FastQC after trimming adapters
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6.5 years ago
amy16 ▴ 40

I find a big variation in per tile sequence quality before and after trimming of the adapters for PE sequencing. Is this observed usually?
RNA-Seq • 1.6k views
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Please share the screen shots

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Variation for better or worse?

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The Per tile sequence quality has become worse.

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The per tile sequence quality of R2 strand-unpaired has become worse.

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