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117,152 results • Page
2 of 2344
Sort: replies
Rank
Views
Votes
Replies
61
votes
51
replies
22k
views
9 follow
Tool:
Efficiently process (view, analize, clip ends, convert, demultiplex, dereplicate) SFF/FastQ files
sff
sequence
fastq
next-gen
updated 11 months ago by
Ram
44k • written 10.1 years ago by
BioApps
▴ 790
94
votes
50
replies
53k
views
16 follow
PCA plot from read count matrix from RNA-Seq
RNA-Seq
pca
updated 2.5 years ago by
BioNovice247
▴ 20 • written 6.6 years ago by
rachel.kubik12
▴ 220
12
votes
50
replies
4.2k
views
How can I find out the Linux version and what is the ftp command to install R and R Studio on Linux server?
R
7.2 years ago by
thomas.f.hahn2
▴ 100
188
votes
50
replies
74k
views
41 follow
How To Remove The Same Sequences In The Fasta Files?
fasta
sequence
duplicates
updated 20 months ago by
Ram
44k • written 13.6 years ago by
Zhangleisdau
▴ 340
22
votes
50
replies
17k
views
Why I can not install anything on R?
R
software error
5.6 years ago by
Za
▴ 140
27
votes
50
replies
18k
views
7 follow
Extract fasta sequences from a large file using a list of names
fasta
updated 15 months ago by
Ram
44k • written 9.1 years ago by
fhsantanna
▴ 620
26
votes
50
replies
14k
views
8 follow
Tool:
Retrieve a subset of FASTA from large Illumina multi-FASTA file
fasta
multi-fasta
illumina
updated 11 months ago by
Ram
44k • written 8.0 years ago by
hcwang
▴ 50
93
votes
50
replies
16k
views
17 follow
Forum:
Fda Sends A Warning Letter To 23Andme - Personal Genomics Service Marketing To Be Discontinued
personal-genomics
23andMe
updated 15 months ago by
Ram
44k • written 10.5 years ago by
Istvan Albert
100k
85
votes
49
replies
10k
views
31 follow
Which Application Is Truly Missing In Bioinformatics?
subjective
general
updated 6 months ago by
Ram
44k • written 14.2 years ago by
Jarretinha
3.4k
262
votes
48
replies
23k
views
37 follow
Forum:
Bioinformatics courses, workshops or training
Workshop
Training
Courses
updated 15 months ago by
Lars
★ 1.0k • written 9.0 years ago by
Deepak Tanwar
★ 4.2k
131
votes
48
replies
16k
views
19 follow
Justifying Learning Linux For Bioinformatics
linux
subjective
updated 12.8 years ago by
Guangchuang Yu
★ 2.6k • written 12.8 years ago by
Eric Normandeau
11k
72
votes
48
replies
18k
views
15 follow
Tutorial:
For Short Reads, Which Aligners Find All Hits Given Certain Edit Distance Threshold?
ngs
alignment
bwa
updated 15 months ago by
Ram
44k • written 11.3 years ago by
lh3
33k
39
votes
48
replies
7.1k
views
CUTADAPT in windows
sequencing
updated 15 months ago by
Ram
44k • written 9.1 years ago by
zizigolu
★ 4.3k
90
votes
48
replies
34k
views
29 follow
Tutorial:
Extract Total Non-Overlapping Exon Length Per Gene With Bioconductor
fpkm
rna-seq
bioconductor
updated 6 months ago by
Ram
44k • written 10.6 years ago by
Irsan
★ 7.8k
72
votes
48
replies
8.6k
views
18 follow
What Kind Of Bioinformatics Tutorials Would You Like To See Online?
education
updated 15 months ago by
Ram
44k • written 13.1 years ago by
User 59
13k
68
votes
48
replies
8.9k
views
16 follow
Forum:
Stranger Things: unexpected limitations of popular tools
samtools
hisat2
updated 12 months ago by
Ram
44k • written 6.9 years ago by
Istvan Albert
100k
18
votes
47
replies
4.8k
views
Fastq Trimmer by pattern
fastq
trimming
reads
terminal
7.3 years ago by
dzisis1986
▴ 70
22
votes
47
replies
5.4k
views
(solved) I couldn't reproduce the problem of max_target_seqs
blast
updated 5.2 years ago by
gb
★ 2.2k • written 5.7 years ago by
fishgolden
▴ 510
23
votes
47
replies
7.2k
views
6 follow
Dbsnp Individual Genotyping Information For Specific Snps
dbsnp
updated 13.2 years ago by
Khader Shameer
18k • written 13.2 years ago by
Andrea_Bio
★ 2.8k
188
votes
47
replies
3.1k
views
16 follow
Job:
Bioinformatics freelancers needed
freelancer
updated 13 months ago by
Ram
44k • written 8.1 years ago by
Bioinfexperts
▴ 190
126
votes
46
replies
51k
views
21 follow
Forum:
Visualization of ChIP-seq data using Heatmaps (Updated: 06/10/16)
visualization
ChIP-Seq
homer
ngsplot
deeptools
updated 13 months ago by
Ram
44k • written 8.2 years ago by
Sinji
★ 3.2k
43
votes
46
replies
4.3k
views
taking sequences of a list of miRNAs
miRNA
mirbase
sequence
gene
updated 7.6 years ago by
Ram
44k • written 7.6 years ago by
zizigolu
★ 4.3k
105
votes
46
replies
13k
views
13 follow
Forum:
Why Does Biostar Cover Questions On Epigenetics, But Not Intelligent Design?
epigenetics
updated 15 months ago by
Ram
44k • written 10.7 years ago by
ugly.betty77
★ 1.1k
37
votes
46
replies
7.1k
views
12 follow
Quick Programming Challenge: How Do I Calculate Reference Coverage From A Table Of Alignment Starts And Ends?
code
alignment
programming
updated 5.7 years ago by
Ram
44k • written 14.1 years ago by
Jeremy Leipzig
22k
176
votes
46
replies
19k
views
26 follow
Forum:
Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
6 months ago by
Istvan Albert
100k
92
votes
46
replies
12k
views
28 follow
Best Language For Introductory Programming Course From Within An Introduction Course On Bioinformatics.
programming
subjective
updated 15 months ago by
Ram
44k • written 12.9 years ago by
Andra Waagmeester
3.2k
85
votes
46
replies
9.4k
views
15 follow
Tool:
ChatGPT optimized for bioinformatics questions
gpt
updated 10 weeks ago by
LauferVA
4.2k • written 11 months ago by
Sasha
▴ 850
237
votes
45
replies
72k
views
34 follow
What Is The Best Pipeline For Human Whole Exome Sequencing?
next-gen
sequencing
illumina
exome
human
updated 12.9 years ago by
User 9126
▴ 50 • written 14.0 years ago by
Biomed
5.0k
185
votes
45
replies
32k
views
27 follow
How To Organize A Pipeline Of Small Scripts Together?
pipeline
general
updated 7 months ago by
Ram
44k • written 14.3 years ago by
Giovanni M Dall'Olio
28k
4
votes
45
replies
7.9k
views
SAM file wrong? help with validatesamfile
EXOME
updated 13 months ago by
Ram
44k • written 7.9 years ago by
cristina_sabiers
▴ 110
22
votes
45
replies
14k
views
9 follow
Tool to separate human and mouse rna seq reads
RNA-Seq
next-gen-sequencing
updated 16 months ago by
Ram
44k • written 9.0 years ago by
Ron
★ 1.2k
108
votes
45
replies
87k
views
30 follow
How To Extract A Sequence From A Big (6Gb) Multifasta File ?
fasta
updated 21 months ago by
aswinssoman
▴ 80 • written 11.8 years ago by
Mchimich
▴ 320
21
votes
44
replies
4.4k
views
How to analyze Illumina RNASeq data
RNA-Seq
sequencing
next-gen
3.8 years ago by
Kumar
▴ 170
46
votes
44
replies
25k
views
9 follow
bash loop for alignment RNA-seq data
RNA-Seq
next-gen
alignment
updated 20 months ago by
Ram
44k • written 10.1 years ago by
Paul
★ 1.5k
122
votes
44
replies
11k
views
24 follow
News:
Help Make Biostar Better! Add Your Feedback, Opinions And Suggestions.
meta
biostars
updated 7 months ago by
Ram
44k • written 11.5 years ago by
Istvan Albert
100k
197
votes
44
replies
72k
views
33 follow
Tutorial:
Analysing Microarray Data In Bioconductor
microarray-analysis
bioconductor
updated 16 months ago by
Ram
44k • written 11.7 years ago by
Obi Griffith
20k
99
votes
44
replies
24k
views
33 follow
Best Free Text Editor For All Popular Languages (R, Python, Perl Etc..)
text
r
perl
python
updated 6.7 years ago by
grant.hovhannisyan
★ 2.6k • written 12.2 years ago by
John
★ 1.5k
155
votes
44
replies
32k
views
29 follow
Which Bioinformatic Friendly Pipeline Building Framework?
scripting
updated 23 months ago by
Ram
44k • written 10.4 years ago by
Carlos Borroto
★ 2.1k
27
votes
44
replies
15k
views
How to remove contamination from the transcriptome assembly
RNA-Seq
contamination
assembly
blast
Hiseq reads
updated 21 months ago by
Ram
44k • written 8.6 years ago by
seta
★ 1.9k
130
votes
44
replies
34k
views
16 follow
Forum:
Trimming adapter sequences - is it necessary?
NGS
adapter
RNA-Seq
updated 14 months ago by
Ram
44k • written 7.7 years ago by
Lars
★ 1.0k
121
votes
44
replies
21k
views
21 follow
What Is Your Favorite Question To Ask When Interviewing Potential Bioinformaticians?
subjective
updated 15 months ago by
Ram
44k • written 13.9 years ago by
Madelaine Gogol
5.3k
90
votes
44
replies
61k
views
28 follow
Tool to generate proportional Venn Diagrams?
visualization
updated 2.1 years ago by
Ram
44k • written 13.1 years ago by
Ryan D
★ 3.4k
43
votes
44
replies
6.3k
views
14 follow
Macbook M1 pro/M2 pro or Dell (with Ubuntu/other linux distribution) for bioinformatics stuff?
metagenomics
amplicon
macbook
Intel
MacOs
updated 7 months ago by
steve
★ 3.5k • written 7 months ago by
l.gallucci
▴ 10
102
votes
44
replies
9.2k
views
22 follow
What Are Numbers Every Bioinformatician Should Know?
knowledge
career
updated 15 months ago by
Ram
44k • written 10.8 years ago by
brentp
24k
185
votes
44
replies
84k
views
30 follow
Drawing Chromosome Ideograms With Data
ideogram
visualization
chromosome
updated 9 weeks ago by
Michael
54k • written 14.2 years ago by
Yuri
★ 1.7k
16
votes
43
replies
6.8k
views
Stuck on calculating principal components
Vegan
R
software error
SNP
updated 6.5 years ago by
pfs
▴ 280 • written 6.5 years ago by
zizigolu
★ 4.3k
31
votes
43
replies
20k
views
11 follow
Volcano plot help code
R
rna-seq
updated 5.9 years ago by
jordi.planells
▴ 480 • written 6.1 years ago by
anasofiamoreira94
▴ 80
66
votes
43
replies
27k
views
23 follow
Ngs - Huge (Fastq) File Parsing - Which Language For Good Efficiency ?
next-gen
sequencing
parsing
fastq
programming
updated 2.3 years ago by
Ram
44k • written 13.4 years ago by
toni
★ 2.2k
59
votes
43
replies
21k
views
18 follow
Tutorial:
Polish PacBio assembly with latest PacBio tools : an affordable solution for everyone
pbalign
pacbio
arrow
smrt-limk
updated 14 months ago by
Ram
44k • written 6.7 years ago by
Rox
★ 1.4k
57
votes
43
replies
16k
views
8 follow
Forum:
Best RNA-Seq aligner: A comparison of mapping tools
NGS
alignment
genome
RNA-Seq
sequence
updated 14 months ago by
Ram
44k • written 5.3 years ago by
David Langenberger
11k
117,152 results • Page
2 of 2344
Recent Votes
A: Most Popular and fancy SNP analysis tools Comparison: Plink, SNPassoc, SNPRelate
Most Popular and fancy SNP analysis tools Comparison: Plink, SNPassoc, SNPRelate
GWAS analysis tool
Answer: Cut&Run and heatmap
Comment: Functional enrichment analysis for unique gene IDs
Answer: How do i calculate the mean of triplicates in a data.frame based on pattern?
How do i calculate the mean of triplicates in a data.frame based on pattern?
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Popular Question
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devenvyas
▴ 740
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schlogl
▴ 160
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22k
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Recent Replies
Comment: txt file to bigwig
by
ATpoint
82k
Please stop asking questions in existing threads. Delete these two comments and open a new question with all the necessary details to under…
Comment: Venter Genome Vcf
by
Jeremy Leipzig
22k
there are some violations in this VCF: - Invalid count number, with fixed count the number should be 1 or higher: key=INFO name=TSA type=St…
Comment: Cut&Run and heatmap
by
sogand
• 0
Hi all, I am new to cut&run or any peak-related analysis and appreciate any guidance on my issue here: I have analyzed peakcalling .txt …
Comment: Functional enrichment analysis for unique gene IDs
by
Pegasus
▴ 100
Since the locus-tags are unclassified, I used the CDS instead of the locus-tags to retrive their ensembl IDs using ensembl bacteria website…
Comment: samtools mpileup error - 1 samples in 1 input files
by
Damla
• 0
Could you find a solution to your problem?
Comment: txt file to bigwig
by
sogand
• 0
Hi all, I am new to these kind of analysis and appreciate any guidance on my issue here: I have analyzed peakcalling .txt files that are r…
Answer: Help me with Heatmap
by
MolGeek
▴ 50
Hello! So, what is the correct design ? You will have to ask yourself, what do you want to compare? WT vs mut? If so it will be strain. 2…
Answer: Help me with simple data for RNA seq
by
swbarnes2
14k
1) you have to say what question you are actually asking. There are few different designs that would address different questions. 2) work…
Comment: identify the coordinate for coding and non_coding region.
by
Ghada
• 0
mmmm I am not sure. This is how I generate my consnsus sequence # Get consensus fastq file samtools mpileup -uf KT992094.1.fasta…
Comment: identify the coordinate for coding and non_coding region.
by
GenoMax
142k
Why do you have those N's at the beginning of the sequence? If the remainder of the sequence matches 100% then the initial N's may be wrong…
Answer: Salmon vs Kallisto vs RSEM
by
Rob
6.6k
It's nice that you've gone ahead and done some analysis of these results and their differences on your data yourself. These more general qu…
Comment: Python script to query GeneCards to get EntrezID, symbol from Ensembl geneID
by
Mike Smith
★ 2.0k
In this location there is an "entrez" file which maps ensembl IDs to Entrez. It's probably as definitive as you're going to find: https://…
Comment: Python script to query GeneCards to get EntrezID, symbol from Ensembl geneID
by
Anya
• 0
Tried it just recently with almost the same results. 456 out of 496 IDs for protein-coding genes returned as "NA" even though I can see NCB…
Answer: Download All The Bacterial Genomes From Ncbi
by
2284046470
• 0
wget https://ftp.ncbi.nlm.nih.gov/genomes/refseq/bacteria/assembly_summary.txt awk -F '\t' '{if($12=="Complete Genome" && $11=…
Answer: How do i calculate the mean of triplicates in a data.frame based on pattern?
by
dariober
14k
I'm a happy user of [data.table](https://rdatatable.gitlab.io/data.table/): ``` library(data.table) dat <- structure(list(sgrna = c("Cont…
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