Canary output subsequent analysis
0
0
Entering edit mode
5.8 years ago
Myosotis1979 ▴ 30

Dear all I have generated a gvar file using Canary algorithm to call copy number polymorphisms. Then, I generated a summary file of it using PLINK. Here a subset of the file:

        NAME        FIELD        VALUE
        CNP3          CHR            1
        CNP3           BP       777800
        CNP3          CNV           no
        CNP3      ALLELIC           no
        CNP3       GCOUNT           42
        CNP3      INTEGER            Y
        CNP3            A            1
        CNP3          [2]            1
        CNP3          0/0         59:0
        CNP3      AA/null         42:0
        CNP4          CHR            1
        CNP4           BP      1640120
        CNP4          CNV          yes
        CNP4      ALLELIC           no
        CNP4       GCOUNT           43
        CNP4      INTEGER            Y
        CNP4            A            1
        CNP4          [1]      0.02326
        CNP4          [2]       0.9767
        CNP4          0/0         58:0
        CNP4       A/null          1:0
        CNP4      AA/null         42:0
        CNP6          CHR            1
        CNP6           BP      8108521
        CNP6          CNV           no
        CNP6      ALLELIC           no
        CNP6       GCOUNT           41
        CNP6      INTEGER            Y
        CNP6            A            1
        CNP6          [2]            1
        CNP6          0/0         60:0
        CNP6      AA/null         41:0

I have many questions: - How to distinguish which are polymorphic CP ? - How to convert this file into an input file to STRUCTURE (population stratification software) as well as for the PCA analysis? - Should I use all these CNP for linkage desequilibrium analysis or should I filter to those which have "yes" value in this file ? Your help is valuable as I spent a lot of time searching for a tutorial in vain. Many thanks

canary plink Copy number polymorphism • 1.3k views
ADD COMMENT
0
Entering edit mode

Hello, in which format was your original ata? - CEL files? Which platfom? - Affymetrix SNP 6.0?

ADD REPLY
0
Entering edit mode

Hello, CEL files for Affymetrix 6.0 plateforme

ADD REPLY
0
Entering edit mode

okay, and how did you perform Canary? - using Affymetrix Genotyping Console?

ADD REPLY
0
Entering edit mode

I performed Canary according to the BirdSuite pipeline

ADD REPLY
0
Entering edit mode

...and what is the input format required for STRUCTURE? You know that you can do PCA and MDS in PLINK, too?

ADD REPLY
0
Entering edit mode

You already have your data in PLINK PED or BED format, right?

ADD REPLY

Login before adding your answer.

Traffic: 2701 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6