microRNA high-throughout sequencing analysis

Question

[Bei.Liu]

recently I am analyzing the mciroRNA sequencing data, but the problem is that my sequencing data don't have repeat, which means every sample that I sent to company just has been sequenced once. so I was wondering is there any appropriate and better method to analyze the data. thank you

[Devon Ryan]

Technical replicates are largely useless. The question is whether you have biological replicates...

[WouterDeCoster]

You may want to specify what the aim is of your analysis, and how many samples you do have.

Answer 1

[Czh3]

Deseq and edgeR both can handle this.