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49 results • Page
1 of 1
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0
votes
0
replies
15
views
Quetion regarding WGCNA
WGCNA
Network
construction
1 hour ago by
deepak180001
• 0
0
votes
0
replies
17
views
Design matrix Differential expression analysis
expression
designmatrix
analysis
Differential
RNA-sequence
1 hour ago by
SHN
▴ 40
0
votes
1
reply
50
views
visualize GSEA
enrichment
barplot
RNA-seq
GSEA
positive
updated 1 hour ago by
bk11
★ 2.4k • written 3 hours ago by
Rob
▴ 170
0
votes
0
replies
36
views
fastq screen aligner no specified.
BWA
Bowtie2
FastqScreen
3 hours ago by
Ximena
• 0
0
votes
0
replies
30
views
filtering before dada2
metagenomic
bbduk
qiime2
dada2
bioinformatics
3 hours ago by
Valentina
• 0
0
votes
6
replies
229
views
Differential Expression Analysis using DESeq2 in R
deseq2
R
updated 2 hours ago by
swbarnes2
14k • written 10 hours ago by
Erina
• 0
0
votes
1
reply
59
views
Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read reverse strand" (flag 32/0x20)
PE
BAM
SAM
flag
paired-end
updated 3 hours ago by
swbarnes2
14k • written 3 hours ago by
kalavattam
▴ 190
0
votes
1
reply
60
views
TaxID mapping file
annotation
blobtools
RNAseq
decontamination
transcriptomes
updated 5 hours ago by
GenoMax
142k • written 5 hours ago by
Lada
▴ 30
0
votes
0
replies
47
views
.lgen file has 3+ different alleles
PLINK
lgen
ped
6 hours ago by
Emilie
• 0
0
votes
4
replies
140
views
Easy way to find out which allele is minor allele from bed file?
plink
5 hours ago by
curious
▴ 750
0
votes
0
replies
46
views
vcf procesing for ML antigen prediction
formats
vcf
fastaq
HLA_imputation
HLA_typing
7 hours ago by
Javier
• 0
0
votes
0
replies
39
views
Extracting mutation status from MAF files - TCGA
TCGA
maf
mutation
8 hours ago by
yahn
• 0
0
votes
0
replies
47
views
IGV interpretation for ATM gene for chromosome 11 for a deceased partient suspected of having melanoma
genetic
Variant
predisposition
8 hours ago by
mikemakaveli1
• 0
0
votes
0
replies
47
views
Tissue-specific DEG analysis with DEseq2
DEseq2
RNA-seq
DEG
R
updated 7 hours ago by
Ram
43k • written 8 hours ago by
M.
▴ 30
0
votes
2
replies
101
views
Raw counts using stringtie
stringtie
RNA-seq
updated 7 hours ago by
GenoMax
142k • written 9 hours ago by
ahmad.sajad4541
• 0
0
votes
0
replies
44
views
Modify plot R mtDNA indel
plot
mtDNA
R
9 hours ago by
marco.barr
▴ 90
0
votes
0
replies
48
views
Re-Import modified clusterProfiler's GSEA result from .csv for graphical output-generation (i.e. cnetplot, heatplot)
enrichplot
graphical_output
GSEA
clusterProfiler
9 hours ago by
NorbertK
• 0
2
votes
2
replies
123
views
Analysis of intronic reads included scRNA-seq data
cell
single
updated 10 hours ago by
t.montserrat.ayuso
▴ 10 • written 13 hours ago by
carolofharvest
▴ 30
9
votes
9
replies
445
views
High Malat-1 expression in single cell data
cell
analysis
single
updated 7 hours ago by
dsull
★ 6.0k • written 4 days ago by
carolofharvest
▴ 30
0
votes
1
reply
88
views
Downloading older version of a tool
Alignment
tools
updated 10 hours ago by
GenoMax
142k • written 11 hours ago by
Ruqaiya
• 0
0
votes
0
replies
59
views
Generating illumina interops SAV like plots for multiqc reports on HPC
visualization
illumina
ngs
sequencing
12 hours ago by
rustykb
▴ 20
1
vote
1
reply
82
views
DSEQ2 analysis
DSEQ2
logfoldchane
updated 13 hours ago by
ATpoint
82k • written 13 hours ago by
adi.gershon1
• 0
3
votes
5
replies
175
views
Generating mpileup file using samtools
mpileup
samtools
updated 10 hours ago by
Joe
21k • written 14 hours ago by
Ruqaiya
• 0
0
votes
5
replies
240
views
6 follow
What purposes can TPM values be used for?
RNA-seq
TPM
Normalization
DESeq2
updated 15 hours ago by
b.contreras.moreira
▴ 180 • written 1 day ago by
JH
• 0
1
vote
3
replies
195
views
Does comparing two different groups to a common third group introduce bias in the analysis?
DESEQ
RNAseq
updated 15 hours ago by
ATpoint
82k • written 1 day ago by
Qi
• 0
0
votes
0
replies
59
views
News:
Cellosaurus release 49 is available
cell
biocuration
line
database
news
knowledgebase
16 hours ago by
Amos Bairoch
▴ 120
1
vote
3
replies
170
views
Help with choosing a model species for Augustus for a de novo assembled genome.
augustus
annotation
assembly
genome
updated 8 hours ago by
dthorbur
★ 1.9k • written 16 hours ago by
Vijith
▴ 30
0
votes
0
replies
79
views
SIngle cell analysis
Seurat
scRNA-seq
Harmony
DoubletFinder
Merge
18 hours ago by
Jeyong
• 0
0
votes
2
replies
666
views
homer not configured properly
Homer
makeTagDirectory
updated 20 hours ago by
clairechung112
• 0 • written 23 months ago by
amahdi779
• 0
1
vote
3
replies
179
views
Bedtools merge minimum overlap?
bedtools
genomics
updated 20 hours ago by
Alex Reynolds
35k • written 1 day ago by
SJP
• 0
0
votes
0
replies
76
views
Empty table plot using plotGseaTable()
FGSEA
2 hours ago by
Chris
▴ 260
3
votes
5
replies
213
views
Converting CRAM to FastQ
GATK
samtools
cram
SamToFastq
fastq
5 hours ago by
Maverick
▴ 10
0
votes
1
reply
161
views
Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA2 - where to go from here?
nf-core
16S
amplicon
dada2
ampliseq
updated 20 hours ago by
Chris Dean
▴ 390 • written 1 day ago by
sovrappensiero
▴ 90
1
vote
2
replies
183
views
RNA-seq data for deep learning classification
rna-seq
11 hours ago by
yahn
• 0
2
votes
6
replies
286
views
How to access TCGA samples that were treated with a specific drug?
TCGA
RNA-seq
22 hours ago by
Qroid
▴ 40
0
votes
6
replies
251
views
How does gene length effect the number of reads mapped
RNA-seq
CPM
sequencing
updated 8 hours ago by
dsull
★ 6.0k • written 1 day ago by
Chen
• 0
1
vote
7
replies
414
views
Downsampling long-read BAM files
nanopore
BAM
QC
ONT
downsampling
12 hours ago by
eebloom
▴ 80
3
votes
3
replies
273
views
What analysis suitable to identify similarly expressed genes between two samples (treated vs untreated). Contrary to DGE analysis,
Gene-Expression
RNA
NOISeq
DGE
updated 13 hours ago by
ATpoint
82k • written 1 day ago by
alifafiq1
• 0
1
vote
3
replies
211
views
Odd alignment question/finding
Alignment
updated 13 hours ago by
barslmn
★ 2.1k • written 1 day ago by
poordumbsillyidiot
• 0
0
votes
7
replies
501
views
Post-imputation plot
michigan-imputation-server
quality-control
updated 22 hours ago by
LChart
3.9k • written 4 days ago by
kl
▴ 10
1
vote
5
replies
301
views
Introduce SNPs on FASTA
simulation
snps
haplotypes
pharmacogenetics
14 hours ago by
Riccardo
▴ 10
0
votes
5
replies
284
views
RNAseq one control two conditions, shared and exclusive genes
conditions
RNAseq
multiple
Rstudio
updated 15 hours ago by
Mohamed Abderrahmane
▴ 20 • written 4 days ago by
matteo.levorato
• 0
5
votes
5
replies
503
views
Tutorial:
how to combine multiple RNAseq count files into a single dataframe in R and unix
Unix
RNAseq
R
updated 4 hours ago by
Mbofire
• 0 • written 8 days ago by
Ming Tommy Tang
★ 3.9k
1
vote
6
replies
320
views
Do I need to go back and filter my long-reads?
alignment
nanopore
filtering
QC
ONT
updated 7 hours ago by
GenoMax
142k • written 18 days ago by
eebloom
▴ 80
1
vote
2
replies
275
views
read length in structural variant calling
nanopore
SV
QC
ONT
variant
12 hours ago by
eebloom
▴ 80
4
votes
9
replies
2.1k
views
6 follow
Same sequencing sample in multiple lanes. How to analyse it?
sequencing
bowtie2
alignment
samtools
ngs
updated 8 hours ago by
ST
• 0 • written 2.2 years ago by
Federico
• 0
0
votes
1
reply
542
views
HOMER on AWS
HOMER
updated 20 hours ago by
clairechung112
• 0 • written 2.2 years ago by
Bogdan
★ 1.4k
16
votes
12
replies
7.3k
views
10 follow
how to split multi-fasta file into single fasta file named by header
genome
perl
python3
bash
python
updated 14 hours ago by
rsieber
▴ 10 • written 3.1 years ago by
Kumar
▴ 120
2
votes
2
replies
1.7k
views
Parametric or non-parametric tests for qPCR significance testing between treatments?
qPCR
statistical test
parametric
non-parametric
updated 5 hours ago by
e.r.zakiev
▴ 210 • written 3.8 years ago by
n.anuragsharma
▴ 40
49 results • Page
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Recent Votes
Comment: No INFO/info value in headers
how to combine multiple RNAseq count files into a single dataframe in R and unix
Comment: Greatly speed up conda by using mamba
Greatly speed up conda by using mamba
Comment: Converting CRAM to FastQ
Parametric or non-parametric tests for qPCR significance testing between treatments?
Answer: Converting CRAM to FastQ
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SHN
▴ 40
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Rob
▴ 170
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to
GenoMax
142k
Popular Question
to
curious
▴ 750
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M.
▴ 30
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Answer: visualize GSEA
by
bk11
★ 2.4k
I have made some edits in your code basically changing the position of text and commented out the line `data$text_pos <- ifelse(data$NES > …
Comment: Differential Expression Analysis using DESeq2 in R
by
swbarnes2
14k
Pictures of code are useless. You were told to paste the code and use the 0101 button. Can you explain why you decided not to do that? If…
Comment: Differential Expression Analysis using DESeq2 in R
by
Erina
• 0
![Here is the code I am using for the analysis][1] [1]: /media/images/8a511904-6ae8-47f5-b647-ee15b02b
Answer: Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read
by
swbarnes2
14k
In paired end sequencing, every read has a mate, and the flags all together tell you not only what is up with the read you are looking at, …
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
by
Mbofire
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Comment: Easy way to find out which allele is minor allele from bed file?
by
curious
▴ 750
According to the link: "When *generating* such filesets, PLINK 1.x defaults to swapping the alleles whenever A1's frequency is above (not …
Comment: TaxID mapping file
by
GenoMax
142k
nodesDB file should have been installed if you had used "Install" script for `blobtools` according to : https://blobtools.readme.io/docs/ta…
Comment: Parametric or non-parametric tests for qPCR significance testing between treatme
by
e.r.zakiev
▴ 210
obviously if the values are really sampled from a (quasi) normally-distributed values, then n doesn't matter, he could use t-test even with…
Comment: Converting CRAM to FastQ
by
Maverick
▴ 10
Understood Thank you!
Comment: Converting CRAM to FastQ
by
GenoMax
142k
You can use any reference that you like at this point. Since you are planning to use GATK, you can get their version from the resource bund…
Comment: Converting CRAM to FastQ
by
Maverick
▴ 10
![enter image description here][1]I was able to get my fastq files. I can see that my reference files are cached in a hts-ref directory but…
Comment: Differential Expression Analysis using DESeq2 in R
by
swbarnes2
14k
Okay, that shows us the problem, but not what we need to solve it. But, for instance, are you sure that period after the sample name shou…
Comment: Easy way to find out which allele is minor allele from bed file?
by
bk11
★ 2.4k
In the case of MAF = 0.5, I think A1 is still the minor allele. You can check the discussion in this [link][1]. [1]: https://groups.goo…
Comment: Easy way to find out which allele is minor allele from bed file?
by
curious
▴ 750
Yes, but I think the problem is what to do when MAF = 0.5
Answer: Easy way to find out which allele is minor allele from bed file?
by
bk11
★ 2.4k
In Plink, A1 is usually a minor allele and A2 a major allele. .frq (basic allele frequency report) Produced by --freq. …
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