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182 results • Page
2 of 4
Sort: Views
Rank
Views
Votes
Replies
0
votes
4
replies
304
views
Correlation Analysis
statistics
methylation
NGS
expression
6 days ago by
Researcher
▴ 30
0
votes
2
replies
296
views
Extracting mutation status from MAF files - TCGA
TCGA
maf
mutation
updated 4 days ago by
Zhenyu Zhang
★ 1.2k • written 14 days ago by
yahn
• 0
0
votes
2
replies
294
views
How are score_weights calculated in this code?
single-cell
6 days ago by
carolofharvest
▴ 40
2
votes
4
replies
294
views
Benchmarking RNASeq Variant Calling Pipeline (Short Reads)
rna-seq
vcf
variant-calling
2 days ago by
Esraa
• 0
1
vote
4
replies
284
views
Add stats to boxplot in R
stats
R
2 days ago by
Ghada
• 0
1
vote
4
replies
282
views
Filtering VCF files based on VAF giving incorrect results
bcftools
GATK
Bioinformatics
picard
VCF
2 days ago by
Arton
▴ 10
3
votes
2
replies
281
views
imputation through beagle
panel
beagle
reference
imputation
3 days ago by
analyst
▴ 50
0
votes
5
replies
281
views
bbmerge (bbmap) ~ error with insert size file output
bbtools
bbmerge
bbmap
updated 1 day ago by
GenoMax
142k • written 2 days ago by
chrisk
• 0
2
votes
3
replies
280
views
error in fun(x((i)), ...) : only defined on a data frame with all numeric alike variables
studio
Cibersort
R
4 days ago by
Azra
▴ 10
1
vote
3
replies
278
views
Using ggplotly in R
ggplot
ggplotly
updated 2 days ago by
jared.andrews07
★ 16k • written 3 days ago by
jen
▴ 10
2
votes
3
replies
276
views
Finding variants within a subset of a BAM file
variant
calling
3 days ago by
ramiro.barrantes
• 0
1
vote
3
replies
270
views
Is there any way to modify this pie chart ?
pie
ggplot
R
chart
updated 1 day ago by
GenoMax
142k • written 3 days ago by
ohtang7
▴ 40
2
votes
4
replies
269
views
Can 5' and 3' scRNAseq be processed with the same pipeline?
singlecell
seurat
10XGenomics
scRNAseq
updated 2 days ago by
yura.grabovska
▴ 90 • written 2 days ago by
ev97
▴ 20
1
vote
2
replies
268
views
How to interpret infinite odds ratio?
statistics
3 days ago by
Lukas
• 0
3
votes
3
replies
268
views
Gene Specific coverage from WGS data
WGS
SARS-CoV2
updated 4 days ago by
Ram
43k • written 17 days ago by
Adyasha
• 0
2
votes
5
replies
268
views
What does the 'E%' represent in BUSCO results?
BUSCO
updated 3 days ago by
Philipp Bayer
8.5k • written 3 days ago by
林明德
• 0
3
votes
4
replies
268
views
Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer version?
RNA-seq
patch
freeze
genome
updated 6 hours ago by
Jeremy Leipzig
22k • written 1 day ago by
Ali
• 0
6
votes
4
replies
266
views
Details on salmon index
Salmon
updated 8 hours ago by
Rob
6.6k • written 1 day ago by
Lorenzo
• 0
2
votes
1
reply
263
views
Practical Haplotype Graph v2 not finding correct paths
Pangenome
PHG
graph
updated 5 days ago by
pjb39
▴ 210 • written 7 days ago by
beantkapoor16
▴ 10
0
votes
4
replies
263
views
How to extract cells of different species after mapping with combined genome?
snRNA-seq
scRNA-seq
updated 16 hours ago by
Tony
• 0 • written 1 day ago by
vk
▴ 10
1
vote
9
replies
260
views
zero TPM for many gene including housekeeping when using kallisto
RNAseq
5 hours ago by
ashkan
▴ 160
1
vote
5
replies
259
views
Annotating file using bcftools
annotation
plink
bcftools
1 day ago by
kl
▴ 10
0
votes
3
replies
259
views
RNAseq RNA content
mRNA
Linux
rRNA
RNA-seq
RNA
updated 3 days ago by
noodle
▴ 580 • written 3 days ago by
doramora
▴ 10
0
votes
4
replies
258
views
Galaxy StringTie error
stringtie
galaxy
3 days ago by
trkfs
• 0
0
votes
4
replies
256
views
Merge clusters in Seurat UMAP
seurat
umap
13 hours ago by
kilcdincer
▴ 10
1
vote
5
replies
253
views
Reconstruction of locus, order contigs
contigs
cluster
alignment
locus
updated 4 hours ago by
dthorbur
★ 2.0k • written 22 hours ago by
BATMAN
• 0
0
votes
1
reply
246
views
Two-Sample Mendelian Randomization: Association between Instrumental Variable and Outcome
Mendelian-Randomization
updated 4 days ago by
Ram
43k • written 8 days ago by
Nikki
• 0
0
votes
4
replies
244
views
calculating genomic coverage/ base overlap in R
genomics
updated 1 day ago by
Ram
43k • written 2 days ago by
Xbox_27
• 0
1
vote
5
replies
242
views
Can I perform a correlation test with 3 biological replicates per condition?
RNA-Seq
deseq2
updated 23 hours ago by
Ram
43k • written 1 day ago by
manuelmourato25
• 0
0
votes
1
reply
242
views
Nomalization - TCGA, RNA-seq and Microarray
TCGA
RNA-seq
Nomalization
Microarray
updated 4 days ago by
Zhenyu Zhang
★ 1.2k • written 7 days ago by
jain72744
▴ 10
0
votes
4
replies
239
views
Sequencing Depth (Read Depth) Calculations
depth
1 day ago by
LucisTheFather
• 0
2
votes
1
reply
234
views
Failed to open VCF file
GATK
sentieon
BWA-MEM
updated 6 days ago by
Pierre Lindenbaum
161k • written 6 days ago by
melissachua90
▴ 70
0
votes
1
reply
233
views
input file in rmats
rmats
updated 5 days ago by
Mathew
▴ 140 • written 7 days ago by
Lambodarswain316
• 0
0
votes
2
replies
231
views
java.nio.bufferunderflowexception haplotypecaller error for bqsr reads
deducplicated
java.nio.bufferunderflowexception
haplotypecaller
bqsr
4 days ago by
analyst
▴ 50
0
votes
2
replies
230
views
Obtaining Infinium Methylation450K manifest for hg38
TCGA
hg38
methylation
Illumina
updated 4 days ago by
Zhenyu Zhang
★ 1.2k • written 9 days ago by
elisheva
▴ 120
0
votes
3
replies
230
views
Sam file Header problem
Sam
Header
problem
file
updated 14 hours ago by
Pierre Lindenbaum
161k • written 1 day ago by
saifulislam99121
• 0
1
vote
1
reply
229
views
Calculate allelic frequency from VEP output vcf file
VEP
variant
2 days ago by
ramiro.barrantes
• 0
1
vote
4
replies
228
views
Tool:
Converting Degree Minutes (DM) to Decimal Degrees (DD) using python script
Minutes
Decimal
offtopic
Degrees
python
updated 23 hours ago by
Ram
43k • written 2 days ago by
kuttibiotech2009
▴ 30
1
vote
2
replies
225
views
Why does assigning genes with biomart give me different values than using a transcripts_to_genes.txt file?
biomart
RNAseq
kallisto
1 day ago by
bioinfo
▴ 150
2
votes
4
replies
224
views
Help with IGV abbreviation
igv
updated 3 days ago by
Ram
43k • written 3 days ago by
GeneC
• 0
3
votes
3
replies
224
views
Getting differential ChIP-seq peaks between conditions after calling consensus peaks
MAnorm
ChIP-seq
consensus
peaks
updated 1 day ago by
ATpoint
82k • written 2 days ago by
Alexandra
• 0
1
vote
1
reply
221
views
Differential Expression with limma: Contrast and Design Matrix, combinatorial approach valid?
limma
updated 2 days ago by
dariober
14k • written 3 days ago by
Holly
▴ 10
0
votes
2
replies
221
views
Inquiry about deseq2 transformation
RNA-seq
deseq2
1 day ago by
Chen
• 0
2
votes
3
replies
218
views
ggrepel function: geom_text_repel()
ggplot2
ggrepel
R
updated 23 hours ago by
Ram
43k • written 1 day ago by
sooni
▴ 20
3
votes
1
reply
216
views
Long reads and fixing of mate-pair issues/marking duplicates with samtools
mate-pair
samtools
alignment
updated 4 days ago by
aw7
▴ 300 • written 8 days ago by
Zeng Hao
▴ 40
1
vote
5
replies
215
views
Circos Plot to Visualize Chromatin Accessibility Changes from ATAC-seq Data
circos
atacseq
updated 2 days ago by
Pierre Lindenbaum
161k • written 2 days ago by
biology_inform
▴ 50
1
vote
2
replies
214
views
Doubt about the process of annotation, detection, identification and classification of transposable elements
annotation
TransposableElements
2 days ago by
MarcosCosta
• 0
2
votes
3
replies
213
views
RSeQC : infer_experiment.py Error: Could not retrieve index file
RSeQC
RNA-Seq
updated 3 days ago by
Ram
43k • written 3 days ago by
Prawesh
• 0
0
votes
3
replies
211
views
Biomart issue, why so few 3'utrs?
utr
biomart
updated 3 days ago by
GenoMax
142k • written 3 days ago by
RNAseqer
▴ 270
0
votes
2
replies
210
views
Output file of samtools flagstat empty
samtools-flagstat
updated 4 days ago by
colindaven
6.4k • written 5 days ago by
ramendra.sarma
• 0
182 results • Page
2 of 4
Recent Votes
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Answer: How to download dbSNP153 vcf files in hg19/GRCH37 version
Produce PCA bi-plot for 1000 Genomes Phase III - Version 2
Produce PCA bi-plot for 1000 Genomes Phase III - Version 2
Answer: TFs and Gene databases
A: Is there a software that can compare phylogenetic trees to each othe (face to fa
Comment: Differentially Expressed Genes between two conditions (scRNA, single GEO dataset
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• 0
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▴ 170
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Recent Replies
Answer: Design for complex RNA-Seq experiment using Deseq2
by
swbarnes2
14k
This is a rather small experiment, I don't know that you can establish a meaningful baseline for each patient with a single pre sample, and…
Comment: GG Sankey plot
by
LauferVA
4.2k
@bk11 i changed this comment to an answer. if you disagree please let me know. thanks for sharing your knowledge. VAL
Comment: ComplexHeatmap - How to change fontsize of rowAnnotation
by
hannes.bongartz
• 0
Thanks for the input. Unfortunately, that didn't help either. I appreciate any other tips. Thank you
Answer: GG Sankey plot
by
bk11
★ 2.5k
You need to change your data table into a 'long' format. You can do something like this- GOs_childs_recursive <- GOs_childs_recursive …
Answer: Trimming tool
by
ntsopoul
▴ 60
I use Trim Galore which automatically recognizes adaptors. https://github.com/FelixKrueger/TrimGalore Here is the script I am using for…
Answer: Trimming tool
by
GenoMax
142k
There are plenty. `bbduk.sh` from BBMap suite (https://jgi.doe.gov/data-and-tools/software-tools/bbtools/bb-tools-user-guide/bbduk-guide/ )…
Comment: ComplexHeatmap - How to change fontsize of rowAnnotation
by
bk11
★ 2.5k
Please check out [in this link.][1] [1]: https://github.com/jokergoo/ComplexHeatmap/issues/349
Comment: Reconstruction of locus, order contigs
by
dthorbur
★ 2.0k
[Here](https://www.biostars.org/p/9544689/) is a forum post that may answer your question.
Comment: zero TPM for many gene including housekeeping when using kallisto
by
ashkan
▴ 160
@atpoint I checked all transcripts of GAPDH. they all have 0 TPM.
Comment: how to treat the replicates while performing WGCNA
by
andres.firrincieli
3.6k
> 1) I do not want to merge the MEs rather I asked about replicates, as > some replicates of same sample are showing different behavior for…
Comment: How to systematically check if a bam file is truncated
by
alanh
▴ 170
For more details about what `samtools quickcheck` actually checks for, add a few `-v`'s to the command line. $ samtools quickcheck -…
Comment: How to handle duplicated genes in TCGA data?
by
Zhenyu Zhang
★ 1.2k
Second what is said above, ensembl_id is the unique identifier. As a good bioinformatics habit, always use unique identifier for your anal…
Comment: ComplexHeatmap - How to change fontsize of rowAnnotation
by
hannes.bongartz
• 0
It doesn't work. The annotation labels are still in a default fontsize. I cant find a way to set the fontsize.
Comment: ComplexHeatmap - How to change fontsize of rowAnnotation
by
Ram
43k
Yes. The solution to most things with ComplexHeatmap is to play around with parameters.
Comment: Reconstruction of locus, order contigs
by
BATMAN
• 0
I do not want to re-annotate the genome. I ask if there is a way to extract the region aligned with the mauve, in .gbk format.
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