Markduplicates did not find output file with glob pattern
1
0
Entering edit mode
5.1 years ago
yifan wang ▴ 20 
[workflow ] starting step markDuplicates
[step markDuplicates] start
[job markDuplicates] /tmp/28h_j0bq$ docker \
    run \
    -i \
    --volume=/tmp/28h_j0bq:/pXbDAg:rw \
    --volume=/tmp/q2gfy6t7:/tmp:rw \
    --volume=/tmp/zzuqc4mx/samtools-sort-2019-03-21.bam:/var/lib/cwl/stg871a2789-0abb-4d3c-8244-3d5f0c32d923/samtools-sort-2019-03-21.bam:ro \
    --workdir=/pXbDAg \
    --read-only=true \
    --user=1000:1000 \
    --rm \
    --env=TMPDIR=/tmp \
    --env=HOME=/pXbDAg \
    --cidfile=/tmp/asp7gqb5/20190328124012-712750.cid \
    kfdrc/picard \
    java \
    -Xmx4g \
    -jar \
    /picard.jar \
    MarkDuplicates \
    INPUT=/var/lib/cwl/stg871a2789-0abb-4d3c-8244-3d5f0c32d923/samtools-sort-2019-03-21.bam \
    OUTPUT=markDuplicates-2019-03-21.bam \
    METRICS_FILE=metricsFile-markDuplicate2019-03-21.metrics \
    REMOVE_DUPLICATES=true \
    CREATE_INDEX=true \
    TMP_DIR= \
    /home/bpvast/01.test/CWL/tmp \
    'ASSUME_SORTED= TRUE'
04:40:16.316 INFO  NativeLibraryLoader - Loading libgkl_compression.so from jar:file:/picard.jar!/com/intel/gkl/native/libgkl_compression.so
[Thu Mar 28 04:40:16 GMT 2019] MarkDuplicates INPUT=[/var/lib/cwl/stg871a2789-0abb-4d3c-8244-3d5f0c32d923/samtools-sort-2019-03-21.bam] OUTPUT=markDuplicates-2019-03-21.bam METRICS_FILE=metricsFile-markDuplicate2019-03-21.metrics REMOVE_DUPLICATES=true ASSUME_SORTED=false TMP_DIR=[/home/bpvast/01.test/CWL/tmp] CREATE_INDEX=true    MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true ADD_PG_TAG_TO_READS=true DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX=<optimized capture="" of="" last="" three="" ':'="" separated="" fields="" as="" numeric="" values=""> OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
[Thu Mar 28 04:40:16 GMT 2019] Executing as ?@83bc93520372 on Linux 4.4.0-116-generic amd64; OpenJDK 64-Bit Server VM 1.8.0_191-b12; Deflater: Intel; Inflater: Intel; Picard version: 2.17.4-SNAPSHOT
INFO    2019-03-28 04:40:16 MarkDuplicates  Start of doWork freeMemory: 996964168; totalMemory: 1011351552; maxMemory: 3817865216
INFO    2019-03-28 04:40:16 MarkDuplicates  Reading input file and constructing read end information.
INFO    2019-03-28 04:40:16 MarkDuplicates  Will retain up to 13832844 data points before spilling to disk.
INFO    2019-03-28 04:40:22 MarkDuplicates  Read     1,000,000 records.  Elapsed time: 00:00:05s.  Time for last 1,000,000:    5s.  Last read position: chr1:100,133,238
INFO    2019-03-28 04:40:22 MarkDuplicates  Tracking 3065 as yet unmatched pairs. 164 records in RAM.
INFO    2019-03-28 04:40:30 MarkDuplicates  Read     2,000,000 records.  Elapsed time: 00:00:13s.  Time for last 1,000,000:    8s.  Last read position: chr1:217,630,900
INFO    2019-03-28 04:40:30 MarkDuplicates  Tracking 5944 as yet unmatched pairs. 91 records in RAM.
INFO    2019-03-28 04:40:35 MarkDuplicates  Read     3,000,000 records.  Elapsed time: 00:00:18s.  Time for last 1,000,000:    4s.  Last read position: chr2:112,389,864
INFO    2019-03-28 04:40:35 MarkDuplicates  Tracking 14358 as yet unmatched pairs. 688 records in RAM.
INFO    2019-03-28 04:40:39 MarkDuplicates  Read     4,000,000 records.  Elapsed time: 00:00:22s.  Time for last 1,000,000:    4s.  Last read position: chr3:28,324,280
INFO    2019-03-28 04:40:39 MarkDuplicates  Tracking 16546 as yet unmatched pairs. 937 records in RAM.
INFO    2019-03-28 04:40:45 MarkDuplicates  Read     5,000,000 records.  Elapsed time: 00:00:28s.  Time for last 1,000,000:    5s.  Last read position: chr3:187,253,301
INFO    2019-03-28 04:40:45 MarkDuplicates  Tracking 18539 as yet unmatched pairs. 80 records in RAM.
INFO    2019-03-28 04:40:50 MarkDuplicates  Read     6,000,000 records.  Elapsed time: 00:00:32s.  Time for last 1,000,000:    4s.  Last read position: chr5:10,652,890
INFO    2019-03-28 04:40:50 MarkDuplicates  Tracking 20629 as yet unmatched pairs. 1234 records in RAM.
INFO    2019-03-28 04:40:54 MarkDuplicates  Read     7,000,000 records.  Elapsed time: 00:00:37s.  Time for last 1,000,000:    4s.  Last read position: chr6:19,838,477
INFO    2019-03-28 04:40:54 MarkDuplicates  Tracking 22013 as yet unmatched pairs. 1269 records in RAM.
INFO    2019-03-28 04:40:58 MarkDuplicates  Read     8,000,000 records.  Elapsed time: 00:00:41s.  Time for last 1,000,000:    4s.  Last read position: chr7:21,880,732
INFO    2019-03-28 04:40:58 MarkDuplicates  Tracking 22902 as yet unmatched pairs. 1567 records in RAM.
INFO    2019-03-28 04:41:05 MarkDuplicates  Read     9,000,000 records.  Elapsed time: 00:00:47s.  Time for last 1,000,000:    6s.  Last read position: chr8:474,764
INFO    2019-03-28 04:41:05 MarkDuplicates  Tracking 23426 as yet unmatched pairs. 1479 records in RAM.
INFO    2019-03-28 04:41:09 MarkDuplicates  Read    10,000,000 records.  Elapsed time: 00:00:52s.  Time for last 1,000,000:    4s.  Last read position: chr9:40,265,240
INFO    2019-03-28 04:41:09 MarkDuplicates  Tracking 23622 as yet unmatched pairs. 1320 records in RAM.
INFO    2019-03-28 04:41:13 MarkDuplicates  Read    11,000,000 records.  Elapsed time: 00:00:56s.  Time for last 1,000,000:    4s.  Last read position: chr10:50,125,119
INFO    2019-03-28 04:41:13 MarkDuplicates  Tracking 23447 as yet unmatched pairs. 1237 records in RAM.
INFO    2019-03-28 04:41:18 MarkDuplicates  Read    12,000,000 records.  Elapsed time: 00:01:00s.  Time for last 1,000,000:    4s.  Last read position: chr11:47,733,976
INFO    2019-03-28 04:41:18 MarkDuplicates  Tracking 22944 as yet unmatched pairs. 1636 records in RAM.
INFO    2019-03-28 04:41:22 MarkDuplicates  Read    13,000,000 records.  Elapsed time: 00:01:05s.  Time for last 1,000,000:    4s.  Last read position: chr12:10,801,951
INFO    2019-03-28 04:41:22 MarkDuplicates  Tracking 22136 as yet unmatched pairs. 2172 records in RAM.
[Thu Mar 28 04:41:30 GMT 2019] picard.sam.markduplicates.MarkDuplicates done. Elapsed time: 1.23 minutes.
Runtime.totalMemory()=3666345984
To get help, see http://broadinstitute.github.io/picard/index.html#GettingHelp
Exception in thread "main" htsjdk.samtools.util.RuntimeIOException: java.nio.file.NoSuchFileException: /home/bpvast/01.test/CWL/tmp/sortingcollection.3061849251471313486.tmp
    at htsjdk.samtools.util.SortingCollection.spillToDisk(SortingCollection.java:247)
    at htsjdk.samtools.util.SortingCollection.add(SortingCollection.java:167)
    at picard.sam.markduplicates.MarkDuplicates.buildSortedReadEndLists(MarkDuplicates.java:524)
    at picard.sam.markduplicates.MarkDuplicates.doWork(MarkDuplicates.java:232)
    at picard.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:269)
    at picard.cmdline.PicardCommandLine.instanceMain(PicardCommandLine.java:98)
    at picard.cmdline.PicardCommandLine.main(PicardCommandLine.java:108)
Caused by: java.nio.file.NoSuchFileException: /home/bpvast/01.test/CWL/tmp/sortingcollection.3061849251471313486.tmp
    at sun.nio.fs.UnixException.translateToIOException(UnixException.java:86)
    at sun.nio.fs.UnixException.rethrowAsIOException(UnixException.java:102)
    at sun.nio.fs.UnixException.rethrowAsIOException(UnixException.java:107)
    at sun.nio.fs.UnixFileSystemProvider.newByteChannel(UnixFileSystemProvider.java:214)
    at java.nio.file.Files.newByteChannel(Files.java:361)
    at java.nio.file.Files.createFile(Files.java:632)
    at java.nio.file.TempFileHelper.create(TempFileHelper.java:138)
    at java.nio.file.TempFileHelper.createTempFile(TempFileHelper.java:161)
    at java.nio.file.Files.createTempFile(Files.java:852)
    at htsjdk.samtools.util.IOUtil.newTempPath(IOUtil.java:317)
    at htsjdk.samtools.util.SortingCollection.newTempFile(SortingCollection.java:256)
    at htsjdk.samtools.util.SortingCollection.spillToDisk(SortingCollection.java:221)
    ... 6 more
[job markDuplicates] Max memory used: 303416MiB
[job markDuplicates] Job error:
Error collecting output for parameter 'mark-Dups_output':
03.PICARD-Markdup.cwl:128:7: Did not find output file with glob pattern: '['markDuplicates-2019-03-21.bam']'
[job markDuplicates] completed permanentFail
[step markDuplicates] Output is missing expected field file:///home/bpvast/01.test/CWL/GATK-CWL-WorkFlow-201903/GATK-v1.0-Workflow.cwl#markDuplicates/mark-Dups_output
[step markDuplicates] completed permanentFail
cwl • 2.6k views
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3
Entering edit mode

The TMP_DIR is set wrong /home/bpvast/01.test/CWL/tmp is a path outside the container. Yifan, how is the parameter set? Using $(runtime.tmpdir)?

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0
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i think you are right, i used to set tmp as ./tmp run by individual step. i will have a try using $(runtime.tmpdir) and ./tmp

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1
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Hello Yifan Wang, can you fix the formatting? There should be a 101010 like button that will help.

What cwltool version are you using? Can you share a link or the contents of the CWL file itself?

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0
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  1. home/bpvast/anaconda3/bin/cwltool 1.0.20190228200117

  2. https://github.com/Johnnywang92/GATK-CWL-WorkFlow-201903/blob/master/GATK-v1.0-Workflow.cwl

  3. yesterday i did as your suggestion, the markDuplicates-2019-03-21.bam did not show in mycache

and i upload the error message at https://github.com/Johnnywang92/GATK-CWL-WorkFlow-201903/blob/master/Error

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1
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Caused by: java.nio.file.NoSuchFileException: /home/bpvast/01.test/CWL/tmp/sortingcollection.3061849251471313486.tmp

looks like a IO error

do you have enough space in your tmp directory /home/bpvast/01.test/CWL/tmp ?

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1
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My understanding is that cwltool stages all the files necessary for workflow execution in a temporary directory, then erases said directory and only keeps the output. So the tool is not supposed to do any IO outside of said directory during runtime, right? Seeing the java code would be very helpful.

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3
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The Java code is doing nothing wrong, check the cmdline

java \
    -Xmx4g \
    -jar \
    /picard.jar \
    MarkDuplicates \
    INPUT=/var/lib/cwl/stg871a2789-0abb-4d3c-8244-3d5f0c32d923/samtools-sort-2019-03-21.bam \
    OUTPUT=markDuplicates-2019-03-21.bam \
    METRICS_FILE=metricsFile-markDuplicate2019-03-21.metrics \
    REMOVE_DUPLICATES=true \
    CREATE_INDEX=true \
    TMP_DIR= \
    /home/bpvast/01.test/CWL/tmp \
    'ASSUME_SORTED= TRUE'

It was told to use the wrong TMP_DIR

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0
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yes, it is. I have enough space in the tmp directory

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4
Entering edit mode
5.1 years ago

In CWL we never pass a filesystem paths, we only used File and Directory types for that, never a string.

In your particular case you are setting the temporary directory for your tools: https://github.com/Johnnywang92/GATK-CWL-WorkFlow-201903/blob/650ac1743f1a6e259b3e0f9b19baed65916b6432/GATK-v1.0-Workflow.cwl#L21

This is not wrong! But please use the CWL method for this.

1) remove your input named tmp as linked to above.

2) For each step that references this tmp parameter, remove it and upgrade the CommandLineTool it calls.

For example, https://github.com/Johnnywang92/GATK-CWL-WorkFlow-201903/blob/650ac1743f1a6e259b3e0f9b19baed65916b6432/03.PICARD-Markdup.cwl#L65

Remove the tmpdir input parameter and add a new section to arguments:

arguments:
- valueFrom: /picard.jar
  position: 2
  prefix: -jar
- valueFrom: MarkDuplicates
  position: 3
- TMP_DIR=$(runtime.tmpdir)

3) Now that all the tools are upgraded, configure the temporary path of your workflow runner (if needed). For cwltool check it's --help output.
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