Entering edit mode
12.5 years ago
Genotepes
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950
Hi,
I know this is not the best place to ask this but I have spotted some population geneticists that may help when I went trought the questions.
I have three occurences of the same mutation, each lying on a haplotypes of micro-satellites. I am asked to say whether there is evidence for recurrent mutations - 2 individuals whare alleles and one is shares no common haplotype.
I tried astimating age of mutation and hoped to provs some incompatibility with allele frequency but because I am in a situation where information is very low (3 microsat t=which arereally "close" genetically), I find it hard to give a strong support for any of the alternatives.
Would you have a method (and even software) to propose ?
Christian
Some more detail might be useful. Are these de novo events? Are the individuals related? How "close" are the microsatellites? Any other data for other individuals?
Hi,
three individuals are "unrelated" - as far as we know (one is from different country). Each one has relatives (nuclear family) where affected share the haplotype - this is how I was able to reconstruct the haplotype.
Two microsatellites are very close < 0.5 cM on the "left" and 0.1 cM on the right. The other span on 10cM, which is a bit large for this kind of study.
MS1-MS2-MS3 -MS4 - MS5-mutation-MS6 - MS7-MS8-MS9 0 0.1 0.15 2.6 6 6.6 6.8 7.3 8.9 cM
This map is not ideal, at least on the right side but I had to make do with it.
Christian