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178 results • Page
2 of 4
Sort: Views
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Views
Votes
Replies
0
votes
3
replies
354
views
Tool:
Introducing NovaDemux, a Sequence Demultiplexer that Increases Yield and Salvages Failed Runs
NovaDemux
NovaSeq
Illumina
Demultiplexing
Barcode
2 days ago by
Brian Bushnell
20k
0
votes
0
replies
350
views
ERROR with Maker annotation: The assumed RepeatMasker installation directory /opt/home/sony/miniconda3/envs/MAKER2/share/RepeatMasker does not ap…
RepeatMasker.
MAKER.
annotation.
2 days ago by
Sony
▴ 10
3
votes
4
replies
350
views
Getting differential ChIP-seq peaks between conditions after calling consensus peaks
MAnorm
ChIP-seq
consensus
peaks
1 day ago by
Alexandra
• 0
0
votes
4
replies
343
views
BiomartException: Query ERROR for existing dataset in BioMart
biomart
ensembl
wheat
python
pybiomart
5 days ago by
Luqman
• 0
4
votes
6
replies
336
views
Error with BiocParallel. No barcodes files found
Barcodes
scRNA-seq
SingleCellExperiment
3 days ago by
bio_info
▴ 20
1
vote
3
replies
324
views
Filtering VCF files based on VAF giving incorrect results
GATK
picard
VCF
bcftools
updated 6 days ago by
Ram
44k • written 12 days ago by
Arton
▴ 10
2
votes
6
replies
324
views
How to get the ratio of allele counts from GATK derived VCF file?
Freebayes
GATK
SNP
VCF
4 days ago by
anikcropscience
▴ 230
1
vote
3
replies
322
views
Error when looping over multiple columns in a data frame in R
Statistics
R
updated 3 days ago by
Jeremy
▴ 910 • written 4 days ago by
Mohamed Samir
▴ 30
1
vote
4
replies
321
views
How to find SRA sequences of some fungal whole genome sequences if only Biosample ID is available?
SRA
whole-genome-sequencing
NCBI
updated 6 days ago by
Ram
44k • written 7 days ago by
Harshita
• 0
2
votes
4
replies
318
views
Functional enrichment analysis for unique gene IDs
RNA-SEQ
32 minutes ago by
Pegasus
▴ 100
1
vote
4
replies
317
views
Single cell analysis: Unable to subset cells in seurat object using desired nFeature_RNA cut off
single-cell
3 days ago by
sc_analysis
• 0
1
vote
2
replies
315
views
Error when i use EVidenceModeler to do the genome annotation
EVidenceModeler
annotation
genome
2 days ago by
peanut
• 0
2
votes
3
replies
309
views
How do we do quantification using stringtie merge option for all the merged samples generated.
stringtie
quantification
9 hours ago by
Varsha
• 0
2
votes
5
replies
306
views
Z score
Z-score
updated 4 days ago by
Ram
44k • written 4 days ago by
Akash D
▴ 60
0
votes
3
replies
306
views
Kraken2 Custom Database non-deterministic results
Metagenomics
kraken2
updated 5 days ago by
colindaven
6.4k • written 7 days ago by
Bjorn
• 0
0
votes
6
replies
289
views
from CRAM to fastq
FASTQ
WGS
CRAM
updated 4 days ago by
ATpoint
82k • written 5 days ago by
machaalani29
• 0
1
vote
4
replies
287
views
Genotyping sites with N in reference genome
GATK
UnifiedGenotyper
5 days ago by
shpak.max
▴ 50
1
vote
3
replies
282
views
How to calculate cell type frequency between two groups in single cell data
Seurat
single-cell
sc-RNA
cell-type
updated 2 days ago by
Bastien Hervé
5.3k • written 3 days ago by
Sara
▴ 30
1
vote
2
replies
279
views
Trouble with PLINK's logistic regression analysis and covariatesTrouble with PLINK's logistic regression analysis and covariates
PLINK
GWAS
SNP
3 days ago by
F110152169
• 0
2
votes
4
replies
279
views
Allele count of 2 for homoplasmic MT variants in VCF
Variant-Calling
GATK
VCF
4 days ago by
Arton
▴ 10
0
votes
1
reply
270
views
GWAS Phenotypes
GWAS
updated 4 days ago by
韩雨
• 0 • written 5 weeks ago by
solomoncharles77
▴ 90
0
votes
3
replies
266
views
Error in using BioSampleParser tool: subscript out of bounds
R
updated 6 days ago by
marco.barr
▴ 130 • written 7 days ago by
Mohamed Samir
▴ 30
2
votes
3
replies
261
views
How to calculate nucleotide diversity (mtDNA, PacBio_data), Suggest me some tools
NucleotideDiversity
mtDNA
PacBio
3 days ago by
hashim.rana11
▴ 20
1
vote
2
replies
258
views
Failed to download data from EBI with ascp
EBI
aspera
updated 12 hours ago by
孝中
• 0 • written 6 weeks ago by
biock
▴ 60
0
votes
3
replies
254
views
z-score of gene set
z-score
scRNA
gene-set
5 days ago by
Hien
• 0
0
votes
1
reply
253
views
Annotating single cell data automatically
single-cell
updated 3 days ago by
Ram
44k • written 5 weeks ago by
Gerard
• 0
0
votes
5
replies
252
views
Bowtie 1.3.1 alignment error as array 21720,23124 produces sam bam files
Bowtie
3 days ago by
Deepthi
• 0
0
votes
4
replies
251
views
Where to find old version of GATK best practice
gatk
6 days ago by
Zhenyu Zhang
★ 1.2k
2
votes
4
replies
251
views
Filter Genome for Specific Sites
bedtools
updated 6 days ago by
Ram
44k • written 7 days ago by
Anita
• 0
0
votes
2
replies
248
views
What do the transcript variant # mean in RefSeq?
refseq
updated 4 days ago by
Ram
44k • written 4 days ago by
curious
▴ 750
1
vote
2
replies
247
views
News:
ICGEB - SLIBTEC NGS Workshop: Won Best Oral Presentation Award
BestTalk
PlatformPresentation
offtopic
updated 6 days ago by
colindaven
6.4k • written 7 days ago by
Dr.
• 0
0
votes
4
replies
245
views
Fewer sites in vcf than in reference genome despite Emit_All_Sites argument
GATK
UnifiedGenotyper
5 days ago by
shpak.max
▴ 50
1
vote
2
replies
242
views
Construction of circos plot from WGS data
WGS
Circos
3 days ago by
Anitha
▴ 10
1
vote
3
replies
237
views
Trimmomatic running but files containing purged reads are empty
fastqc
fastq
trimmomatic
updated 5 days ago by
GenoMax
142k • written 5 days ago by
Wilber0x
▴ 50
4
votes
1
reply
235
views
PCA plot no distinct cluster. Only p < 0.05 indicates significant DEGs, while adj p < 0.05 does not.
RNA-seq
padj
p-value
updated 3 days ago by
swbarnes2
14k • written 4 days ago by
May Ling
• 0
0
votes
3
replies
233
views
How to add Ensembl ids after Pseudobulk analysis by DESeq2
Seurat
Pseudobulk
single-cell
DESeq2
scRNA
updated 6 hours ago by
ATpoint
82k • written 1 day ago by
Sara
▴ 30
3
votes
3
replies
230
views
Unexpected read length from NGS
NGS
Illumina
3 days ago by
QX
• 0
0
votes
4
replies
226
views
merge overlaps and remove non overlap genomic windows
BED
intersection
overlap
4 days ago by
reza
▴ 300
4
votes
2
replies
219
views
Number of non-ATCG nucleotides replaced by Salmon
rna-seq
stringtie
indexing
salmon
updated 2 days ago by
Rob
6.6k • written 2 days ago by
Tonya S.
▴ 10
1
vote
2
replies
218
views
Elbow plot question (scRNA seq data analysis - scanpy tutorial)
scanpy
6 days ago by
ojaswinipandey
• 0
3
votes
2
replies
218
views
converting transcript IDs (Ensembl) to gene symbols in R
RNA-seq
updated 5 days ago by
GenoMax
142k • written 6 days ago by
ashkan
▴ 160
0
votes
3
replies
217
views
Splitting query fasta file for Diamond Blastp make the process faster?
DIAMOND
3 days ago by
kmat
• 0
2
votes
0
replies
217
views
Job:
Permanent Position as Researcher/Computational Biologist at the National Genomics Infrastructure (Sweden)
Genomics
Sequencing
Sweden
Stockholm
SciLifeLab
4 days ago by
Matthias Zepper
4.6k
0
votes
2
replies
216
views
Comparing Analysis Strategies for scRNA-seq Data: Separate vs. Merged Analysis of Spleen Samples from Different Conditions
Seurat
scRNA-Seq
Single-Cell
6 days ago by
Shukai
• 0
5
votes
3
replies
214
views
FastQC interpretation - 16S sequencing
interpretation
amplicon
Fastqc
16S
updated 5 days ago by
GenoMax
142k • written 5 days ago by
Christopher
▴ 10
0
votes
2
replies
213
views
perl Error - needLargeMem Bigwig
Bigwig
perl
mitosalt
8 hours ago by
marco.barr
▴ 130
1
vote
2
replies
211
views
Setting a threshold on gene expression for subsetting (scRNA-seq)
scRNA-seq
updated 1 day ago by
LauferVA
4.2k • written 2 days ago by
carolofharvest
▴ 40
1
vote
3
replies
210
views
Question about samtools view flags (paired reads vs. properly paired reads)
samtools
updated 5 days ago by
Pierre Lindenbaum
162k • written 6 days ago by
mrk
• 0
0
votes
2
replies
209
views
Tools for chromosomal aneuploidy detection
genetics
aneuploidy
NGS
human
updated 10 hours ago by
a.alnawfal.1992
▴ 260 • written 4 days ago by
adarsh_pp
▴ 40
1
vote
3
replies
208
views
downloading chemical database from ChEMBL
ChEMBL
PyRMD
Virtual-Screening
updated 5 days ago by
GenoMax
142k • written 5 days ago by
s
• 0
178 results • Page
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Comment: Functional enrichment analysis for unique gene IDs
Answer: How do i calculate the mean of triplicates in a data.frame based on pattern?
How do i calculate the mean of triplicates in a data.frame based on pattern?
C: snpEff assigned all variants as modifier intergenic
A: Problem in indexing toplevel genome with HISAT2
C: The Biostar Handbook. A bioinformatics e-book for beginners.
Answer: Error with BiocParallel. No barcodes files found
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Recent Replies
Comment: Functional enrichment analysis for unique gene IDs
by
Pegasus
▴ 100
Since the locus-tags are unclassified, I used the CDS instead of the locus-tags to retrive their ensembl IDs using ensembl bacteria website…
Comment: samtools mpileup error - 1 samples in 1 input files
by
Damla
• 0
Could you find a solution to your problem?
Comment: txt file to bigwig
by
sogand
• 0
Hi all, I am new to these kind of analysis and appreciate any guidance on my issue here: I have analyzed peakcalling .txt files that are r…
Answer: Help me with Heatmap
by
MolGeek
▴ 50
Hello! So, what is the correct design ? You will have to ask yourself, what do you want to compare? WT vs mut? If so it will be strain. 2…
Answer: Help me with simple data for RNA seq
by
swbarnes2
14k
1) you have to say what question you are actually asking. There are few different designs that would address different questions. 2) work…
Comment: identify the coordinate for coding and non_coding region.
by
Ghada
• 0
mmmm I am not sure. This is how I generate my consnsus sequence # Get consensus fastq file samtools mpileup -uf KT992094.1.fasta…
Comment: identify the coordinate for coding and non_coding region.
by
GenoMax
142k
Why do you have those N's at the beginning of the sequence? If the remainder of the sequence matches 100% then the initial N's may be wrong…
Answer: Salmon vs Kallisto vs RSEM
by
Rob
6.6k
It's nice that you've gone ahead and done some analysis of these results and their differences on your data yourself. These more general qu…
Comment: Python script to query GeneCards to get EntrezID, symbol from Ensembl geneID
by
Mike Smith
★ 2.0k
In this location there is an "entrez" file which maps ensembl IDs to Entrez. It's probably as definitive as you're going to find: https://…
Comment: Python script to query GeneCards to get EntrezID, symbol from Ensembl geneID
by
Anya
• 0
Tried it just recently with almost the same results. 456 out of 496 IDs for protein-coding genes returned as "NA" even though I can see NCB…
Answer: Download All The Bacterial Genomes From Ncbi
by
2284046470
• 0
wget https://ftp.ncbi.nlm.nih.gov/genomes/refseq/bacteria/assembly_summary.txt awk -F '\t' '{if($12=="Complete Genome" && $11=…
Answer: How do i calculate the mean of triplicates in a data.frame based on pattern?
by
dariober
14k
I'm a happy user of [data.table](https://rdatatable.gitlab.io/data.table/): ``` library(data.table) dat <- structure(list(sgrna = c("Cont…
Comment: How do i calculate the mean of triplicates in a data.frame based on pattern?
by
Assa Yeroslaviz
★ 1.9k
I know it can be done with something like that: ``` cbind(p767.AM = (rowMeans(p767[,2:4])), p767.MM = (rowMeans(p767[,5:7]…
Comment: How to add Ensembl ids after Pseudobulk analysis by DESeq2
by
ATpoint
82k
Why is your `GeneName` column in mapped a mix of Ensembl IDs and gene names? What Jared wants to say is that during the preprocessing you s…
Comment: Python script to query GeneCards to get EntrezID, symbol from Ensembl geneID
by
GenoMax
142k
> only give me partial results It is possible that not every Ensembl ID is going to convert to Entrez ID. Have you tried: https://www.b…
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