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179 results • Page
3 of 4
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Votes
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0
votes
0
replies
61
views
How to find nucleotide changes and the predicted effect when comparing two genomes
BLAST
alignment
updated 9 hours ago by
Ram
44k • written 11 hours ago by
Melissa
• 0
0
votes
0
replies
151
views
qPCR gene expression data analysis
qPCR
geneexpression
anova
6 days ago by
ebertomeup
• 0
0
votes
0
replies
152
views
Blog:
The Current State and Future Prospects of Long-Read Sequencing
LRS
6 days ago by
Novogene
▴ 420
0
votes
1
reply
187
views
What's next after GATK variant calling pipeline?
GATK
pipeline
figures
DNA-seq
updated 6 days ago by
Michael
54k • written 7 days ago by
mgranada3
▴ 30
0
votes
1
reply
191
views
perseus software (version 1.5.5)
preseus
mass
spectrometry
data
updated 6 days ago by
DGTool
▴ 20 • written 8 days ago by
iqra
• 0
0
votes
1
reply
173
views
Seurat v5 and how to correctly integrate across multiple experiments
Seurat
scRNA
R
updated 6 days ago by
Papyrus
★ 2.9k • written 7 days ago by
yura.grabovska
▴ 90
0
votes
1
reply
165
views
Difference in number of reads when using duplex and simplex base calling with Dorado
base
calling
nanopore
simplex
dorado
duplex
updated 6 days ago by
GenoMax
142k • written 7 days ago by
Lélé
▴ 10
0
votes
0
replies
158
views
cat-bgen fail
bgenix
updated 6 days ago by
Ram
44k • written 6 days ago by
lambard
• 0
0
votes
0
replies
165
views
Error when running create-maf-vcf at convertGVCFToHVCFForChrom step - PHGv2
PHG
pangenome
PHG_v2
6 days ago by
Jsarria.EEAD
• 0
0
votes
0
replies
163
views
Use of ichor CNA
Dog
CNA
genome
6 days ago by
sainavyav22
• 0
0
votes
0
replies
156
views
Help interpreting KEGG module definitions for converting to NetworkX graph
definition
kegg
module
database
genomics
6 days ago by
O.rka
▴ 720
0
votes
1
reply
170
views
Add line under stat test
stats
R
updated 6 days ago by
Nyksubuz
▴ 20 • written 7 days ago by
Ghada
• 0
0
votes
4
replies
354
views
How to extract cells of different species after mapping with combined genome?
snRNA-seq
scRNA-seq
updated 5 days ago by
Tony
• 0 • written 7 days ago by
vk
▴ 10
0
votes
0
replies
140
views
How do I quantify the non-coding transcripts that I have generated after using CPAT,PLEK, and BLAST altogether?
transcript
Quantify
non-coding
5 days ago by
Varsha
• 0
0
votes
3
replies
300
views
Sam file Header problem
Sam
Header
problem
file
updated 5 days ago by
Pierre Lindenbaum
162k • written 6 days ago by
saifulislam99121
• 0
0
votes
2
replies
271
views
How to generate table_annovar from VCF input?
vcf
annovar
updated 5 days ago by
Pierre Lindenbaum
162k • written 6 days ago by
melissachua90
▴ 70
0
votes
4
replies
367
views
Merge clusters in Seurat UMAP
seurat
umap
5 days ago by
kilcdincer
▴ 10
0
votes
1
reply
465
views
solution-Transcriptome.cpp:18:Transcriptome: exiting because of *INPUT FILE* error: could not open input file /geneInfo.tab #2142
STAR
updated 5 days ago by
GenoMax
142k • written 6 days ago by
huxiangyulove
• 0
0
votes
0
replies
157
views
Understanding behaviour of SCTransform in Seurat
Seurat
scRNASeq
normalization
updated 5 days ago by
GenoMax
142k • written 5 days ago by
yura.grabovska
▴ 90
0
votes
0
replies
169
views
genomic region of transcription factor
search
HOMER
motif
5 days ago by
qudrat.nii
▴ 10
0
votes
0
replies
152
views
sci-RNA-seq
cell
single
seurat
sci-rna-seq
counts
5 days ago by
kilcdincer
▴ 10
0
votes
6
replies
392
views
ComplexHeatmap - How to change fontsize of rowAnnotation
ComplexHeatmap
5 days ago by
hannes.bongartz
• 0
0
votes
2
replies
277
views
GG Sankey plot
GO
sankey_plot
gglpot
updated 5 days ago by
LauferVA
4.2k • written 5 days ago by
ijarne
• 0
0
votes
0
replies
161
views
Cell type annotation mixed with other type cells
cell
annotation.
updated 5 days ago by
GenoMax
142k • written 5 days ago by
Long
• 0
0
votes
0
replies
153
views
How can i use ESM-1v ?
VEP
ESM-1v
5 days ago by
Meto
• 0
0
votes
7
replies
4.3k
views
B allele frequency (BAF)
snp
updated 5 days ago by
aidangcruickshank
▴ 10 • written 3.7 years ago by
rthapa
▴ 90
0
votes
4
replies
376
views
How to handle duplicated genes in TCGA data?
TCGA
GDC
mRNA
updated 4 days ago by
txema.heredia
▴ 130 • written 6 days ago by
Ngrin
• 0
0
votes
1
reply
193
views
Broad and Narrow peaks
ATAC
ChIP
seq
updated 4 days ago by
ATpoint
82k • written 4 days ago by
Jacek
▴ 20
0
votes
5
replies
328
views
calculating genomic coverage/ base overlap in R
genomics
updated 4 days ago by
1769mkc
★ 1.2k • written 7 days ago by
Xbox_27
• 0
0
votes
0
replies
150
views
Tera-scale metagenomic coassembly tool
co-assembly
Metagenomics
4 days ago by
Moinuddin
• 0
0
votes
0
replies
141
views
Buzzled by retrieving metadata on bioprojects for an organism using "rentrez" package
R
updated 4 days ago by
Pierre Lindenbaum
162k • written 4 days ago by
Mohamed Samir
▴ 30
0
votes
2
replies
306
views
Traveler with Infernal mapping failed
r2dt
updated 4 days ago by
anton.i.petrov
• 0 • written 3 months ago by
Larissa
• 0
0
votes
2
replies
326
views
Design for complex RNA-Seq experiment using Deseq2
Model.Matrix
RNA-Seq
Limma
DESeq2
edgeR
3 days ago by
Ezequiel
• 0
0
votes
0
replies
105
views
Tool for within-sample ligand-receptor analysis (bulk RNA-seq) ?
cell-cell
communication
ligand-receptor
self-regulation
3 days ago by
DGTool
▴ 20
0
votes
0
replies
115
views
handling bio replicates for chromHMM
replicates
chromHMM
3 days ago by
Hasan_Daaboul
• 0
0
votes
8
replies
545
views
Is it Possible to "Merge" data from Replicates within a VCF SNP File
snp
vcf
genomics
plink
updated 3 days ago by
Pierre Lindenbaum
162k • written 12 days ago by
ajbarrett98
• 0
0
votes
4
replies
341
views
scRNA-seq data trained model can be used for predictions on bulk RNA-seq data?
rna-seq
3 days ago by
Bibi
• 0
0
votes
0
replies
103
views
Challenges of Affymetrix probe IDs for grouping similar genes to calculate their mean
R
Mean
Affymetrix
Probes
Grouping
3 days ago by
Maryam
• 0
0
votes
1
reply
150
views
PCA Visualization error in R - arguments imply differing number of rows
SNPs
PCA
GBS
LINUX
r
updated 2 days ago by
zx8754
11k • written 3 days ago by
Ali
• 0
0
votes
0
replies
83
views
ENCORI or starBase : ceRNA data retrieval using Web API and curl
ceRNA
ENCORI
curl
microRNA
starBase
2 days ago by
V_Vibes
• 0
0
votes
4
replies
252
views
GO analysis: p-value range
GO
R
updated 2 days ago by
Matthias Zepper
4.6k • written 3 days ago by
sooni
▴ 20
0
votes
3
replies
242
views
seqtk subseq in.fastq list.txt > out.fastq not extracting full sequence from input file
subseq
seqtk
extractionproblem
updated 2 days ago by
GenoMax
142k • written 3 days ago by
KHURRAM SHAHZAD
• 0
0
votes
1
reply
141
views
GO analysis: Indicate genes corresponding to the pathway
GeneOntoloy
GO
R
updated 2 days ago by
marco.barr
▴ 130 • written 2 days ago by
sooni
▴ 20
0
votes
1
reply
106
views
How to hide label in layout file of MCscan (python version)
MCscan
updated 2 days ago by
GenoMax
142k • written 2 days ago by
jinyi
• 0
0
votes
1
reply
608
views
Illumina methylation EPIC V2 array
ewas
methylation
enrichment
missMethyl
EPIC
updated 2 days ago by
fu_entomology
▴ 40 • written 8 months ago by
juliviglino
• 0
0
votes
3
replies
202
views
GATK Structural Variants Pipeline - Steps
SV
GATK
Variant-Calling
updated 2 days ago by
Ram
44k • written 2 days ago by
Bioinformatics_begginner
▴ 20
0
votes
0
replies
102
views
transcriptome annotation
annotation
trinity
transcriptome
updated 2 days ago by
Ram
44k • written 2 days ago by
Asim Bin Arshad
• 0
0
votes
0
replies
95
views
Is it possible to keep gene_id and gene_name columns using Seurat::Read10X?
Seurat
single-cell
scRNA-seq
updated 2 days ago by
Ram
44k • written 2 days ago by
ev97
▴ 20
0
votes
5
replies
229
views
Why most genes have high padj values
RNA-seq
DEG
updated 2 days ago by
Ram
44k • written 2 days ago by
mnx0723
• 0
0
votes
2
replies
208
views
How to generate an Upset plot in R to plot the shared variants between cell free DNA samples
r
Upset
2 days ago by
sainavyav22
• 0
179 results • Page
3 of 4
Recent Votes
Error when looping over multiple columns in a data frame in R
Answer: Microbial community analysis pipelines in metagenomics
A: What are chimeric reads?
Answer: Allele count of 2 for homoplasmic MT variants in VCF
Find count of reads in BAM file that fall within BED region
Up-to-date RNA-Seq Analysis Training/Courses/Papers (Dec 2017)
Up-to-date RNA-Seq Analysis Training/Courses/Papers (Dec 2017)
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Answer: Ideal PC configurations and operating system for bioinformatics laboratory
by
emmanouil.a
▴ 120
20k are good money for some workstations ... probably you need to run more samples at the same time ... for some 1000s you could take a wor…
Comment: samtools write-index
by
LucisTheFather
• 0
``` -o alignment.sorted.bam##idx##alignment.sorted.bam.bai ``` This portion is to get .bai index, instead of .csi indexing. Although Idk th…
Comment: samtools write-index
by
LucisTheFather
• 0
Really appreciate your help Pierre! I am a little bit confused btw. do you mean this: ``` rm -vf alignment.sorted.bam.bai minimap2 -ax map-…
Comment: samtools write-index
by
LucisTheFather
• 0
Yeah, I did multiple split tests of my pipeline (just subset 400k rows of my fastq and run through my pipeline for quick tests) and none of…
Comment: Which 1000 genomes 30x files should I use for imputation?
by
David-walson
• 0
Hi there. Have you found any suggestions? I have alos been working on the 1KGP high coverage data, but i have no idea about how to use it p…
Comment: MAUVE: No gene annotations in Genbank file alignments
by
pramach1
▴ 40
The same problem persists for me too. Running progressiveMauve on command line on bunch of gbk files. Mauve itself runs fine and I do see t…
Answer: Error when looping over multiple columns in a data frame in R
by
Jeremy
▴ 910
First of all, you should use "tag.healthy" and "methods" instead of "tag.health" and "method". Also, optimal.cutpoints() wants X to be eit…
Comment: Mouse or Rat Gene Expression Data Similar to GTEx
by
GenoMax
142k
Past thread: https://www.biostars.org/p/178266/
Answer: Mouse or Rat Gene Expression Data Similar to GTEx
by
GenoMax
142k
Mouse bodymap samples : https://ncbi.nlm.nih.gov/Traces/study/?acc=PRJNA375882%20&o=acc_s%3Aa
Answer: Mouse or Rat Gene Expression Data Similar to GTEx
by
bk11
★ 2.5k
Here is the [RatGTEx][1] [1]: https://ratgtex.org
Comment: Random Access remote BAM files
by
a.penatauber
• 0
Hi Lucas, I am looking for a similar functionality as I'm working with a large volume of CRAM files on S3, and downloading them whole would…
Comment: from CRAM to fastq
by
ATpoint
82k
Yes, but I still think that with this syntax you get an uncompressed file, because samtools does not detect the gz suffix. Just take a `hea…
Comment: merge overlaps and remove non overlap genomic windows
by
reza
▴ 300
Overlap of "Ch1 200 250" and "Ch1 150 220" is "Ch1 200 220", but I want to combine windows that have overlap. Thus, my goal is to combine …
Comment: Flag multiple filtering steps on VCF files using VEP
by
Pierre Lindenbaum
162k
I don't understand that question.
Comment: merge overlaps and remove non overlap genomic windows
by
Pierre Lindenbaum
162k
> But your answer is exactly the opposite of what I want oh I see. but i don't understand the logic why: "Ch1 200 250" and "Ch1 150 220" …
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