I am trying to analyze some data with the w4Cseq pipeline (https://github.com/WGLab/w4CSeq).
For only some of my sequences I got this error message: ERROR: Requested column 1, but database file all_reads.bed only has fields 1 - 0.
Basically the pipeline was not able to produce the .bed from my reads
I am using:
hg19 as reference
CATG as cutter and the cutter is in my primer.
Do you have any idea what can be wrong?