Question

Automatic functional annotation of protein sequences

0

Entering edit mode

6.7 years ago

Cacau ▴ 520

Is there any tool to automatically annotate eukaryotic genomes? I have protein sequences available already.

blast genome gene protein annotation • 1.6k views

ADD COMMENT • link updated 6.7 years ago by lakhujanivijay 5.8k • written 6.7 years ago by Cacau ▴ 520

1

Entering edit mode

There is software called blast2go which performs blast and then subsequent annotation for given set of sequences.

ADD REPLY • link 6.7 years ago by Chirag Parsania ★ 2.0k

score 2 · Answer 1 · 2017-08-16

Blast2GO is a good and popular option as suggested by Chirag. It's a paid commercial software. An open source alternative would be to perform standalone blastp -> get the description. Use the hit accession to derive the GO ontologies using UniprotKb by retrieving the UniprotKB ids from GI identifiers. You can get a lot of information including the GO annotations and the EC numbers (Enzyme Codes). Additionally, for pathway analysis you can use the KAAS server.