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116,460 results • Page
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News:
Course - Agent-Based Modelling Using NetLogo - University of Lodz, Poland
Netlogo
IndividualBasedModels
AgentBasedModels
10 minutes ago by
carlopecoraro2
★ 2.5k
2
votes
2
replies
53
views
Assume "yes" as the answer to any prompts with bioconda
docker
container
bioconda
updated 20 minutes ago by
Juke34
8.5k • written 1 hour ago by
njornet
▴ 20
0
votes
0
replies
21
views
10x VDJ (TCR/BCR) BAM to fastq
TCR
VDJ
BCR
10x
1 hour ago by
predeus
★ 1.9k
0
votes
0
replies
16
views
Tutorial:
DNA Methylation: Sequencing Techniques
WGBS
RRBS
58 minutes ago by
Novogene
▴ 410
0
votes
1
reply
315
views
How to add rowLinks, rowTree in SummarizedExperiment manually.
tree
phylogenetic
summarized
microbiome
updated 1 hour ago by
Akshay
• 0 • written 19 months ago by
Muhammad
• 0
0
votes
0
replies
28
views
DEseq2
RNA
DESeq2
seq
2 hours ago by
rrehimi
• 0
0
votes
1
reply
78
views
Korean human genome reference file
GWAS
reference
imputation
updated 2 hours ago by
colindaven
6.3k • written 3 hours ago by
SeoGyun
• 0
1
vote
3
replies
108
views
SLURM submission problem: Indexing the Reference Genome is not done in STAR
align
RNA-Seq
linux
STAR
updated 28 minutes ago by
Dave Carlson
★ 1.7k • written 2 hours ago by
n_navy
• 0
0
votes
2
replies
113
views
RNA seq differential expression analysis
Regarding
seq
data
normalization
RNA
2 hours ago by
rrehimi
• 0
0
votes
5
replies
149
views
How can I retrieve wheat lysine non-acetylated sequences from the UniProt database?
UniProt
updated 53 minutes ago by
me
▴ 750 • written 2 hours ago by
Jacky
• 0
795
votes
167
replies
141k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 12 weeks ago by
Biostar
2.6k • written 7.4 years ago by
Istvan Albert
100k
0
votes
0
replies
41
views
How to compare the quality of assemblies
nextdenovo
assembly
hifiasm
pacbio
updated 2 hours ago by
colindaven
6.3k • written 2 hours ago by
kirillkirilenko
▴ 20
0
votes
0
replies
40
views
How can I obtain the tissue or sample name alongside the Tau score in tspex?
tau
score
tspex
3 hours ago by
bioinfo223
▴ 10
0
votes
0
replies
49
views
How to root an unrooted tree with a known root tree?
phylogenetics
rooting
R
3 hours ago by
P.
• 0
0
votes
0
replies
55
views
Issues with SnpEff Assuming Circular Chromosomes in Eukaryotic Genome Analysis
snpEff
6 hours ago by
ekirsch
• 0
0
votes
0
replies
80
views
Load a full GFF3 into annotation track using arrow (Apollo)
arrow
python-apollo
Apollo
10 hours ago by
renan.igor
• 0
1
vote
1
reply
150
views
Pediococcus acidilactici sequence analysis
BLAST
Gene-Ontology
updated 6 hours ago by
Ram
43k • written 12 hours ago by
anasjamshed
▴ 120
3
votes
1
reply
524
views
What is the normalization status of metabric data?
metabric
normalization
microarray
cbioportal
updated 11 hours ago by
etiennedanis
▴ 20 • written 19 months ago by
DareDevil
★ 4.3k
0
votes
0
replies
74
views
filter the most significant ligand and receptors in netVisual_bubble in cell chat
cellchat
12 hours ago by
synat.keam
▴ 100
0
votes
1
reply
164
views
assembly using CCS, CLR, CCS_CLR sequences together?
assembly
updated 14 hours ago by
GenoMax
141k • written 14 hours ago by
ycts
• 0
1
vote
3
replies
356
views
DEG analysis of RNA-seq data across multiple tissues and two conditions
RNA-seq
EdgeR
DEGs
DESeq2
updated 15 hours ago by
swbarnes2
14k • written 17 hours ago by
BioinfGuru
★ 1.7k
1
vote
1
reply
193
views
Inconsistency in SNP detection pipelines for multi-sample analysis
vcf
bam
bcf
snps
updated 16 hours ago by
LChart
3.9k • written 18 hours ago by
George
▴ 10
0
votes
0
replies
94
views
Problem with Mirdeep2 and Randfold output
mirdeep2
Randfold
updated 16 hours ago by
Ram
43k • written 16 hours ago by
otieno43
▴ 20
0
votes
2
replies
215
views
Per base sequence content failed miserably
fastqc
sequence
trimmomatic
NGS
assembly
2 hours ago by
Kai Xin
• 0
1
vote
1
reply
157
views
Retrieve a % coverage for each transcript
RNA-seq
updated 18 hours ago by
Ram
43k • written 18 hours ago by
jammydodger123456
▴ 40
0
votes
1
reply
276
views
Pruning Phylogenetic Trees and Bootstrap Values
phylogenetics
bootstrap
updated 18 hours ago by
Klaus S
▴ 150 • written 6 days ago by
Zeng Hao
▴ 40
0
votes
2
replies
246
views
How can I solve this error?
metal
8 hours ago by
22211020193
• 0
0
votes
0
replies
94
views
Struggling to lift genome builds
PLINK
liftover
GWAS
updated 18 hours ago by
GenoMax
141k • written 19 hours ago by
srimmer
• 0
1
vote
2
replies
188
views
Can I readmap short reads to rDNA references?
rDNA
assembly
updated 18 hours ago by
Ram
43k • written 19 hours ago by
aniigodwinn
• 0
0
votes
2
replies
197
views
Sheep reference genome remapping coordinates
reference
remap
sheep
coordinates
genome
updated 19 hours ago by
colindaven
6.3k • written 1 day ago by
valentinatsar
• 0
1
vote
3
replies
201
views
Editing Human Reference Genome by adding a CDS
gff
agat
transcriptome
updated 19 hours ago by
Michael
54k • written 20 hours ago by
LDT
▴ 330
0
votes
8
replies
374
views
Questions about a bug when transferring cram file to bam file
sequence
samtools
bcftools
updated 18 hours ago by
Pierre Lindenbaum
161k • written 21 hours ago by
me
• 0
0
votes
1
reply
210
views
differential gene expression analysis when not all samples have an untreated counterpart
differential-expression
edgeR
updated 22 hours ago by
Gordon Smyth
★ 7.0k • written 8 days ago by
nhaus
▴ 300
0
votes
0
replies
161
views
Looking for an explanation as to what the Peak Score is from HOMER, not finding any answers on Biostars yet
Homer
ATAC-seq
22 hours ago by
Ronin
• 0
4
votes
2
replies
1.7k
views
How to convert SAM/BAM file to GTF/GFF file?
GTF
SAM
BAM
GFF
minimap2
updated 23 hours ago by
Dr.Animo
▴ 130 • written 11 months ago by
BioinfoBee
• 0
2
votes
6
replies
501
views
Removing duplicates
duplicates
ONT
minimap2
3 hours ago by
quentinperriere
• 0
0
votes
1
reply
140
views
Annotating a dataset with gnomAD v4 using Hail
Python
Sequencing
Hail
Exome
Annotation
updated 23 hours ago by
DKA
▴ 40 • written 1 day ago by
james.melhorn
• 0
3
votes
4
replies
209
views
biomaRt error for different R version
biomaRt
R
4 minutes ago by
manaswwm
▴ 490
0
votes
1
reply
129
views
Freyja plot error
Freyja
updated 20 hours ago by
Ram
43k • written 1 day ago by
Adyasha
• 0
1
vote
2
replies
145
views
Interpretation of combined P values - RNA microarray meta analysis
metaanalysis
R
microarray
DExMA
combinedP
1 day ago by
hagl
▴ 10
0
votes
0
replies
93
views
BIOM format can contain tabular data ?
BIOM
phyloseq
biomformat
R
1 day ago by
chemokine-1
▴ 10
0
votes
4
replies
351
views
How to reduce the variation of CIBERSORTx results?
CIBERSORTx
deconcolution
4 hours ago by
feather-W
• 0
0
votes
1
reply
155
views
Job:
Consultant to update a Snakemake workflow with the new Kubernetes executor plugin for Snakemake 8
Kubernetes
DevOps
executor
AWS
Snakemake
updated 1 day ago by
Michael
54k • written 1 day ago by
jonrkarr
▴ 100
0
votes
2
replies
162
views
Bedmethyl file format
bedmethyl
methylation
updated 23 hours ago by
GenoMax
141k • written 1 day ago by
njornet
▴ 20
0
votes
0
replies
116
views
sequence table for DADA2 chimera filtering
OTU
sequencetable
DADA2
1 day ago by
Ali
• 0
2
votes
4
replies
249
views
SnpEff annotates coding duplication as intronic?
snpeff
1 day ago by
kirill.zaslavsky
• 0
0
votes
2
replies
188
views
Calculating percentage of cells proliferating in single cell rna seq data
statistics
scRNA-seq
proliferation
1 day ago by
mropri
▴ 150
0
votes
1
reply
212
views
How to get the reference panel for UKBB
UKBB
GWAS
clump
updated 18 hours ago by
bk11
★ 2.3k • written 1 day ago by
航太郎
• 0
5
votes
4
replies
2.8k
views
genefu for PAM50 prediction
RNA-Seq
breast cancer
subtyping
updated 1 day ago by
hamarillo
▴ 70 • written 5.6 years ago by
silviajserrano
▴ 50
3
votes
10
replies
559
views
Piping samtools to R
R
NGS
bash
samtools
1 day ago by
joe
▴ 500
116,460 results • Page
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Recent Votes
Answer: What is the normalization status of metabric data?
Answer: Assume "yes" as the answer to any prompts with bioconda
Answer: Assume "yes" as the answer to any prompts with bioconda
Answer: biomaRt error for different R version
Answer: biomaRt error for different R version
Answer: Pediococcus acidilactici sequence analysis
C: How to convert 10X single nuclear RNA Seq bam file to fastq?
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Recent Replies
Comment: biomaRt error for different R version
by
manaswwm
▴ 490
Wonderful, and thanks for tagging Mike! For those who come back for this problem, the following seemed to work for me: Downgrading `dbply…
Answer: Assume "yes" as the answer to any prompts with bioconda
by
Juke34
8.5k
From my experience using `biocontainers` base container no need to set `yes` installation is done without any command prompt, otherwise as …
Comment: SLURM submission problem: Indexing the Reference Genome is not done in STAR
by
Dave Carlson
★ 1.7k
You're allocating 4 compute nodes (`#SBATCH -N 4`), but STAR can't actually make use of more than 1 compute node. You can increase the num…
Answer: Assume "yes" as the answer to any prompts with bioconda
by
Dave Carlson
★ 1.7k
```conda install -y bioconda::packageX conda-forge::packageY``` should do what you're looking for.
Comment: Supergnova stuck in tutorial
by
Murat
• 0
Hi GenoMax, Thank you for your advice. I sent an email to them and have already opened an issue on Github. Let see if there is any other so…
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
by
me
▴ 750
You can't. The data does not exist. UniProt only annotates where a lysine is know to be acetylated. There is no way that UniProt can know w…
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
by
Jacky
• 0
Yes, that's what I meant.
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
by
Jacky
• 0
I'm sorry, I didn't explain it clearly. Below are my operational steps: I enter 'Triticum aestivum' in the search box and perform a search.…
Comment: Indexing the Reference Genome is not done in STAR
by
n_navy
• 0
I add these parameters; #SBATCH -N 4 #SBATCH --tasks=1 #SBATCH -c 4 #SBATCH --time=24:00:00 But it keeps giving error
Comment: RNA seq differential expression analysis
by
rrehimi
• 0
Thanks! Could you please explain the DEseq2 method using Galaxy step by step? Input files?
Answer: Korean reference file
by
colindaven
6.3k
Is is this one - KOREF ? There might be multiple versions. https://www.ncbi.nlm.nih.gov/bioproject/PRJNA294231/ If it is, then take that…
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
by
me
▴ 750
The question is not quite clear? are you asking for wheat proteins in UniProtKB which contain a lysine amino-acid, where those lysine are *…
Comment: RNA seq differential expression analysis
by
seta
★ 1.9k
You should provide row count (without normalization) for doing DEG analysis by relevant tools such as, edgeR or DEseq.
Comment: GSEA analysis in R
by
Sudip
• 0
Thank you. I have another question. Is there a way to get the gene list under "setSize" from the output that you gave as an example.
Comment: Per base sequence content failed miserably
by
Kai Xin
• 0
Hi, i have added the fastqc plots (untrimmed and trimmed). The sample was from anaerobes enriched from wastewater treatment plant, and this…
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