Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Limit : all time
all time
today
this week
this month
this year
117,239 results • Page
4 of 2345
Sort: Rank
Rank
Views
Votes
Replies
1
vote
4
replies
351
views
Single cell analysis: Unable to subset cells in seurat object using desired nFeature_RNA cut off
single-cell
8 days ago by
sc_analysis
• 0
1
vote
2
replies
250
views
Overlapping clusters for different biological conditions: Seurat, UMAP
Seurat
scRNA-seq
UMAP
1 day ago by
bio_info
▴ 20
3
votes
4
replies
532
views
Single-cell ambient RNA correction: SoupX vs decontX contamination fraction
single-cell
ambient-RNA
updated 8 days ago by
fracarb8
★ 1.7k • written 16 days ago by
txema.heredia
▴ 130
0
votes
0
replies
182
views
R code for KEGG pathways output
KEGGREST
dplyr
KEGG
R
8 days ago by
doramora
▴ 10
0
votes
1
reply
184
views
finding highly divergent orthologs using exonerate
ortholog
Exonerate
evolution
updated 4 days ago by
dthorbur
★ 2.0k • written 8 days ago by
Emily
▴ 20
2
votes
3
replies
293
views
How to calculate nucleotide diversity (mtDNA, PacBio_data), Suggest me some tools
NucleotideDiversity
mtDNA
PacBio
8 days ago by
hashim.rana11
▴ 20
0
votes
1
reply
200
views
Does prokka work the same on forward and reverse complementary reads?
Prokka
updated 8 days ago by
Pierre Lindenbaum
162k • written 8 days ago by
Maksim
• 0
1
vote
2
replies
309
views
Trouble with PLINK's logistic regression analysis and covariatesTrouble with PLINK's logistic regression analysis and covariates
PLINK
GWAS
SNP
8 days ago by
F110152169
• 0
8
votes
10
replies
3.2k
views
8 follow
Venter Genome Vcf
vcf
updated 5 days ago by
Jeremy Leipzig
22k • written 10.2 years ago by
win
▴ 980
0
votes
0
replies
171
views
16S analysis for specie of bacteria identification
Species-identification
16S
Kraken2
updated 8 days ago by
Ram
44k • written 9 days ago by
Christopher
▴ 10
0
votes
0
replies
151
views
abundance_estimates_to_matrix.pl in Trinity is not generating any matrices / output
trinity
updated 9 days ago by
GenoMax
142k • written 9 days ago by
jway
• 0
0
votes
3
replies
430
views
What is a good way to do gene differentials in single cell data where one group is small 90 cells and the other group 30,000 cells or 2000 cells.
cell
RNA-Seq
single
updated 8 days ago by
ATpoint
83k • written 9 days ago by
shahzaibali
• 0
0
votes
2
replies
254
views
Trying to understand warning from MACS2 about too few paired peaks and differing results in DiffBind
MACS2
ATACseq
DiffBind
3 days ago by
atan
• 0
0
votes
2
replies
269
views
What do the transcript variant # mean in RefSeq?
refseq
updated 9 days ago by
Ram
44k • written 9 days ago by
curious
▴ 750
4
votes
6
replies
468
views
Mouse or Rat Gene Expression Data Similar to GTEx
whole-body
expression
atlas
9 days ago by
Shicheng Guo
★ 9.4k
1
vote
3
replies
344
views
Error when looping over multiple columns in a data frame in R
Statistics
R
updated 9 days ago by
Jeremy
▴ 910 • written 9 days ago by
Mohamed Samir
▴ 30
2
votes
0
replies
265
views
Job:
Permanent Position as Researcher/Computational Biologist at the National Genomics Infrastructure (Sweden)
Genomics
Sequencing
Sweden
Stockholm
SciLifeLab
9 days ago by
Matthias Zepper
4.6k
0
votes
1
reply
191
views
BEAST2.5 Chloroplast Divergence Time using Fossil Records
BEAST2.5
Fossil
updated 9 days ago by
Ram
44k • written 9 days ago by
a.bibek52
▴ 10
3
votes
7
replies
4.0k
views
Difference in Bismark output methylation call files and coverage files
RRBS
Bismark
DNA-methylation
updated 9 days ago by
Papyrus
★ 2.9k • written 3.9 years ago by
linelr
▴ 40
3
votes
2
replies
346
views
Job:
2 PhD Students in single-cell bioinformatics
PhD
Genomics
single-cell
NGS
3 days ago by
mtabaka
▴ 30
0
votes
1
reply
191
views
minfi::getQC - badsamplecutoff 10.5
getqc
minfi
methylation
EPICv2
updated 9 days ago by
Papyrus
★ 2.9k • written 9 days ago by
June
• 0
0
votes
2
replies
699
views
minfi::getQC - is default badsamplecutoff of 10.5 always appropriate? ~half of samples fail by this measure
getqc
ewas
minfi
updated 9 days ago by
June
• 0 • written 10 months ago by
rkb965
• 0
4
votes
6
replies
365
views
Error with BiocParallel. No barcodes files found
Barcodes
scRNA-seq
SingleCellExperiment
8 days ago by
bio_info
▴ 20
3
votes
3
replies
250
views
Unexpected read length from NGS
NGS
Illumina
8 days ago by
QX
▴ 10
2
votes
7
replies
427
views
samtools write-index
samtools
5 days ago by
R.L.
• 0
2
votes
3
replies
337
views
How do we do quantification using stringtie merge option for all the merged samples generated.
stringtie
quantification
5 days ago by
Varsha
• 0
3
votes
2
replies
196
views
How to interpret this plotMDS of three disease clusters?
microarray
plotMDS
DEGs
updated 9 days ago by
ATpoint
83k • written 9 days ago by
egascon
• 0
0
votes
0
replies
101
views
GWAS or QTL mapping in RIL population
GWAS
RIL
9 days ago by
韩雨
• 0
0
votes
0
replies
104
views
VEP annotation tool: "." isn't numeric in addition (+)
VEP
VCF
updated 9 days ago by
Ram
44k • written 9 days ago by
emmanouil.a
▴ 130
11
votes
13
replies
6.8k
views
7 follow
log2 fold change in RNA-seq analysis
fold
log2
change
updated 9 days ago by
May Ling
▴ 10 • written 2.4 years ago by
wmsalsah
▴ 10
0
votes
1
reply
278
views
GWAS Phenotypes
GWAS
updated 9 days ago by
韩雨
• 0 • written 6 weeks ago by
solomoncharles77
▴ 90
5
votes
4
replies
5.2k
views
6 follow
Classic threshold for log2 fold change in RNA-seq experiment
RNA-Seq
updated 9 days ago by
May Ling
▴ 10 • written 3.9 years ago by
Mozart
▴ 330
1
vote
1
reply
150
views
Cellranger-multi : Demultiplexing and Analyzing 5’ Immune Profiling Libraries Pooled with Hashtags
5InmuneProfiling
nf-core
scRNAseq
cellranger-multi
Nextflow
updated 9 days ago by
GenoMax
142k • written 9 days ago by
azeu
▴ 10
0
votes
0
replies
73
views
Assigning Micro-C read pairs to nucleosomes called from MNase-seq data?
Hi-C
MNase-Seq
9 days ago by
Linus
• 0
7
votes
3
replies
13k
views
Z score in RNAseq
RNA-Seq
Zscore
updated 9 days ago by
Akash D
▴ 60 • written 6.5 years ago by
rob.costa1234
▴ 310
0
votes
1
reply
164
views
Free AI for R programming
R
updated 9 days ago by
Ram
44k • written 9 days ago by
mohamadzare6022
▴ 10
9
votes
13
replies
876
views
7 follow
Forum:
Ideal PC configurations and operating system for bioinformatics laboratory
PC
updated 9 days ago by
i.sudbery
19k • written 5 weeks ago by
Estevão
▴ 10
0
votes
0
replies
109
views
Extracting haplotype-specific annotations from splicing graph
vg
9 days ago by
Juhyun
• 0
0
votes
0
replies
104
views
miloR - object 'as.SimpleList' of mode 'function' was not found when running calcNhoodDistance
scRNA-seq
seurat
miloR
9 days ago by
shuaizh117
▴ 10
0
votes
0
replies
105
views
Phyloseq returning black bars for plot_bar
phyloseq
bioconductor
R
9 days ago by
Jacob
• 0
3
votes
8
replies
899
views
Random Access remote BAM files
htslib
BAM
updated 9 days ago by
a.penatauber
• 0 • written 3 months ago by
Lucas R.F.
▴ 20
0
votes
0
replies
104
views
Cancer vs Normal Cell Classification Based on SNVs
cancer
10 days ago by
dglad
• 0
0
votes
4
replies
248
views
merge overlaps and remove non overlap genomic windows
BED
intersection
overlap
9 days ago by
reza
▴ 300
0
votes
1
reply
162
views
Small secondary peak for per sequence GC content - FASTQC results (bulk RNA-seq)
RNAseq
updated 10 days ago by
GenoMax
142k • written 10 days ago by
Fossil
• 0
1
vote
2
replies
265
views
Construction of circos plot from WGS data
WGS
Circos
8 days ago by
Anitha
▴ 10
1
vote
3
replies
258
views
Trimmomatic running but files containing purged reads are empty
fastqc
fastq
trimmomatic
updated 10 days ago by
GenoMax
142k • written 10 days ago by
Wilber0x
▴ 50
0
votes
0
replies
113
views
How to find nucleotide changes and the predicted effect when comparing two genomes
BLAST
alignment
updated 10 days ago by
Ram
44k • written 10 days ago by
Melissa
• 0
5
votes
3
replies
227
views
FastQC interpretation - 16S sequencing
interpretation
amplicon
Fastqc
16S
updated 10 days ago by
GenoMax
142k • written 10 days ago by
Christopher
▴ 10
0
votes
0
replies
132
views
Subset of ATAC-seq peaks has high variance
atac-seq
edgeR
R
normalization
9 days ago by
Tim
• 0
2
votes
6
replies
350
views
How to get the ratio of allele counts from GATK derived VCF file?
Freebayes
GATK
SNP
VCF
9 days ago by
anikcropscience
▴ 230
117,239 results • Page
4 of 2345
Recent Votes
Answer: Mailing list for academic programs and positions
A: Nextflow using existing Conda Environments
Answer: DESeq2 model matrix not full rank
Answer: How do you make an original contribution to knowledge in applied bioinformatics?
Comment: How to determine number of SNPs called?
Answer: NCBI vs Ensembl - Ortholog genes Information
Answer: Set the same Y scale for different plots
Recent Locations •
All
Spain,
6 minutes ago
Whitefish, MT,
9 minutes ago
France,
10 minutes ago
United States,
16 minutes ago
Belgium,
21 minutes ago
France,
23 minutes ago
Germany,
32 minutes ago
Recent Awards •
All
Popular Question
to
citronxu
▴ 20
Commentator
to
Istvan Albert
100k
Popular Question
to
Mark
• 0
Popular Question
to
mgdias.jose
▴ 20
Popular Question
to
Shred
★ 1.4k
Popular Question
to
arriyaz.nstu
▴ 30
Popular Question
to
Fred Hutch (Recruiting)
▴ 20
Recent Replies
Comment: How do you make an original contribution to knowledge in applied bioinformatics?
by
Edna
• 0
Thank you, this was really helpful and clarifying for me. While I couldn't find anybody comfortable mentoring me in bioinformatics in the b…
Comment: Retrive amino acids sequences from NCBI E-Direct
by
Patadu94
• 0
I found this old [post][1] and I was able to retrive a single amino acid sequence for the Gene_ID (Locus_Tag) that I include in the `-query…
Comment: I got qlen = 276 using bam_cigar2qlen in htslib but I count that cigar by myself
by
Aaron
▴ 10
my encoding logic is as following: const static std::unordered_map<int, int> opMapping { {'M', 0}, {'I', 1}, {'D', 2}, …
Comment: interpreting hisat2 alignment summary from paired end reads
by
GenoMax
142k
30821396 (82.71%) aligned concordantly exactly 1 time --> Uniquely mapped 2329205 (6.25%) aligned concordantly >1 times --> Multi-mapped
Answer: How do you make an original contribution to knowledge in applied bioinformatics?
by
i.sudbery
19k
If we modify the question to be "How do we make a contribution in applied molecular biology?" we would think this was a very odd question. …
Comment: Set the same Y scale for different plots
by
G.S
▴ 10
Thanks very much for your help! This code works and solves the problem. I really appreciate you going the extra mile and adding a code expl…
Comment: Filtering VCF - minimal valid filter parametrs
by
Lukas
▴ 50
Thank you so much. I think this is literally what i was looking for. You are lifesaver.
Comment: How to calculate cell type frequency between two groups in single cell data
by
Francesco
▴ 20
For me scProportionTest library does the trick.
Answer: How do you make an original contribution to knowledge in applied bioinformatics?
by
noodle
▴ 590
I'm 10 years in the field of 'molecular biology', which could be considered early-mid career. My opinion is that these days you need profic…
Comment: RNA Seq Paired End High Duplication Rate
by
Tara
• 0
Thank you! repair.sh worked great!
Comment: Posts getting removed?
by
noodle
▴ 590
> Was this example your post or a post you had posted in? I don't recall. It's only for an example here as I noticed that it was deleted a…
Comment: Posts getting removed?
by
GenoMax
142k
I am going to tag https://www.biostars.org/u/2/ (site owner) since he is the only person who has the means of tracking things on database e…
Comment: Posts getting removed?
by
noodle
▴ 590
> Give us examples. It's hard to search deleted posts. Was this one removed by mods or the user (possibly me)? https://www.biostars.org/p…
Comment: Posts getting removed?
by
Ram
44k
> users should be forced to educate themselves before being allowed to post How I wish this could be enforced - we used to have a mechanis…
Comment: Posts getting removed?
by
Mensur Dlakic
★ 27k
> I've written a few how-to posts that are aimed to educate users. New users rarely invest time in reading before creating a new post so th…
Traffic: 1156 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6