• 0
5
votes
5
replies
214
views
Problem with data downloaded from Short Reads Archive (SRA)
SRA
DADA2
metabarcoding
updated 1 day ago by
atharvakarkare14
▴ 30 • written 1 day ago by
Begonia_pavonina
▴ 150
1
vote
7
replies
380
views
Downsampling long-read BAM files
nanopore
BAM
QC
ONT
downsampling
3 hours ago by
eebloom
▴ 80
3
votes
3
replies
222
views
What analysis suitable to identify similarly expressed genes between two samples (treated vs untreated). Contrary to DGE analysis,
Gene-Expression
RNA
NOISeq
DGE
updated 4 hours ago by
ATpoint
82k • written 1 day ago by
alifafiq1
• 0
0
votes
0
replies
77
views
Use of annotation of integrated Seurat object in single sample
Seurat
1 day ago by
Bine
▴ 60
0
votes
0
replies
82
views
News:
Beyond beginner R: Advancing your coding skills - September 16-19
Advanced-Coding
Programming
R
updated 22 hours ago by
Ram
43k • written 1 day ago by
carlopecoraro2
★ 2.5k
0
votes
1
reply
199
views
Subsetting and merging back Seurat Object brings different results
Seurat
1 day ago by
Bine
▴ 60
0
votes
0
replies
104
views
Job:
Postdoctoral Position in Bioinformatics - Mainz, Germany
NGS
Genomics
Post-doc
R-loops
updated 22 hours ago by
Ram
43k • written 1 day ago by
4r-rtg
• 0
1
vote
1
reply
122
views
Discrepancy in read counts from fastq
downsampling
fastq
updated 22 hours ago by
Ram
43k • written 1 day ago by
marco.barr
▴ 90
0
votes
0
replies
74
views
edgeR contrasts for analysis of reciprocal hybrid crosses - averaging, pooling, and comparing groups
edgeR
1 day ago by
Rhiannon
• 0
0
votes
0
replies
85
views
Successfully identified and Masked rpeats using RM. What Next?
sequence
annotation
repeatmasker
illumina
assembly
1 day ago by
Vijith
▴ 30
9
votes
10
replies
440
views
6 follow
Bacterial plasmid analysis
bacteria
plasmid
wgs
hybridassembly
sequencing
updated 22 hours ago by
Zamin Iqbal
▴ 20 • written 1 day ago by
nicole.kavanagh
• 0
0
votes
1
reply
259
views
Is there a way to increase the automatic label text size in Cytoscape?
Cytoscape
updated 1 day ago by
Scooter
▴ 280 • written 7 days ago by
avocado123
• 0
0
votes
0
replies
86
views
Combining Methylation Microarray data from different platforms
Microarray
DNA
EPIC
450K
Methylation
1 day ago by
James
▴ 10
0
votes
1
reply
573
views
Basic stats in hierfstat
Fst
stats
hierfstat
updated 1 day ago by
mdav
• 0 • written 18 months ago by
Zoe
• 0
1
vote
3
replies
199
views
Odd alignment question/finding
Alignment
updated 4 hours ago by
barslmn
★ 2.1k • written 1 day ago by
poordumbsillyidiot
• 0
0
votes
1
reply
190
views
GATK won't produce figures while analyzing covariates to generate 'recal_plots.pdf'
GATK
recalibration
updated 1 day ago by
GenoMax
142k • written 1 day ago by
mgranada3
▴ 30
0
votes
0
replies
86
views
Tassel 5 GBS: GBSSeqToTagDBPlugin producing empty database
GBS
Tassel5
1 day ago by
meck
• 0
116,738 results • Page
1 of 2335
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Answer: High Malat-1 expression in single cell data
Understanding ATAC-seq data
High Malat-1 expression in single cell data
Answer: DSEQ2 analysis
High Malat-1 expression in single cell data
Convert vcf files with phased genotypes to standard haplotype format
Convert vcf files with phased genotypes to standard haplotype format
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Recent Replies
Comment: Raw counts using stringtie
by
ahmad.sajad4541
• 0
![stringtie error][1] [1]: /media/images/6475a009-e1a6-412b-8515-a8aec173
Answer: Differential Expression Analysis using DESeq2 in R
by
Erina
• 0
![The below image contains the code and result also.][1] [1]: /media/images/96562b96-d508-4f67-96d1-7c71aa17
Comment: High Malat-1 expression in single cell data
by
dsull
★ 5.9k
My response above was actually partly inspired off of a reading of your paper :)
Comment: Differential Expression Analysis using DESeq2 in R
by
ATpoint
82k
> everything is showing equal in environment section. But when I am checking for equalities, it’s showing False No idea what this means. P…
Answer: Analysis of intronic reads included scRNA-seq data
by
t.montserrat.ayuso
▴ 10
You can calculate the percentage of intronic reads for each cell using the [DropletQC][1] package for R. In this preprint we discuss th…
Comment: How does gene length effect the number of reads mapped
by
i.sudbery
19k
I did say almost - obviously things are going to get tricky at gene ends - this is why we have the concept of effective length. Its interst…
Answer: High Malat-1 expression in single cell data
by
t.montserrat.ayuso
▴ 10
Malat1 correlates with the intronic content and can be used as a nuclear indicator. In this preprint we discuss about this artifact and the…
Comment: How does gene length effect the number of reads mapped
by
dsull
★ 5.9k
I wouldn't say it's true that number of reads is linearly proportional to the length of the gene. That's just an assumption that people mak…
Comment: Help with choosing a model species for Augustus for a de novo assembled genome.
by
dthorbur
★ 1.9k
Using any of the species present would likely result in a resonable annotation set. You could use *Arabidopsis* since it likely has the mos…
Comment: Downloading older version of a tool
by
GenoMax
142k
Have you tried conda install <pkg>=<version> so conda install smalt=0.5.8 <!-- junk -->
Comment: Generating mpileup file using samtools
by
Joe
21k
Older versions of software are usually available via distribution tools or the websites. It may require a lot of digging, but its almost ce…
Comment: RNA-seq data for deep learning classification
by
Yuju
• 0
Thank you very much for sharing your advice. Yes, it definitely makes sense that with the use of deep learning, models would learn normalis…
Comment: Generating mpileup file using samtools
by
Ruqaiya
• 0
I didn't use the same tool as in the paper...
Comment: read length in structural variant calling
by
eebloom
▴ 80
Yes good idea. I guess the quality of the SV calls and as a proxy for quality the length and distribution of variants called might be infor…
Comment: Generating mpileup file using samtools
by
Ruqaiya
• 0
I just realised I didn't align my reads with the tools they used and used bowtie2 instead. I can't download the older version that is menti…
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