Methods to determine if a nanopore 1-D read is from the forward or reverse strand without a reference?

Question

[BioinformaticsLad]

I am guessing the answer is no, but would it be theoretically possible to do so?

Answer 1

[WouterDeCoster]

No, that's not possible. There is no biological or biochemical difference those strands, it's just an arbitrary convention based on the reference genome.