Off topic:how to add inline barcode to raw DNA fragment without PCR

Question

[liaoyunshi]

Dear all,

I want to use inline barcodes (barcode adjacent to the DNA fragment, included as the starting of the sequencing reads) for my illumina sequencing. Then I found most of current posts used this technique for short amplicon sequencing (e.g. 16S sequencing), in which the inline barcodes can be included during the PCR amplification. However, in my situation, I can only add the inline barcode after PCR and fragmentation, so I wonder if there is any kit or suggestion to add the inline barcode to the end-prep DNA fragment (I think shoule be done by ligation), or where I can design and synthesize the inline barcodes (essentially, short double strand DNA)?

Thanks.