Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Limit : today
all time
today
this week
this month
this year
49 results • Page
1 of 1
Sort: Votes
Rank
Views
Votes
Replies
20
votes
17
replies
13k
views
17 follow
Best Practice On Variant Discovery For Bacteria?
updated 6 hours ago by
Ruqaiya
• 0 • written 11.9 years ago by
lh3
33k
11
votes
13
replies
6.7k
views
7 follow
log2 fold change in RNA-seq analysis
fold
log2
change
updated 23 hours ago by
May Ling
• 0 • written 2.4 years ago by
wmsalsah
▴ 10
8
votes
9
replies
3.0k
views
7 follow
Venter Genome Vcf
vcf
updated 13 hours ago by
ericrkofman
▴ 20 • written 10.1 years ago by
win
▴ 980
4
votes
6
replies
335
views
Mouse or Rat Gene Expression Data Similar to GTEx
whole-body
expression
atlas
19 hours ago by
Shicheng Guo
★ 9.4k
4
votes
1
reply
141
views
PCA plot no distinct cluster. Only p < 0.05 indicates significant DEGs, while adj p < 0.05 does not.
RNA-seq
padj
p-value
updated 18 hours ago by
swbarnes2
14k • written 23 hours ago by
May Ling
• 0
3
votes
7
replies
3.9k
views
Difference in Bismark output methylation call files and coverage files
RRBS
Bismark
DNA-methylation
updated 23 hours ago by
Papyrus
★ 2.9k • written 3.9 years ago by
linelr
▴ 40
3
votes
3
replies
186
views
Unexpected read length from NGS
NGS
Illumina
5 hours ago by
QX
• 0
2
votes
0
replies
117
views
Job:
Permanent Position as Researcher/Computational Biologist at the National Genomics Infrastructure (Sweden)
Genomics
Sequencing
Sweden
Stockholm
SciLifeLab
23 hours ago by
Matthias Zepper
4.6k
2
votes
5
replies
240
views
Z score
Z-score
updated 23 hours ago by
Ram
44k • written 1 day ago by
Akash D
▴ 60
2
votes
6
replies
263
views
Error with BiocParallel. No barcodes files found
Barcodes
scRNA-seq
SingleCellExperiment
7 hours ago by
bio_info
▴ 10
2
votes
3
replies
159
views
How to calculate nucleotide diversity (mtDNA, PacBio_data), Suggest me some tools
NucleotideDiversity
mtDNA
PacBio
6 hours ago by
hashim.rana11
▴ 20
2
votes
4
replies
367
views
Single-cell ambient RNA correction: SoupX vs decontX contamination fraction
single-cell
ambient-RNA
updated 3 hours ago by
fracarb8
★ 1.6k • written 7 days ago by
txema.heredia
▴ 130
2
votes
2
replies
123
views
Trouble with PLINK's logistic regression analysis and covariatesTrouble with PLINK's logistic regression analysis and covariates
PLINK
GWAS
SNP
1 hour ago by
F110152169
• 0
1
vote
3
replies
233
views
Error when looping over multiple columns in a data frame in R
Statistics
R
updated 17 hours ago by
Jeremy
▴ 910 • written 1 day ago by
Mohamed Samir
▴ 30
1
vote
3
replies
96
views
Single cell analysis: Unable to subset cells in seurat object using desired nFeature_RNA cut off
subset
updated 1 hour ago by
fracarb8
★ 1.6k • written 4 hours ago by
sc_analysis
• 0
1
vote
2
replies
225
views
Construction of circos plot from WGS data
WGS
Circos
8 hours ago by
Anitha
▴ 10
0
votes
0
replies
76
views
16S analysis for specie of bacteria identification
Species-identification
16S
Kraken2
updated 17 hours ago by
Ram
44k • written 17 hours ago by
Christopher
▴ 10
0
votes
3
replies
237
views
What is a good way to do gene differentials in single cell data where one group is small 90 cells and the other group 30,000 cells or 2000 cells.
cell
RNA-Seq
single
updated 16 hours ago by
ATpoint
82k • written 18 hours ago by
shahzaibali
• 0
0
votes
2
replies
621
views
minfi::getQC - is default badsamplecutoff of 10.5 always appropriate? ~half of samples fail by this measure
getqc
ewas
minfi
updated 23 hours ago by
June
• 0 • written 10 months ago by
rkb965
• 0
0
votes
2
replies
167
views
CreateSeuratObject taking very long
seurat
10 hours ago by
eae6d2e7
• 0
0
votes
1
reply
111
views
Tools for chromosomal aneuploidy detection
genetics
aneuploidy
NGS
human
updated 9 hours ago by
cmdcolin
★ 3.8k • written 1 day ago by
adarsh_pp
▴ 40
0
votes
1
reply
93
views
Does prokka work the same on forward and reverse complementary reads?
Prokka
updated 7 hours ago by
Pierre Lindenbaum
162k • written 8 hours ago by
Maksim
• 0
0
votes
1
reply
43
views
How to calculate cell type frequency between two groups in single cell data
Seurat
single-cell
sc-RNA
cell-type
updated 24 minutes ago by
Bastien Hervé
5.3k • written 3 hours ago by
Sara
▴ 30
0
votes
0
replies
109
views
Job:
2 PhD Students in single-cell bioinformatics
PhD
Genomics
single-cell
NGS
7 hours ago by
mtabaka
• 0
0
votes
9
replies
538
views
ComplexHeatmap - How to change fontsize of rowAnnotation
r
ComplexHeatmap
updated 7 hours ago by
zx8754
11k • written 7 days ago by
hannes.bongartz
• 0
0
votes
3
replies
203
views
Splitting query fasta file for Diamond Blastp make the process faster?
DIAMOND
6 hours ago by
kmat
• 0
0
votes
0
replies
37
views
finding highly divergent orthologs using exonerate
ortholog
Exonerate
evolution
6 hours ago by
Emily
▴ 20
0
votes
1
reply
139
views
Free AI for R programming
R
updated 23 hours ago by
Ram
44k • written 1 day ago by
mohamadzare6022
▴ 10
0
votes
0
replies
6
views
Why some SNP's allele frequencies in gnomAD are so different between v2 and v4?
AF
gnomAD
SNP
10 minutes ago by
liaotsungjen
• 0
0
votes
0
replies
77
views
VEP annotation tool: "." isn't numeric in addition (+)
VEP
VCF
updated 23 hours ago by
Ram
44k • written 1 day ago by
emmanouil.a
▴ 120
0
votes
0
replies
47
views
R code for KEGG pathways output
KEGGREST
dplyr
KEGG
R
5 hours ago by
doramora
▴ 10
0
votes
1
reply
147
views
Annotating single cell data automatically
cell
annotation
single
updated 3 hours ago by
Francesco
▴ 10 • written 4 weeks ago by
Gerard
• 0
0
votes
1
reply
47
views
Overlapping clusters for different biological conditions: Seurat, UMAP
Seurat
scRNA-seq
UMAP
updated 10 minutes ago by
Bastien Hervé
5.3k • written 4 hours ago by
bio_info
▴ 10
0
votes
0
replies
28
views
Can you use a polygenic risk score for a trait (e.g., BMI) in association analyses when it doesn't associate with BMI in the sample it is used?
risk
polygenic
score
imputation
2 hours ago by
kl
▴ 10
0
votes
4
replies
92
views
Bowtie 1.3.1 alignment error as array 21720,23124 produces sam bam files
error
Bowtie
updated 2 hours ago by
GenoMax
142k • written 3 hours ago by
Deepthi
• 0
0
votes
0
replies
26
views
Harmony integration group.by.var parameter
cell
single
harmony
2 hours ago by
Picasa
▴ 640
0
votes
2
replies
67
views
How to scrape BioMart data from https://sorfs.ugent.be/ website
BioMart
1 hour ago by
QX
• 0
0
votes
0
replies
17
views
BAM to Bedgraph creating an empty file
Bisulfite-seq
bismark
1 hour ago by
irebekah.c
• 0
0
votes
0
replies
1
view
Exon Annotation failed using homer annotatePeaks.pl
annotation
annotatePeaks
splicing
alternative
homer
just now by
Alessia
• 0
0
votes
1
reply
263
views
LDhat lookup table
LDhat
updated 1 hour ago by
NÚRIA
• 0 • written 11 months ago by
Matteo
▴ 10
0
votes
1
reply
2.8k
views
bfctools merge [E::hts_open_format] Failed to open file
SNP
software error
updated 51 minutes ago by
j.f.akers
• 0 • written 3.5 years ago by
andemexoax
• 0
0
votes
0
replies
7
views
Mutation counts corrected by number of samples
mutations
WES
genomics
WGS
31 minutes ago by
garcesj
▴ 50
0
votes
1
reply
93
views
BEAST2.5 Chloroplast Divergence Time using Fossil Records
BEAST2.5
Fossil
updated 23 hours ago by
Ram
44k • written 23 hours ago by
a.bibek52
▴ 10
0
votes
2
replies
165
views
What do the transcript variant # mean in RefSeq?
refseq
updated 19 hours ago by
Ram
44k • written 22 hours ago by
curious
▴ 750
0
votes
1
reply
121
views
Trying to understand warning from MACS2 about too few paired peaks and differing results in DiffBind
MACS2
ATACseq
DiffBind
updated 19 hours ago by
jared.andrews07
★ 17k • written 23 hours ago by
atan
• 0
0
votes
0
replies
62
views
abundance_estimates_to_matrix.pl in Trinity is not generating any matrices / output
trinity
updated 18 hours ago by
GenoMax
142k • written 18 hours ago by
jway
• 0
0
votes
1
reply
98
views
minfi::getQC - badsamplecutoff 10.5
getqc
minfi
methylation
EPICv2
updated 23 hours ago by
Papyrus
★ 2.9k • written 23 hours ago by
June
• 0
0
votes
1
reply
123
views
Flag multiple filtering steps on VCF files using VEP
VEP
VCF
updated 23 hours ago by
Ram
44k • written 1 day ago by
avelarbio46
▴ 30
0
votes
4
replies
367
views
how to treat the replicates while performing WGCNA
clustering
WGCNA
replicates
17 hours ago by
mavy
▴ 10
49 results • Page
1 of 1
Recent Votes
Comment: Trouble with PLINK's logistic regression analysis and covariatesTrouble with PLI
Comment: Trouble with PLINK's logistic regression analysis and covariatesTrouble with PLI
Comment: Single cell analysis: Unable to subset cells in seurat object using desired nFea
The Biostar Herald for Monday, May 20, 2024
Answer: plink2 cannot make bed file
Answer: plink2 cannot make bed file
Answer: Conda, bioconda, anaconda, are they different?
Recent Locations •
All
Whitefish, MT,
1 minute ago
Netherlands,
1 minute ago
Spain,
3 minutes ago
United States,
3 minutes ago
Japan,
3 minutes ago
Russia,
4 minutes ago
Italy,
5 minutes ago
Recent Awards •
All
Scholar
to
chrchang523
10k
Popular Question
to
garcesj
▴ 50
Student
to
Lukas
▴ 50
Scholar
to
Michael
54k
Popular Question
to
octpus616
▴ 100
Scholar
to
ATpoint
82k
Popular Question
to
Novogene
▴ 420
Recent Replies
Comment: Overlapping clusters for different biological conditions: Seurat, UMAP
by
Bastien Hervé
5.3k
What is the need of PBMC in your analysis ? If none, removing them from the beginning will allow your clusters to be more specific to your …
Answer: How to calculate cell type frequency between two groups in single cell data
by
Bastien Hervé
5.3k
I believe `sample_id` are your replicates in either `patient` or `control`. You can do it manually by normalizing the number of cells yo…
Answer: bfctools merge [E::hts_open_format] Failed to open file
by
j.f.akers
• 0
I think the program is trying to open a .vcf.gz.csi file rather than the actual data which is the .vcf.gz file, the csi file is not data, j…
Comment: LDhat lookup table
by
NÚRIA
• 0
Hi! Did you manage to fix this? I run convert successfully on 60 unphased diploid samples (1500bp), hence I used lktable available in LDhat…
Comment: Trouble with PLINK's logistic regression analysis and covariatesTrouble with PLI
by
F110152169
• 0
It's Parkinson's disease. How do I get the right order?
Comment: How to scrape BioMart data from https://sorfs.ugent.be/ website
by
QX
• 0
yes but they did not reply
Comment: Single cell analysis: Unable to subset cells in seurat object using desired nFea
by
fracarb8
★ 1.6k
Looking at both `nCount` and `MT` the profile are cut at your thresholds, suggesting that you simply don't have cells with >2000(ish) genes…
Comment: How to scrape BioMart data from https://sorfs.ugent.be/ website
by
Pierre Lindenbaum
162k
did you ask the authors to restore their server ?
Comment: Single cell analysis: Unable to subset cells in seurat object using desired nFea
by
sc_analysis
• 0
Yes. But after applying: merged_seurat_objects_filtered <- subset(x = merged_seurat_objects, subset = nFeature_RNA > 200 & nFeature_R…
Comment: Single-cell ambient RNA correction: SoupX vs decontX contamination fraction
by
fracarb8
★ 1.6k
This [paper][1] provides a comparison of the three methods in different settings. [1]: https://genomebiology.biomedcentral.com/articles…
Comment: Bowtie 1.3.1 alignment error as array 21720,23124 produces sam bam files
by
Deepthi
• 0
pop up with sam error . Error reading _rstarts[] array: 21720, 23124
Comment: Bowtie 1.3.1 alignment error as array 21720,23124 produces sam bam files
by
ATpoint
82k
See me edit I just made for the fastq check.
Comment: Bowtie 1.3.1 alignment error as array 21720,23124 produces sam bam files
by
Deepthi
• 0
Thanks will check and update here.
Answer: Bowtie 1.3.1 alignment error as array 21720,23124 produces sam bam files
by
ATpoint
82k
bowtie -x idx file.fastq | samtools view -o out.bam You might be messing up things with these positional arguments. Use this above. Us…
Answer: Annotating single cell data automatically
by
Francesco
▴ 10
- In order to run Azimuth: ``` AzimuthClusters <- RunAzimuth(seurat_obj, reference = "pbmcref") ``` - singleR ``` surveyRefere…
Traffic: 2545 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6