Transform BED file to SAF for featureCounts

Question

[Chironex]

hello! I have this bed file:

    KQ415659.1      0       34      Simple_repeat_1     .       +
    KQ415659.1      35      64      Simple_repeat_2     .       +
    KQ415659.1      1759    1822    SINE/tRNA-Deu_1     .       +
    KQ415659.1      1901    2059    DNA/hAT-Charlie_1   .       -
    KQ415659.1      2112    2258    DNA/hAT-Charlie_2   .       +
    KQ415659.1      2890    2964    Simple_repeat_3     .       +
    KQ415659.1      4085    4130    Low_complexity_1    .       +
    KQ415659.1      5587    5619    Simple_repeat_4     .       +
    KQ415659.1      5931    5995    SINE/tRNA-Deu_2     .       -
    KQ415659.1      6448    6475    Simple_repeat_5     .       +
    KQ415659.1      6556    6588    Simple_repeat_6     .       +
    KQ415659.1      6588    6622    Simple_repeat_7     .       +
    KQ415659.1      6622    6633    Simple_repeat_8     .       +
    KQ415659.1      6665    6787    SINE/MIR_1          .       +

and I would to transform it into a SAF file because featurecounts doesn't recognize it as a right format. What are the steps to transform it? thank you

Answer 1

[ATpoint]

Please use the search function: Converting from BED to SAF/GFF