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184 results • Page
1 of 4
Sort: Views
Rank
Views
Votes
Replies
76
votes
72
replies
19k
views
19 follow
Tutorial:
Clustering of DAVID gene enrichment results from gene expression studies
enrichment
david
3 days ago by
Kevin Blighe
87k
19
votes
11
replies
14k
views
10 follow
How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
utr
updated 1 day ago by
cmdcolin
★ 3.8k • written 10.8 years ago by
J.F.Jiang
▴ 920
2
votes
5
replies
10k
views
Find Corresponding Author: Doi Metadata?
updated 6 days ago by
Ram
43k • written 10.6 years ago by
Louis
▴ 150
10
votes
6
replies
9.9k
views
6 follow
CDS vs cDNA vs transcript for mapping RNA-Seq reads
Assembly
rna-seq
alignment
updated 2 days ago by
Antonio R. Franco
★ 5.1k • written 6.0 years ago by
williamsbrian5064
▴ 510
14
votes
2
replies
7.0k
views
Trouble with Pilon installation
polishing
pilon
installation
updated 3 days ago by
Lissa Cruz Saavedra
• 0 • written 7.3 years ago by
Rox
★ 1.4k
7
votes
7
replies
5.9k
views
6 follow
How to know synonymous and non-synonymous SNPs from transcriptome sequencing
RNA-Seq
SNP
updated 2 days ago by
rohitsatyam102
▴ 850 • written 6.1 years ago by
smallfish
▴ 10
8
votes
5
replies
5.3k
views
ISCN annotation for SV/CN VCF files
SV
written 7 months ago by
a.beggs
▴ 60
25
votes
11
replies
3.7k
views
6 follow
Forum:
Site Use Guidelines
meta
biostars
Guidelines
updated 4 days ago by
Ram
43k • written 14.6 years ago by
Istvan Albert
100k
2
votes
6
replies
3.7k
views
Treatment VS Control in Single Cell RNAseq analysis
Single Cell
RNAseq
DE Analysis
Treatment
Control
updated 2 days ago by
ATpoint
82k • written 3.9 years ago by
grayapply2009
▴ 280
0
votes
13
replies
3.5k
views
6 follow
Tax4Fun2 - error
tax4fun
silva
blast
updated 3 days ago by
drstalinantony28
• 0 • written 2.2 years ago by
ymj
▴ 10
0
votes
4
replies
2.7k
views
Lositan freezing when generating selection table
LOSITAN
updated 3 days ago by
evawillms183
• 0 • written 8.3 years ago by
andrepleao
• 0
2
votes
3
replies
2.5k
views
When to use .vcf or .gvcf files from GATK HaplotypeCaller?
indel
gatk
calling
snp
variant
updated 8 hours ago by
zihanss
• 0 • written 23 months ago by
Vitor1
▴ 120
1
vote
3
replies
2.5k
views
Problem to remove subset of patients with plink
plink
PED
updated 5 days ago by
chrchang523
10k • written 6.1 years ago by
Ginevra
▴ 10
2
votes
4
replies
2.4k
views
How could I generate a gi_taxid_nucl.dmp file similar to the one previously hosted by NCBI?
ncbi
metagenomics
centrifuge
gi_taxid_nucl.dmp
taxonomy
updated 3 days ago by
ZhangYuanfeng
• 0 • written 2.4 years ago by
Frazier Baker
• 0
3
votes
3
replies
2.3k
views
How to make a TSS enrichment plot in ATAC-seq analysis?
ATAC
updated 2 days ago by
sp
• 0 • written 22 months ago by
Dan
▴ 180
3
votes
6
replies
1.9k
views
7 follow
DNA methylation preprocessing
SWAN
DNA
methylation
Funnorm
updated 1 day ago by
Ram
43k • written 2.4 years ago by
SYOSY
▴ 10
2
votes
6
replies
1.1k
views
Differential Expression using Isoseq-supplemented reference transcriptome
RNA-Seq
Salmon
Isoseq
updated 1 hour ago by
Ram
43k • written 8 months ago by
Calum
▴ 10
2
votes
3
replies
954
views
Method to detect genome doubling
CNV
genome doubling
updated 5 days ago by
LauferVA
4.2k • written 4.5 years ago by
CY
▴ 750
1
vote
10
replies
938
views
Troubleshooting RNA-seq data with DNA contamination
RNA-seq
DESeq2
DNA-contamination
3 days ago by
DKA
▴ 40
8
votes
16
replies
858
views
How to convert plink files to Hapmap Format
GWAS
Plink
updated 1 day ago by
bk11
★ 2.4k • written 7 weeks ago by
Sofia
• 0
2
votes
9
replies
846
views
TCGA2STAT Error: Firehose connection
FIREHOSE
TCGA
updated 4 days ago by
LauferVA
4.2k • written 6 months ago by
Gnana
• 0
0
votes
2
replies
810
views
AGeNT LocatIt
AGeNT
LocatIt
WES
UMI
duplicates
updated 3 days ago by
barslmn
★ 2.1k • written 2.8 years ago by
jhy
▴ 10
1
vote
8
replies
805
views
Adding CB tag to bam file
samtools
bam
updated 3 days ago by
Pierre Lindenbaum
161k • written 9 days ago by
Maria
• 0
3
votes
3
replies
722
views
KissDE and batch effect
kissDE
kissplice
5 days ago by
david.b.rombaut
▴ 10
3
votes
4
replies
666
views
Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (without Blat)
mapping
5 days ago by
rahu
• 0
3
votes
6
replies
658
views
Ciriquant not configuring hisat2 indexed files
ciriquant
6 days ago by
Atul K.
• 0
5
votes
4
replies
637
views
How to compare the quality of assemblies
nextdenovo
assembly
hifiasm
pacbio
4 days ago by
kirillkirilenko
▴ 40
4
votes
11
replies
630
views
Redirection of Duplicate PMIDs
pubmed
pmid
updated 4 days ago by
LauferVA
4.2k • written 5 days ago by
dominickd
• 0
1
vote
5
replies
602
views
Cell barcode whitelists for DNBelab C Series High-throughput Single-cell RNA Series Library Preparation Set?
cell-barcode
MGI
DNBelab
single-cell
updated 4 days ago by
atowns21
• 0 • written 3 months ago by
benjamin.pyenson
• 0
0
votes
2
replies
587
views
Why cd-hit-est not work when sequence identity threshold<0.95?
genome
cluster
sequence
updated 3 days ago by
Dateline
• 0 • written 21 months ago by
JZX
• 0
4
votes
7
replies
578
views
7 follow
Heatmap and rna-seq
RNA-Seq
Heatmap
updated 1 day ago by
dsull
★ 5.9k • written 5 days ago by
qudrat.nii
▴ 10
8
votes
8
replies
572
views
PCA plot (Suggestions Needed)
DESeq2
PCAplot
updated 3 days ago by
swbarnes2
14k • written 4 days ago by
Aaliya
▴ 10
3
votes
5
replies
554
views
SLURM submission problem: Indexing the Reference Genome is not done in STAR
align
RNA-Seq
linux
STAR
6 days ago by
n_navy
• 0
7
votes
7
replies
537
views
NGS forensics: how to know if data is fabricated
fastq
STAR
NGS
Illumina
5 hours ago by
noodle
▴ 520
4
votes
8
replies
520
views
Create a new bed file with all pairwise combinations between two other bed files, based on bp distance
SNPs
BED
eqtl
bedtools
updated 1 day ago by
Alex Reynolds
35k • written 2 days ago by
J
▴ 10
0
votes
11
replies
512
views
How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq libraries?
STARSolo
scRNA-seq
STAR
snRNA-seq
MGI
2 days ago by
atowns21
• 0
0
votes
3
replies
494
views
python file for coding potential calculator
cpc2.py
updated 5 days ago by
atharvakarkare14
▴ 10 • written 9 days ago by
Ashok
• 0
7
votes
6
replies
485
views
Filtering sam or bam file with maximum matching region
crisper-edited
alignment
match
6 days ago by
analyst
▴ 30
0
votes
1
reply
460
views
GAPIT p-value significance threshold
GAPIT
p-value
GWAS
updated 2 days ago by
ginellegrenier
• 0 • written 4 months ago by
Clayton
• 0
2
votes
5
replies
457
views
Marking duplicates using UMIs
Deduplication
UMI
updated 6 days ago by
i.sudbery
19k • written 7 days ago by
Lipika
• 0
3
votes
9
replies
454
views
Is it Acceptable to Have Uniform Quality Scores in a FASTQ File?
FASTQ
updated 3 days ago by
Istvan Albert
100k • written 5 days ago by
Κοσμάς
• 0
4
votes
7
replies
450
views
To get p-values for the TPM
P-value
TPM
RSEM
updated 3 days ago by
dsull
★ 5.9k • written 4 days ago by
VITALA
• 0
0
votes
6
replies
438
views
Use of Kraken output for functional analysis
metagenomics
kraken
shotgun
updated 4 days ago by
gv
• 0 • written 26 days ago by
Srinka
▴ 20
2
votes
2
replies
432
views
Using vg gamsort with naive sorting algorithm
gamsort
vg
updated 4 days ago by
Pierre Lindenbaum
161k • written 8 months ago by
AshleeThomson
▴ 80
1
vote
4
replies
431
views
GTF file for Rhinolophus sinicus
Rhinolophus-sinicus
GTF
updated 4 days ago by
atowns21
• 0 • written 5 months ago by
ara
• 0
0
votes
3
replies
384
views
How to identify gaps in a genome?
awk
seqkit
assembly
fasta
genome
updated 4 days ago by
colindaven
6.4k • written 13 days ago by
rj.rezwan
• 0
4
votes
4
replies
378
views
Tutorial:
how to combine multiple RNAseq count files into a single dataframe in R and unix
Unix
RNAseq
R
updated 20 hours ago by
BioinfGuru
★ 1.7k • written 3 days ago by
Ming Tommy Tang
★ 3.9k
4
votes
2
replies
375
views
Missing protein (VEGF-A) in String db
VGFA
stringdb
updated 2 days ago by
shalespringer
• 0 • written 11 weeks ago by
Phenylananin
▴ 20
1
vote
6
replies
370
views
ScRNA data question
scRNA
Vlnplot
Samples
23 hours ago by
starswillfade
▴ 10
1
vote
5
replies
345
views
DYH17 Structure Prediction
BLAST
Protein-Structure-Prediction
4 days ago by
anasjamshed
▴ 120
184 results • Page
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A: Deeptools plotHeatmap - Maintain Order of Input BED file
Comment: NGS forensics: how to know if data is fabricated
Comment: calculate nucleotide diversity from whole-genome-sequence data for individual ge
Comment: calculate nucleotide diversity from whole-genome-sequence data for individual ge
Answer: DNA methylation preprocessing
Comment: NGS forensics: how to know if data is fabricated
Answer: NGS forensics: how to know if data is fabricated
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Recent Replies
Comment: gvcf joint calling
by
zihanss
• 0
Thanks for your comment, I get it now.
Answer: ChIP-seq datasets: input samples omitted?
by
ATpoint
82k
In my hands (and from what I know based on many years here) inputs are almost exclusively used during peak calling to correct for loci-spec…
Comment: How to assign cell types after integration in scRNA
by
ATpoint
82k
No difference here. After all you typically want a label per cluster/group, so either subset to the control condition to do the assignment,…
Comment: Differential Expression using Isoseq-supplemented reference transcriptome
by
gaoanwei
• 0
I found the question and ASK the GPT4 turbo
Answer: Differential Expression using Isoseq-supplemented reference transcriptome
by
gaoanwei
• 0
Your approach seems reasonable and well-informed, though it's perhaps less customary in the field, which could be why you haven't found pap…
Answer: How many reads for WGS Sequencing?
by
Mensur Dlakic
★ 27k
It takes 29 seconds to assemble this genome (20 CPUs) with the following statistics: 135 contigs, total 2821177 bp, min 200 bp, max …
Comment: NGS forensics: how to know if data is fabricated
by
noodle
▴ 520
IMO (and unfortunately) there needs to be an effort to develop these algorithms.
Comment: gvcf joint calling
by
Jeremy Leipzig
22k
the samples that are `./.` have no coverage (or not enough to call a genotype) and the `0/0` are homozygous reference
Answer: How many reads for WGS Sequencing?
by
GenoMax
141k
Did you download the complete dataset available from ENA/NCBI SRA? This is an older dataset (from 2012) with a total of 1146212 reads and 1…
Comment: When to use .vcf or .gvcf files from GATK HaplotypeCaller?
by
zihanss
• 0
Hello, I want to know that why my gVCF files have "./." besides "0/0", "1/1"? Thanks
Comment: gvcf joint calling
by
zihanss
• 0
![enter image description here][1] [1]: /media/images/15eedc1a-b2c6-4966-be39-b5173dab And I confused with the file that has "./." and…
Comment: gvcf joint calling
by
zihanss
• 0
![enter image description here][1] [1]: /media/images/16fd502c-4e01-4f56-8562-0e0d4aac Okay, this is the merged gVCF file.
Comment: Help understanding how KEGG Ortholog `K00004 ` has 3 ECs associated with it (EC:
by
Mensur Dlakic
★ 27k
Enzymes under the umbrella of `1.1.1.-` work `with NAD(+) or NADP(+) as acceptor`. That only tells you about their cofactors, but not about…
Comment: NGS forensics: how to know if data is fabricated
by
dsull
★ 5.9k
I don't think people have undertaken the effort to create an anomaly detector for RNAseq -- people's efforts are dedicated towards developi…
Comment: NGS forensics: how to know if data is fabricated
by
dsull
★ 5.9k
I'd say post on pubpeer -- it's the best forum for this sort of discussion. As for what additional analysis I recommend: I'd say look at s…
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