Finding a biomarker from DE genes

Question

[Rituriya]

Hi All,

I have around say 150 differentially expressed (DE) genes between 2 groups (control and treatment of a disease) by RNA-seq. My goal is to really shorten this list and find that few number of genes, which are of utmost significance which acts as a marker for this disease.

Now my problem is that there are so many ways out there to achieve this (read quite a lot of papers) and no one states it clearly. Can anyone suggest the best way to shortlist really significant (biologically and statistically) gene/genes between these groups?

Thank you, Rituriya.

Answer 1

[Buffo]

There are many ways to determine if a set of genes are functionally related, and/or altered as group in response to a "disease", you can start with webgestalt or string. These tools may help you to understand/stablish networks between DE genes.