Entering edit mode
4.9 years ago
sarfrajiiet
•
0
i have rna paired fasta file I trimmed it using trim galore and then mapped it with hg38 bowtie2 index now i have sam file is it important to sort it for htseq count i have to ask that the process that i follow till now is it correct differential expression and second that what next to do to get differential expression i m new please help me
Please add some punctuation and write in separate sentences. This is barely readable.
bowtie2 does not due spliced alignment, but you did not tell us which organism you are working on. For eukaryotic organisms, STAR or HISAT2 would be a better choice.
Yes. A better tool would be featureCounts.