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12,664 results • Page
1 of 254
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0
votes
0
replies
4
views
Cell type annotation mixed with other type cells
cell
annotation.
2 minutes ago by
Long
• 0
0
votes
2
replies
81
views
Trimming tool
Trimmer
tool
updated 1 hour ago by
ntsopoul
▴ 60 • written 3 hours ago by
GeneC
• 0
0
votes
0
replies
30
views
sci-RNA-seq
cell
single
seurat
sci-rna-seq
counts
2 hours ago by
kilcdincer
▴ 10
0
votes
0
replies
37
views
Design for complex RNA-Seq experiment using Deseq2
Model.Matrix
RNA-Seq
Limma
DESeq2
edgeR
3 hours ago by
Ezequiel
• 0
0
votes
1
reply
46
views
GG Sankey plot
GO
sankey_plot
gglpot
updated 29 minutes ago by
bk11
★ 2.5k • written 4 hours ago by
ijarne
• 0
797
votes
167
replies
143k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 3 months ago by
Biostar
2.8k • written 7.4 years ago by
Istvan Albert
100k
0
votes
0
replies
59
views
genomic region of transcription factor
search
HOMER
motif
4 hours ago by
qudrat.nii
▴ 10
1
vote
5
replies
251
views
Reconstruction of locus, order contigs
contigs
cluster
alignment
locus
updated 4 hours ago by
dthorbur
★ 2.0k • written 22 hours ago by
BATMAN
• 0
0
votes
5
replies
165
views
ComplexHeatmap - How to change fontsize of rowAnnotation
ComplexHeatmap
updated 3 hours ago by
bk11
★ 2.5k • written 7 hours ago by
hannes.bongartz
• 0
0
votes
0
replies
53
views
Understanding behaviour of SCTransform in Seurat
Seurat
scRNASeq
normalization
updated 8 hours ago by
GenoMax
142k • written 9 hours ago by
yura.grabovska
▴ 90
4
votes
7
replies
434
views
In one PCA plot, can I calculate the percentage of different factors that contribute to the PCA?
PCA
R
VARIANCE
updated 9 hours ago by
marco.barr
▴ 130 • written 2 days ago by
diqixiaoyaoer
▴ 20
0
votes
0
replies
59
views
constructing pangenome through psvcp
psvcp
pangenome
11 hours ago by
analyst
▴ 50
1
vote
9
replies
258
views
zero TPM for many gene including housekeeping when using kallisto
RNAseq
5 hours ago by
ashkan
▴ 160
0
votes
1
reply
94
views
What marks a De-Novo Genome assembly as FAILED?
nanopore
denovo
illumina
assembly
genome
updated 10 hours ago by
dthorbur
★ 2.0k • written 12 hours ago by
Umer
▴ 50
0
votes
2
replies
156
views
How to handle duplicated genes in TCGA data?
TCGA
GDC
mRNA
updated 5 hours ago by
Zhenyu Zhang
★ 1.2k • written 21 hours ago by
Ngrin
• 0
4
votes
9
replies
396
views
Faster Needleman-Wunsch rapid global alignment of two sequences?
Needleman-Wunsch
alignment
8 hours ago by
Gabriel R.
★ 2.9k
0
votes
2
replies
154
views
How to generate table_annovar from VCF input?
vcf
annovar
updated 13 hours ago by
Pierre Lindenbaum
161k • written 20 hours ago by
melissachua90
▴ 70
0
votes
0
replies
61
views
How do I quantify the non-coding transcripts that I have generated after using CPAT,PLEK, and BLAST altogether?
transcript
Quantify
non-coding
15 hours ago by
Varsha
• 0
0
votes
1
reply
179
views
solution-Transcriptome.cpp:18:Transcriptome: exiting because of *INPUT FILE* error: could not open input file /geneInfo.tab #2142
STAR
updated 11 hours ago by
GenoMax
142k • written 18 hours ago by
huxiangyulove
• 0
0
votes
0
replies
79
views
Help interpreting KEGG module definitions for converting to NetworkX graph
definition
kegg
module
database
genomics
21 hours ago by
O.rka
▴ 720
4
votes
5
replies
4.3k
views
Tool:
GRIDSS: the Genomic Rearrangement IDentification Software Suite
Assembly
Structural-Variation
Variant-Calling
updated 22 hours ago by
dario.garvan
▴ 520 • written 7.2 years ago by
d-cameron
★ 2.9k
0
votes
4
replies
255
views
Merge clusters in Seurat UMAP
seurat
umap
13 hours ago by
kilcdincer
▴ 10
0
votes
0
replies
82
views
Use of ichor CNA
Dog
CNA
genome
1 day ago by
sainavyav22
• 0
2
votes
2
replies
196
views
Single-cell ambient RNA correction: SoupX vs decontX contamination fraction
single-cell
ambient-RNA
updated 1 day ago by
Ram
43k • written 1 day ago by
txema.heredia
▴ 130
1
vote
0
replies
97
views
News:
Course on Manual Genome Curation
Genome-Assembly
Pretext-View
Manual-Genome-Curation
updated 22 hours ago by
Ram
43k • written 1 day ago by
carlopecoraro2
★ 2.5k
6
votes
4
replies
265
views
Details on salmon index
Salmon
updated 8 hours ago by
Rob
6.6k • written 1 day ago by
Lorenzo
• 0
0
votes
0
replies
88
views
Error when running create-maf-vcf at convertGVCFToHVCFForChrom step - PHGv2
PHG
pangenome
PHG_v2
1 day ago by
Jsarria.EEAD
• 0
0
votes
1
reply
140
views
How can I calculate the OS of each patient?
overall-survival
updated 1 day ago by
ATpoint
82k • written 1 day ago by
Pedro
• 0
1
vote
1
reply
148
views
mitochondrial genome assembly pipeline
mammals
mitochondrial-genome
updated 1 day ago by
Ram
43k • written 1 day ago by
m90
▴ 30
0
votes
0
replies
87
views
cat-bgen fail
bgenix
updated 1 day ago by
Ram
43k • written 1 day ago by
lambard
• 0
3
votes
4
replies
268
views
Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer version?
RNA-seq
patch
freeze
genome
updated 6 hours ago by
Jeremy Leipzig
22k • written 1 day ago by
Ali
• 0
3
votes
3
replies
187
views
getting exon coordinates
genomics
updated 1 day ago by
Ram
43k • written 1 day ago by
Xbox_27
• 0
0
votes
4
replies
262
views
How to extract cells of different species after mapping with combined genome?
snRNA-seq
scRNA-seq
updated 15 hours ago by
Tony
• 0 • written 1 day ago by
vk
▴ 10
1
vote
5
replies
242
views
Can I perform a correlation test with 3 biological replicates per condition?
RNA-Seq
deseq2
updated 22 hours ago by
Ram
43k • written 1 day ago by
manuelmourato25
• 0
3
votes
4
replies
352
views
finding evidence(s) of a peptide translated from an "Upstream Open Reading Frame (uORF)"
UTR
masspec
uORF
peptide
updated 1 day ago by
GenoMax
142k • written 1 day ago by
Pierre Lindenbaum
161k
2
votes
11
replies
646
views
DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts in whole blood: is it normal to always get NS?
rstudio
DEG
limma
14 hours ago by
egascon
• 0
1
vote
1
reply
147
views
API kegg - IndexError: list index out of range
kegg
API
updated 18 hours ago by
Nyksubuz
▴ 20 • written 1 day ago by
mirwa.zidi93
• 0
0
votes
0
replies
92
views
Blog:
The Current State and Future Prospects of Long-Read Sequencing
LRS
1 day ago by
Novogene
▴ 420
0
votes
0
replies
96
views
qPCR gene expression data analysis
qPCR
geneexpression
anova
1 day ago by
ebertomeup
• 0
0
votes
3
replies
229
views
Sam file Header problem
Sam
Header
problem
file
updated 14 hours ago by
Pierre Lindenbaum
161k • written 1 day ago by
saifulislam99121
• 0
1
vote
4
replies
228
views
Tool:
Converting Degree Minutes (DM) to Decimal Degrees (DD) using python script
Minutes
Decimal
offtopic
Degrees
python
updated 22 hours ago by
Ram
43k • written 2 days ago by
kuttibiotech2009
▴ 30
0
votes
1
reply
557
views
scRNAseq quality control weird double curve in nFeature vs nCount plot
scRNA-seq
QC
single
cell
BDRhapsody
analysis
updated 1 day ago by
valdirbarth
▴ 20 • written 12 months ago by
Salomé
• 0
0
votes
0
replies
110
views
select set of intervals that cover a genomic region
GRanges
bed
1 day ago by
ntsopoul
▴ 60
1
vote
1
reply
158
views
Tool:
Collect dispersed executable files and run them by using bingo.
bingo
updated 22 hours ago by
Ram
43k • written 1 day ago by
dwpeng
▴ 10
0
votes
1
reply
137
views
BiomartException: Query ERROR for existing dataset in BioMart
biomart
ensembl
wheat
python
pybiomart
updated 16 hours ago by
Nyksubuz
▴ 20 • written 1 day ago by
Luqman
• 0
2
votes
3
replies
218
views
ggrepel function: geom_text_repel()
ggplot2
ggrepel
R
updated 22 hours ago by
Ram
43k • written 1 day ago by
sooni
▴ 20
0
votes
3
replies
197
views
how to treat the replicates while performing WGCNA
clustering
WGCNA
replicates
updated 5 hours ago by
andres.firrincieli
3.6k • written 1 day ago by
mavy
▴ 10
1
vote
1
reply
228
views
Calculate allelic frequency from VEP output vcf file
VEP
variant
2 days ago by
ramiro.barrantes
• 0
2
votes
6
replies
1.9k
views
How can you *consistently* download BioProject IDs from NCBI's BioSample database using Entrez Direct?
XML
ncbi
entrez-direct
updated 2 days ago by
Ram
43k • written 3.9 years ago by
millere
• 0
0
votes
1
reply
164
views
What's next after GATK variant calling pipeline?
GATK
pipeline
figures
DNA-seq
updated 1 day ago by
Michael
54k • written 2 days ago by
mgranada3
▴ 30
12,664 results • Page
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A: Peaks And Nearby Genes
Answer: How to download dbSNP153 vcf files in hg19/GRCH37 version
Produce PCA bi-plot for 1000 Genomes Phase III - Version 2
Produce PCA bi-plot for 1000 Genomes Phase III - Version 2
Answer: TFs and Gene databases
A: Is there a software that can compare phylogenetic trees to each othe (face to fa
Comment: Differentially Expressed Genes between two conditions (scRNA, single GEO dataset
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• 0
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▴ 170
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Recent Replies
Comment: GG Sankey plot
by
bk11
★ 2.5k
You need to change your data table into a 'long' format. You can do something like this- GOs_childs_recursive <- GOs_childs_recursive …
Answer: Trimming tool
by
ntsopoul
▴ 60
I use Trim Galore which automatically recognizes adaptors. https://github.com/FelixKrueger/TrimGalore Here is the script I am using for…
Answer: Trimming tool
by
GenoMax
142k
There are plenty. `bbduk.sh` from BBMap suite (https://jgi.doe.gov/data-and-tools/software-tools/bbtools/bb-tools-user-guide/bbduk-guide/ )…
Comment: ComplexHeatmap - How to change fontsize of rowAnnotation
by
bk11
★ 2.5k
Please check out [in this link.][1] [1]: https://github.com/jokergoo/ComplexHeatmap/issues/349
Comment: Reconstruction of locus, order contigs
by
dthorbur
★ 2.0k
[Here](https://www.biostars.org/p/9544689/) is a forum post that may answer your question.
Comment: zero TPM for many gene including housekeeping when using kallisto
by
ashkan
▴ 160
@atpoint I checked all transcripts of GAPDH. they all have 0 TPM.
Comment: how to treat the replicates while performing WGCNA
by
andres.firrincieli
3.6k
> 1) I do not want to merge the MEs rather I asked about replicates, as > some replicates of same sample are showing different behavior for…
Comment: How to systematically check if a bam file is truncated
by
alanh
▴ 170
For more details about what `samtools quickcheck` actually checks for, add a few `-v`'s to the command line. $ samtools quickcheck -…
Comment: How to handle duplicated genes in TCGA data?
by
Zhenyu Zhang
★ 1.2k
Second what is said above, ensembl_id is the unique identifier. As a good bioinformatics habit, always use unique identifier for your anal…
Comment: ComplexHeatmap - How to change fontsize of rowAnnotation
by
hannes.bongartz
• 0
It doesn't work. The annotation labels are still in a default fontsize. I cant find a way to set the fontsize.
Comment: ComplexHeatmap - How to change fontsize of rowAnnotation
by
Ram
43k
Yes. The solution to most things with ComplexHeatmap is to play around with parameters.
Comment: Reconstruction of locus, order contigs
by
BATMAN
• 0
I do not want to re-annotate the genome. I ask if there is a way to extract the region aligned with the mauve, in .gbk format.
Comment: ComplexHeatmap - How to change fontsize of rowAnnotation
by
hannes.bongartz
• 0
Thank you. What exactly do you mean? Changing `ha = rowAnnotation(text = anno_mark(at = mark_at, labels = gene_names_interesting_in_cluster…
Comment: ComplexHeatmap - How to change fontsize of rowAnnotation
by
Ram
43k
Try tweaking the `gp` of the rowAnnotation instead of tweaking the `annotation_name_gp` - that might do the trick.
Comment: Help! Reconstruction of locus, order contigs
by
dthorbur
★ 2.0k
I don't understand your comment. What do you mean? > my idea is not to have to write it down If you have an alignment already, why can't…
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