options for RNA polymerase II-chipseq data using macs2 callpeak
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8.5 years ago
chenzizhen01 ▴ 20

I'm dealing with RNApolyII chipseq data using MACS2.

RNApolyII chipseq data contains two kinds of peaks:narrow peaks at TSS region and broad peaks across genebody.

So, how to set options of MACS2?

DO I have to run MACS2 twice? First, run macs2 callpeak without --broad in order to find narrow peaks at TSS. Then run macs2 with --broad to find broad peaks?

If I do that, should I run MACS2 bdgdiff separately if I want to find difference between two different samples?

ChIP-Seq Assembly • 3.3k views
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