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Question: 4C-seq analysis using w4Cseq ERROR: Requested column 1, but database file all_reads.bed only has fields 1 - 0.
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Dear,

I am trying to analyze some data with the w4Cseq pipeline (https://github.com/WGLab/w4CSeq).

For only some of my sequences I got this error message: ERROR: Requested column 1, but database file all_reads.bed only has fields 1 - 0.

Basically the pipeline was not able to produce the .bed from my reads

I am using: bwa 0.7.9 samtools 1.6 bedtools 2.26.0 hg19 as reference CATG as cutter and the cutter is in my primer.

Do you have any idea what can be wrong?

Thanks

ADD COMMENTlink 23 months ago ste.lu • 40

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