basic experimental methods book for bioinformaticians

Question

[Pappu]

I am wondering if the is any basic experimental methods book or review for bioinformaticians so that they can understand the presentations given by experimentalists. Such book should include what kind of experimental methods can be used to validate what kind of in-silico analysis results for example differentially expressed genes, SNPs, pathway enrichment, biomarker targets etc.

[h.mon]

sentimentalists

What are sentimentalists?

[JC]

A simple book could be hard to create, each methodology is based on a lot of biological knowledge. Even biologists don't understand the methods they do, many experiments are based on "faith". Coming from a wet lab I know many people cannot explain the methods, like "don't do a PCR on a moon night because it will not work" or "sing a lullaby to the plant embryos to have a better transformation rate".

[Joe]

I don’t think there is likely to be a satisfactory answer to this question, but the Gospel of molecular biology which you’ll find all 3 volumes of in any self-respecting mol bio lab is Sambrook et al: https://hum-molgen.org/literature/12-2000/000001.html

If there’s a molecular technique (qPCR, cloning, protein work etc.) its in that book (or I should say books).