How to fix column order with pheatmap?

Question

[Alicia]

Dear all, I want to generate a pheatmap with rlog values from DEseq, and I want to put the samples with higher expression value to the right side, and the samples with lower expression values to the left side. Therefore I can compare the results from different experiments. Do you have any suggestions how to make that happen?

[Arup Ghosh]

Sort the columns based on expression and use cluster_cols=FALSE.

[Alicia]

Hi, arup, thanks a lot.

Answer 1

[cpad0112]

as mentioned in this post, How to regroup columns in R using heatmap.2 or pheatmap ?, reorder the matrix as per your requirement and then as arup said below, do not cluster the cols. @ Alicia

[Alicia]

Thank you very much. I missed the post you mentioned when I searched it. The method is quite strightforward.

[cpad0112]

np. upvote the original post if it is helpful. Alicia