Hi again!
I have another problem with my data. FastQC returns red flag in per tile sequence quality. I see that this problem is often associated with reverse read of sequencing (in few lines). That data are representing WGS.
In other files I see horizontal red line through one tile.
What can I do with it? Should I remove bad tile or what? Thank you for your advices. I attached printscreen from my fastqc.
Ok. Thank you so much! So program for alignment can handle with it?
I thought that I need to do TILE FILTERING using FilterByTile.sh software. So that step is unnecessary? I can normally do alignment with this tiles problem?
I have presented graph after trimming (using trimmomatic). Does it means, that aligner (such as BWA) can handle with this tiles problem?