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43 results • Page
1 of 1
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Views
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Replies
11
votes
13
replies
6.7k
views
7 follow
log2 fold change in RNA-seq analysis
fold
log2
change
updated 15 hours ago by
May Ling
• 0 • written 2.4 years ago by
wmsalsah
▴ 10
7
votes
13
replies
800
views
7 follow
Forum:
Ideal PC configurations and operating system for bioinformatics laboratory
PC
updated 16 hours ago by
i.sudbery
19k • written 4 weeks ago by
Estevão
▴ 10
0
votes
9
replies
510
views
ComplexHeatmap - How to change fontsize of rowAnnotation
ComplexHeatmap
updated 8 hours ago by
ATpoint
82k • written 6 days ago by
hannes.bongartz
• 0
8
votes
9
replies
3.0k
views
7 follow
Venter Genome Vcf
vcf
updated 5 hours ago by
ericrkofman
▴ 20 • written 10.1 years ago by
win
▴ 980
3
votes
7
replies
3.9k
views
Difference in Bismark output methylation call files and coverage files
RRBS
Bismark
DNA-methylation
updated 15 hours ago by
Papyrus
★ 2.9k • written 3.9 years ago by
linelr
▴ 40
2
votes
6
replies
283
views
How to get the ratio of allele counts from GATK derived VCF file?
Freebayes
GATK
SNP
VCF
23 hours ago by
anikcropscience
▴ 230
1
vote
6
replies
281
views
samtools write-index
samtools
updated 19 hours ago by
aw7
▴ 310 • written 2 days ago by
LucisTheFather
• 0
4
votes
6
replies
319
views
Mouse or Rat Gene Expression Data Similar to GTEx
whole-body
expression
atlas
11 hours ago by
Shicheng Guo
★ 9.4k
1
vote
5
replies
208
views
Error with BiocParallel. No barcodes files found
Barcodes
scRNA-seq
SingleCellExperiment
updated 15 hours ago by
Pierre Lindenbaum
162k • written 19 hours ago by
NTerway
• 0
2
votes
5
replies
231
views
Z score
Z-score
updated 15 hours ago by
Ram
44k • written 22 hours ago by
Akash D
▴ 60
1
vote
4
replies
388
views
some error in building kraken2 database
metagenome
kraken2
updated 18 hours ago by
GenoMax
142k • written 4 weeks ago by
Art1ess
• 0
2
votes
4
replies
1.2k
views
Is it possible to run CibersortX with a very small number of samples?
cibersortx
19 hours ago by
Aspire
▴ 330
0
votes
4
replies
367
views
how to treat the replicates while performing WGCNA
clustering
WGCNA
replicates
9 hours ago by
mavy
▴ 10
5
votes
4
replies
5.1k
views
6 follow
Classic threshold for log2 fold change in RNA-seq experiment
RNA-Seq
updated 21 hours ago by
May Ling
• 0 • written 3.9 years ago by
Mozart
▴ 330
0
votes
3
replies
190
views
What is a good way to do gene differentials in single cell data where one group is small 90 cells and the other group 30,000 cells or 2000 cells.
cell
RNA-Seq
single
updated 8 hours ago by
ATpoint
82k • written 10 hours ago by
shahzaibali
• 0
1
vote
3
replies
217
views
Error when looping over multiple columns in a data frame in R
Statistics
R
updated 9 hours ago by
Jeremy
▴ 910 • written 1 day ago by
Mohamed Samir
▴ 30
7
votes
3
replies
13k
views
Z score in RNAseq
RNA-Seq
Zscore
updated 22 hours ago by
Akash D
▴ 60 • written 6.5 years ago by
rob.costa1234
▴ 310
0
votes
2
replies
612
views
minfi::getQC - is default badsamplecutoff of 10.5 always appropriate? ~half of samples fail by this measure
getqc
ewas
minfi
updated 15 hours ago by
June
• 0 • written 10 months ago by
rkb965
• 0
0
votes
2
replies
150
views
What do the transcript variant # mean in RefSeq?
refseq
updated 11 hours ago by
Ram
44k • written 14 hours ago by
curious
▴ 750
1
vote
2
replies
191
views
Construction of circos plot from WGS data
WGS
Circos
1 hour ago by
Anitha
▴ 10
1
vote
2
replies
133
views
How to interpret this plotMDS of three disease clusters?
microarray
plotMDS
DEGs
updated 18 hours ago by
ATpoint
82k • written 21 hours ago by
egascon
• 0
1
vote
2
replies
138
views
Unexpected read length from NGS
NGS
Illumina
updated 18 hours ago by
ATpoint
82k • written 20 hours ago by
QX
• 0
0
votes
2
replies
154
views
CreateSeuratObject taking very long
seurat
3 hours ago by
eae6d2e7
• 0
0
votes
2
replies
201
views
How do we do quantification using stringtie merge option for all the merged samples generated.
stringtie
quantification
updated 20 hours ago by
i.sudbery
19k • written 2 days ago by
Varsha
• 0
0
votes
1
reply
19
views
Forum:
Does prokka work the same on forward and reverse complementary reads?
Prokka
updated 18 minutes ago by
igmin
• 0 • written 52 minutes ago by
Maksim
• 0
0
votes
1
reply
244
views
GWAS Phenotypes
GWAS
updated 21 hours ago by
韩雨
• 0 • written 5 weeks ago by
solomoncharles77
▴ 90
1
vote
1
reply
105
views
Cellranger-multi : Demultiplexing and Analyzing 5’ Immune Profiling Libraries Pooled with Hashtags
5InmuneProfiling
nf-core
scRNAseq
cellranger-multi
Nextflow
updated 18 hours ago by
GenoMax
142k • written 21 hours ago by
azeu
▴ 10
0
votes
1
reply
123
views
Free AI for R programming
R
updated 15 hours ago by
Ram
44k • written 22 hours ago by
mohamadzare6022
▴ 10
0
votes
1
reply
116
views
Flag multiple filtering steps on VCF files using VEP
VEP
VCF
updated 15 hours ago by
Ram
44k • written 1 day ago by
avelarbio46
▴ 30
0
votes
1
reply
91
views
minfi::getQC - badsamplecutoff 10.5
getqc
minfi
methylation
EPICv2
updated 15 hours ago by
Papyrus
★ 2.9k • written 15 hours ago by
June
• 0
0
votes
1
reply
85
views
BEAST2.5 Chloroplast Divergence Time using Fossil Records
BEAST2.5
Fossil
updated 15 hours ago by
Ram
44k • written 15 hours ago by
a.bibek52
▴ 10
0
votes
1
reply
106
views
Trying to understand warning from MACS2 about too few paired peaks and differing results in DiffBind
MACS2
ATACseq
DiffBind
updated 11 hours ago by
jared.andrews07
★ 17k • written 15 hours ago by
atan
• 0
4
votes
1
reply
123
views
PCA plot no distinct cluster. Only p < 0.05 indicates significant DEGs, while adj p < 0.05 does not.
RNA-seq
padj
p-value
updated 10 hours ago by
swbarnes2
14k • written 15 hours ago by
May Ling
• 0
0
votes
1
reply
76
views
Tools for chromosomal aneuploidy detection
genetics
aneuploidy
NGS
human
updated 1 hour ago by
cmdcolin
★ 3.8k • written 18 hours ago by
adarsh_pp
▴ 40
0
votes
0
replies
49
views
abundance_estimates_to_matrix.pl in Trinity is not generating any matrices / output
trinity
updated 10 hours ago by
GenoMax
142k • written 10 hours ago by
jway
• 0
0
votes
0
replies
93
views
Job:
2 PhD Students in single-cell bioinformatics
NGS
14 hours ago by
mtabaka
• 0
2
votes
0
replies
102
views
Job:
Permanent Position as Researcher/Computational Biologist at the National Genomics Infrastructure (Sweden)
Genomics
Sequencing
Sweden
Stockholm
SciLifeLab
15 hours ago by
Matthias Zepper
4.6k
0
votes
0
replies
37
views
Forum:
Trouble with PLINK's logistic regression analysis and covariatesTrouble with PLINK's logistic regression analysis and covariates
PLINK
GWAS
SNP
5 hours ago by
F110152169
• 0
0
votes
0
replies
36
views
How to calculate nucleotide diversity (mtDNA, PacBio_data), Suggest me some tools
NucleotideDiversity
mtDNA
PacBio
3 hours ago by
hashim.rana11
▴ 20
0
votes
0
replies
69
views
VEP annotation tool: "." isn't numeric in addition (+)
VEP
VCF
updated 15 hours ago by
Ram
44k • written 20 hours ago by
emmanouil.a
▴ 120
0
votes
0
replies
52
views
Assigning Micro-C read pairs to nucleosomes called from MNase-seq data?
Hi-C
MNase-Seq
22 hours ago by
Linus
• 0
0
votes
0
replies
63
views
GWAS or QTL mapping in RIL population
GWAS
RIL
20 hours ago by
韩雨
• 0
0
votes
0
replies
59
views
16S analysis for specie of bacteria identification
Species-identification
16S
Kraken2
updated 9 hours ago by
Ram
44k • written 10 hours ago by
Christopher
▴ 10
43 results • Page
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Cellranger-multi : Demultiplexing and Analyzing 5’ Immune Profiling Libraries Pooled with Hashtags
A: Venter Genome Vcf
Answer: seqtk subseq in.fastq list.txt > out.fastq not extracting full sequence from
Answer: PCA plot no distinct cluster. Only p < 0.05 indicates significant DEGs, while ad
Random Access remote BAM files
Answer: PCA plot no distinct cluster. Only p < 0.05 indicates significant DEGs, while ad
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Answer: Does prokka work the same on forward and reverse complementary reads?
by
igmin
• 0
Prokka works the same on both forward and reverse complementary reads. It doesn't matter if your genetic sequences are in their original fo…
Comment: Construction of circos plot from WGS data
by
Anitha
▴ 10
Thank you for the information. But, I am still struggling like which data to use and how to process the input data to represent to build th…
Comment: Tools for chromosomal aneuploidy detection
by
cmdcolin
★ 3.8k
is there any reason why you do not like tools based on read depths?
Comment: CreateSeuratObject taking very long
by
eae6d2e7
• 0
Thank you for your reply! I followed your advice to convert to sparse matrix first but I got this error Error in Matrix::sparseMatrix(raw_…
Answer: Venter Genome Vcf
by
ericrkofman
▴ 20
I can't find an hg38 version, and the old VCF seems to be of a different header format so I am having difficulty converting it to hg38 from…
Comment: What is a good way to do gene differentials in single cell data where one group
by
ATpoint
82k
Preferred if you have true biological replicates. Can still be combined with my subsetting strategy, like use 100x different cells for the …
Answer: ComplexHeatmap - How to change fontsize of rowAnnotation
by
ATpoint
82k
```r set.seed(1) m = matrix(rnorm(100), nrow = 10) rownames(m) = 1:10 # normal ha = rowAnnotation(foo = anno_mark(at=1:nrow(m), labels=row…
Comment: ComplexHeatmap - How to change fontsize of rowAnnotation
by
Ram
44k
Please do not add answers unless you're actually answering the question. I've moved your post to a comment.
Comment: ComplexHeatmap - How to change fontsize of rowAnnotation
by
hannes.bongartz
• 0
I still cannot find a way. It seems to be an easy to solve issue and I was trying any possible combination with the aforementioned gp=gpar(…
Comment: What is a good way to do gene differentials in single cell data where one group
by
fracarb8
★ 1.6k
I would probably use a pseudobulk approach.
Comment: Error when looping over multiple columns in a data frame in R
by
Jeremy
▴ 910
`cutpoint_results[[col_name]]` adds each new cut point to the list entitled "cutpoint_results", while keeping the original column names fr…
Answer: PCA plot no distinct cluster. Only p < 0.05 indicates significant DEGs, while ad
by
swbarnes2
14k
Never use uncorrected p-values with RNASeq. Your PCA is suggesting that your sample groups are not very different, and the DEG tests con…
Comment: What is a good way to do gene differentials in single cell data where one group
by
ATpoint
82k
I would keep it transparent. Do DE by subsetting large to small group. Do that randomly many times, then either average stats or use some s…
Answer: Trying to understand warning from MACS2 about too few paired peaks and differing
by
jared.andrews07
★ 17k
Without looking at the actual sequence for each genome, my guess is that the Echinobase reference just matches much more closely to the org…
Comment: What do the transcript variant # mean in RefSeq?
by
Ram
44k
> It does not seem to be the longest transcript. Is it the canonical transcript? Longest == canonical. If you're looking for the transcrip…
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